Yes, I do agree, that is why I call it a tool for people to use. I think that
it is a stereotype to think that histologists are not experienced or
knowledgable about computers. There are some histologists who have had a fairly
good introduction to computer systems, how computers work, what
One of the departments in the University runs a class each year where they
have the student's process, section and stain a batch of tissues. The
microtome's they use have come from Noah's histology lab on the Ark. The
mechanical workshop tries to keep them functional but they are really beyond
Yea, I probably didn't communicate quite clearly enough, but I didn't want
to elaborate too much as I suspect some people may be getting tired of
hearing me talk.
.but yes, invariably in every lab I run into, I find at least one person in
each area who is more knowledgeable about computers
I do agree that computers are tools that are indeed an asset to anatomic
path laboratories. Michael I applaud you for your efforts in getting
the staff and engaging them. This is the basis of my entire theory, in
order to create efficiencies within histology that there are 3 distinct
area of the
I use Mayer's Hematoxlyin for 30-45 secs. Wash for 2 minutes, blue in Ammonia
Water, wash for 5 minutes, dehydrate clear and mount if it is DAB. If it is
AEC I do the same except I rinse in distilled water before mounting with
aqueous mount.
Frances L. Swain HT(ASCP) A. A. S.
Special
Hey everybody!
Today I tried to do a fluorescent staining on cryosectionned tissue
embedded in agar.
I didn't see a problem in the presence of the agar untill I analyzed
the slides under the microscope... The agar had absorbed my
fluorescent dy!!
Does anyone know who to remove the agar
OUCH
JTT
- Original Message -
From: fro...@bitstream.net
To: Jennifer Anderson jander...@halozyme.com
Cc: Histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, March 26, 2009 9:50 PM
Subject: Re: [Histonet] Please help! In dire need of user manuals
Dear Jennifer and any other
The response below is completely and utterly disrespectful and downright rude
Steven Mello,HT(ASCP)
Anatomical Pathology Manager
From: jnoc...@satx.rr.com
To: fro...@bitstream.net; jander...@halozyme.com
Date: Fri, 27 Mar 2009 10:52:53 -0500
Subject: Re: [Histonet] Please help! In dire
Double ouch! Somebody has their panties in a bunch today!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Friday, March 27, 2009 11:53 AM
To: fro...@bitstream.net; Jennifer Anderson
Cc:
has anyone had any trouble when embedding little specimens they float to the
sides of the molds like they are charged. it happens sometimes with our little
core bxs. thanks, everyone have a good weekend!
anita dudley
providence hosp
mobile alabama
The secret of life is honesty and fair dealing. If you can fake that,
you've got it made.
- Groucho Marx,
Double-dog ouch.
Nuff said, I'm backing out now,
William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847
-Original
For those who prepare specimens for ground histology we have plastic slides
available. Since I am closing my lab we will no longer be selling custom
plastic slides. We are closing our stock at half price.
2 x 3 x .030 clear800 slides
2 x 3 x .060 clear64 slides
2 x 3 x .060
I would suggest you send your recycled xylene for testing; the manufacturer
of your recycler should be able to recommend several. I suspect water in the
xylene (I have seen it cause this issue on the processor) or inadequate
drying of the slides before deparaffinization. Any water droplets left on
Flaming? This is total burn out! Very inappropriate.
Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
Histology Day Supervisor
183 E. 8th Ave.
Chico, CA 95926
530-891-6244
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Does anyone out there know of a reference that states or discusses tissues
that a covered with a large amount of excess blood have problems with fixation.
I have looked in the reference materials I have here in the lab, however I
cannot find anything to support my complaints to a client that
Wow. Sorry to say it but this whole manual comeback thing is pretty crazy. Talk
about panties in a bunch. How about switching to decaf! Histonet is a great
resorce for individuals to come for help and/or discussions for ceratin things
including answers, comments and well... I would think
TGIF!
I have an opportunity for a full time/permanent
histotechnologist/histotechnician in *Central Wisconsin.* Please forward
this to anyone you know who may seem fit for this position, and if we place
on of your referrals in a position you will earn a $1000 referral bonus. If
you are interested
found some more plastic slides
2 x 4 x 060 clear164 slides
These we used to true the head on the Exakt grinder.
Cathy Mayton
Wasatch Histo Consultants, Inc.
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Does anyone out there remember who had the nifty sectioning tape for TMA's?
Or does anyone use it? The vendor was at the NSH meeting last fall.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Hi Dears,
It is Friday, but not a weekend yet:-):-(
I just got a request from my PI to figure out:
1. How often now day's people use Trypsin (EDTA, Proteinase K or E)
as an Antigen Retrieval for FFPE.
2. Why or is the Citrate Buffer pH6 more usable???
3. Is Trypsin very
I use Trypsin only as a last resort. I use Pepsin because it is more gentle
(does not chew up the sections). I do not use Proteinase K unless I absolutely
have to and for just a few minutes as it will eat the sections off of the
slides. If I use Citrate Buffer I heat it up in the microwave
The tape is made by instrumedics and the system is the cryojane tape transfer
system.
Christie
From: b-freder...@northwestern.edu
To: histonet@lists.utsouthwestern.edu
Date: Fri, 27 Mar 2009 13:39:06 -0500
Subject: [Histonet] TMA tape sectioning system
Does anyone out there
I, for one, love how he begins all politely with hopefully not too
offensive to those that may be sensitive to such replies before getting
straight to Are you crazy? not even three paragraphs later. This is
classic trolling at its finest... congratulations Ford! I'll be sure to
keep your rant
There is also a paraffin transfer system now it is all from Leica. You can use
the paraffin system with the slides for frozen sections and it works well if
you don't have the money for the whole Cryo Jane system. They purchased the
company that bought Instrumedics last year. It was under
So, there are tons of hematoxylin recipes out there, and many, I find, are
very similar in their staining properties. Yes, Richard Allen manufactures
a modification of Harris' Hematoxylin, which is essentially Harris' recipe
without the mercuric oxide. I don't know how long Richard Allen's
I was wondering how many Histo labs in Australia are working on a 24 hour
system. What have they seen as the advantages but more importantly the
disadvantages with such a system.
P
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I thought the rant was pretty funny.
Just repeat to yourself it's an email list, I should really just relax.
Emily
--
prometheus, thief of light, giver of light, bound by the gods, must have
been a book.
-mark danielewski, house of leaves
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