RE: [Histonet] FREEZY spray

2009-04-08 Thread Tony Henwood
Hey, Would you believe that putting the word Taupin into my outlook rules actually works. Now every obnoxious post from Taupin actually disappears into my Junk folder and I do not have to read the drivel. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager

RE: [Histonet] FREEZY spray

2009-04-08 Thread Kemlo Rogerson
Say thank you Uncle Kemlo!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If

Re: [Histonet] Staying...

2009-04-08 Thread njoydobro
is your name really Bernie Taupin or are? you just a?fan of his? Gene -Original Message- From: Bernie Taupin bernietau...@ymail.com To: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov; Marla Thomas mtho...@littonlab.com; histonet@lists.utsouthwestern.edu Sent: Tue, 7 Apr 2009 11:40

RE: [Histonet] Controls needed!

2009-04-08 Thread Hofecker, Jennifer L
Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William

RE: [Histonet] Controls needed!

2009-04-08 Thread Kemlo Rogerson
It's just a thought, can't you get the control from your Microbiology Lab? You just need to make a cell block out of the bacteria that they've grown; I'm not aware that Hpylori has to go through a human system before you can demonstrate it. Kemlo Rogerson e-mail kemloroger...@nhs.net if not

[Histonet] Benchmark XT

2009-04-08 Thread Phyllis Thaxton
Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with  needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6  hours fixation, 3 hour biopsy processing run on VIP

Re: [Histonet] histonet off-topic comments

2009-04-08 Thread Andrea Grantham
Linda, This really is too much - now I have this thing on my computer maliciously put there by aa aa that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but

[Histonet] UNSUBSCRIBE

2009-04-08 Thread Andrea Grantham
UNSUBSCRIBE Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains

RE: [Histonet] histonet off-topic comments

2009-04-08 Thread Pamela Marcum
AMEN!! Andi - after a while you get tired of first reviewing every message for the good ones and then deleting everything else as fast as you can without opening them. This is too valuable a service to Histology to lose over a few malicious individuals who obviously have no interest in the field

[Histonet] Re: BEWARE aa aa staying with a link

2009-04-08 Thread Akemi Allison-Tacha
Hi,Late last night I saw the posting from  aa aa regarding staying, and there was a link, which I stupidly linked on to.  What a BIG MISTAKE!  It is a musical video.  I started listening to it and it was cute, then I tried to close it. It stayed on, and on, and on!  I have a Mac Book Pro

RE: [Histonet] Re: BEWARE aa aa staying with a link

2009-04-08 Thread Martin, Gary
If you have clicked on the aa aa attachment and can't get out of the loop ... use the ALT control delete buttons pushed simultaneously and you will get to the task manager ... highlight the attachment and click the button that says end task now that should get you out of the loop. Gary

[Histonet] Take care openning ANY attachments or links

2009-04-08 Thread Lester Raff MD
Just a general word of warning. Anytime you are opening email from unknown mailers (including people on listserves) be careful about opening links and attachments. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel

RE: [Histonet] histonet off-topic comments

2009-04-08 Thread Pamela Marcum
Unfortunately