[Histonet] G 20 MEETING NEWS

2009-04-01 Thread Edwards, R.E.
Apparently Obama and Brown, with the reluctant compliance of Germany and China are aiming to rationalise the pay and conditions of laboratory workers worldwide, and hopefully if they succeed the histotechs in the U.S.A. will one be the major beneficiaries!, so fingers xxxed

RE: [Histonet] Leica Bond Max - any opinions?

2009-04-01 Thread Blazek, Linda
I would really suggest you look closely at the Intellipath from BioCare. They are a truly open system. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com

RE: [Histonet] Leica Bond Max - any opinions?

2009-04-01 Thread Jackie M O'Connor
I agree with Linda. I researched 5 different automated IHC stainers to be used for toxicology research applications, and found the intelliPATH to be the best solution for our needs. Biocare, as well, has a vast array of anti rat and mouse primary antibodies and detection systems.

[Histonet] (no subject)

2009-04-01 Thread Witt, Dan
We currently have a full time job opening at our lab. We are hospital based and are located 15 miles east of St. Louis, mo in in Belleville, IL. If interested contact Dan Witt at dw...@sebh.org. ___ Histonet mailing list

Re: [Histonet] Online SOP's

2009-04-01 Thread Rene J Buesa
Good question, to which you have to add: how ALL the personnel supposed to use the SOP sign-off? It does not take so much space after all, so keep your SOP hard copy and make it sign by all. René J. --- On Tue, 3/31/09, Bauer, Karen L. bauer.ka...@mayo.edu wrote: From: Bauer, Karen L.

Re: [Histonet] Histology Training

2009-04-01 Thread Rene J Buesa
And what kind of a training are you going to receive in 1-2 weeks? Very incomplete I suppose! René J. --- On Tue, 3/31/09, Poole, Kimberly kimberly.po...@drdc-rddc.gc.ca wrote: From: Poole, Kimberly kimberly.po...@drdc-rddc.gc.ca Subject: [Histonet] Histology Training To:

RE: [Histonet] Effects of Cytocool on IHC

2009-04-01 Thread Rene J Buesa
That is another urban legend, spray if you need, no effect on IHC (or anything else) whatsoever. René J. --- On Tue, 3/31/09, Sebree Linda A lseb...@uwhealth.org wrote: From: Sebree Linda A lseb...@uwhealth.org Subject: RE: [Histonet] Effects of Cytocool on IHC To: Leslie McCormack

Re: [Histonet] Histology Training

2009-04-01 Thread Larry Woody
Is that an April Fools joke?   Larry A. Woody Seattle, Wa. From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; Poole, Kimberly kimberly.po...@drdc-rddc.gc.ca Sent: Wednesday, April 1, 2009 5:43:04 AM Subject: Re: [Histonet]

RE: [Histonet] Calcium salts

2009-04-01 Thread Bernice Frederick
Sodium thiosulfate removes excess silver and has been used in the Von Kossa technique for years as well as most,if not all silver stains we do. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611

Re: [Histonet] Leica Bond Max - any opinions?

2009-04-01 Thread Dolores_Fischer
Michelle, We also only work with animal tissue. Have you considered researching Biocare's Intellipath immunostainer? We also love their products and this is an open system. I am not the one who made the purchase decision or used both of the stainers, but, although the Leica immunostainer was

Re: [Histonet] onalighternote

2009-04-01 Thread Bernie Taupin
I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. From: Va Paula Sicurello vapat...@yahoo.com To: Jeanine (CDC/CCID/NCZVED)Bartlett

Re: [Histonet] onalighternote

2009-04-01 Thread Pat Flannery
Then maybe we're really making fun of the other Bernie; we just didn't know it. :-) I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about.

Re: [Histonet] AO Blade holder

2009-04-01 Thread Bernie Taupin
Am I getting multiple mails or is it simply that David Waugh doesn't know how to use a listserv? From: David Waugh dwa...@kent.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 3:08:08 PM Subject: [Histonet] AO Blade holder I have a

Re: [Histonet] onalighternote

2009-04-01 Thread Bernie Taupin
Then maybe we're really making fun of the other Bernie; we just didn't know it. :-) Perhaps you should get back to your histology and just plain stop making fun of anyone, for any reason. From: Pat Flannery pjfne...@duke.edu To:

Re: [Histonet] onalighternote

2009-04-01 Thread Bernie Taupin
I have just had s from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what s are. From: Edwards, R.E. r...@leicester.ac.uk To: histonet@lists.utsouthwestern.edu

RE: [Histonet] onalighternote

2009-04-01 Thread Robyn Vazquez
Please stop replying and use your own advice. Histology talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 6:55 AM To: Edwards, R.E.;

Re: [Histonet] G 20 MEETING NEWS

2009-04-01 Thread Bernie Taupin
Please refrain from discussing politics on this list. Edwards, R.E. wrote: Apparently Obama and Brown, with the reluctant compliance of Germany and China are aiming to rationalise the pay and conditions of laboratory workers worldwide, and hopefully if they succeed the

Re: [Histonet] onalighternote

2009-04-01 Thread Bernie Taupin
Please stop replying and use your own advice. Histology talk anyone? I'm curious- Why didn't you seem to feel this way about the first 3-5 replies that were sent out making fun of my name, completely devoid of histology talk? From: Robyn Vazquez

RE: [Histonet] onalighternote

2009-04-01 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
I enjoy the lighter side of things in my crazy day.and the subject line warns us ahead of time -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01,

RE: [Histonet] onalighternote

2009-04-01 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
Hey Bern, I'm with you! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 10:03 AM To: Robyn Vazquez; Edwards, R.E.; histonet@lists.utsouthwestern.edu

Re: [Histonet] G 20 MEETING NEWS

2009-04-01 Thread Angela Bitting
Yes please. There are plenty of blogs out there for this kind of thing. Bernie Taupin bernietau...@ymail.com 4/1/2009 9:53 AM Please refrain from discussing politics on this list. Edwards, R.E. wrote: Apparently Obama and Brown, with the reluctant compliance of Germany and China are

[Histonet] PBS poll

2009-04-01 Thread anh2006
For those of you doing immunos, do you use PBS with or without Calcium and Magnesium and why did you make that choice? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Region IX Education Day Toronto June 2009

2009-04-01 Thread Mark Elliott
Hi everyone. I forgot to mention in my earlier post about our upcoming Education Day that all the prices quoted are in Canadian dollars, so right now you get a great deal as the Canadian dollar has dropped in value against the US dollar. Mark Elliott Region IX Education Committee Chair

RE: [Histonet] G 20 MEETING NEWS

2009-04-01 Thread Beckham, Sharon
I disagree. This is something I didn't know about and evidently it will effect our profession. No one enjoys getting political more than I do, but I don't consider this as being political talk. I do consider it being newsworthy and something I want to become informed about. Thanks for the

RE: [Histonet] G 20 MEETING NEWS

2009-04-01 Thread Bernie Taupin
Sharon, You might like to know, then, that John McCain thinks histology matters http://lungcancer.einnews.com/article.php?nid=91 Beckham, Sharon wrote: I disagree. This is something I didn't know about and evidently it will effect our profession. No one enjoys getting political more than

[Histonet] FREEZY spray

2009-04-01 Thread Senn, Amy R
I was taught that freeze spray shouldn't be used - ever - on any tissue at all. We were told that it damages the tissue. Am I wrong?? We don't even use it when doing frozens - we have a different system now that extracts all the warmth and freezes the tissue completely so that we don't need

Re: [Histonet] FREEZY spray

2009-04-01 Thread Jennifer MacDonald
Federal registry 1910.1030 “All procedures involving blood or other potential infectious materials shall be performed in such a manner as to minimize splashing, spraying, spattering, or generation of droplets of these substances.” NCCLS Document M29-T (now CLSI) “Frozen sections done on unfixed

[Histonet] IHC, decalcification preferences

2009-04-01 Thread Nicole Collette
Hello, all, I am going to be doing some IHC on adult mouse bone, and since I'm new to all this antigen preservation stuff, was wondering the best way to decalcify? Normally, I use high percentage EDTA (17%) and determine end point by weight loss/weight gain. (up until now have been using

Re: [Histonet] IHC, decalcification preferences

2009-04-01 Thread Amy Porter
All the mouse femurs and tibias done in our laboratory are decaled in 14% EDTA and we have a high success rate with our immunohistochemistry and Tunel staining. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133

[Histonet] Let's call a truce

2009-04-01 Thread Va Paula Sicurello
Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) Paula

Re: [Histonet] AO Blade holder

2009-04-01 Thread David Waugh
Again I apologize for what was an honest mistake. I tried looking at the list server home and could not find information regarding the time it takes a post to appear and rules regarding text formats etc. The page I found had only information on how to subscribe or unsubscribe. If there is

[Histonet] deplasticizing Spurr slides

2009-04-01 Thread Emily Lockman
I still need help with deplasticizing our Spurr slides. Any advice would be helpful. Also, when cutting Spurr sections, what type of slides (charged, coated, ect.) are people using? Thanks, Emily Emily M. Lockman, HT (ASCP) Histotechnologist II, Pathology Services American

RE: [Histonet] Off topic posts

2009-04-01 Thread Jennifer Anderson
Oh my. If I had known my request for manuals would have sparked such diatribe, I would have withheld. Please stop at once - you're unnecessarily filling my Inbox. This is preposterous. The conversation should be over. I thought this list was monitored??? Jennifer Anderson The information

[Histonet] many thank yous for all the excellent advice!

2009-04-01 Thread Andrea Grantham
A few weeks ago I asked on Histonet for suggestions on processing and sectioning insects - cockroaches and mosquito GI tracts. After many emails and quite a few phone calls and some further research I was able to get very excellent sections of both and impress the crap out of the

Re: [Histonet] Off topic posts

2009-04-01 Thread Bernie Taupin
People get a life enough already Yes, thanks for your valuable, helpful insight, Florence. From: Florence Leomiti bat...@ohsu.edu To: Jennifer Anderson jander...@halozyme.com; Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009

[Histonet] looking for a MAC1 antibody (aka CD11b/CD18, or Integrin alpha-M beta-2)

2009-04-01 Thread Merced Leiker
I am looking for a good antibody for MAC-1 (CD11b/CD18, Integrin alpha-M beta-2) that is targeted to rodent tissue. Any ideas where I can find one? Thanks! Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of

[Histonet] Off topic...lets call a truce

2009-04-01 Thread Amy Johnson
Histonetters, I have been watching and reading these posts all day and I find it all very hilarious. What I have read has not been offensive or rude by any means towards any one individual yet there is one out there who thinks he is being attacked and therefore he thinks he needs to retaliate

RE: [Histonet] Off topic...lets call a truce

2009-04-01 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
It's not just you -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 7:09 PM To: Amy Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Off

Re: [Histonet] Off topic posts

2009-04-01 Thread Joe Nocito
yeah, what she said - Original Message - From: Bernice Frederick b-freder...@northwestern.edu To: 'Bernie Taupin' bernietau...@ymail.com; 'Bryan Llewellyn' llewl...@shaw.ca; 'Histonet' histonet@lists.utsouthwestern.edu Sent: Wednesday, April 01, 2009 3:10 PM Subject: RE: [Histonet]

Re: [Histonet] Off topic posts

2009-04-01 Thread Bernie Taupin
What were we talking about again? From: Joe Nocito jnoc...@satx.rr.com To: Bernice Frederick b-freder...@northwestern.edu; Bernie Taupin bernietau...@ymail.com; Bryan Llewellyn llewl...@shaw.ca; Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday,

Re: [Histonet] Off topic posts

2009-04-01 Thread Bryan Llewellyn
My name is Bryan (rhymes with (Ian) not Bruce. I was just making a general comment. Bryan - Original Message - From: Bernie Taupin To: Bryan Llewellyn ; Histonet Sent: Wednesday, April 01, 2009 11:45 AM Subject: Re: [Histonet] Off topic posts Who are you calling rude,

Re: [Histonet] Off topic posts

2009-04-01 Thread Bernie Taupin
You should consider going by Bruce. It suits you better, anyway, as far as I can tell.The last person I knew named Llewellyn was... that guy from The People's Court. Remember that show? http://en.wikipedia.org/wiki/The_People%27s_Court From: Bryan Llewellyn

Re: [Histonet] Let's call a truce

2009-04-01 Thread Bernie Taupin
At this point, I think about 20 or more different people have been involved in this thread! Would anyone else like to chime in with their two-cents about being on-topic or offer their inane peanut-gallery observations and silly tit-for-tat bickering like Paula here?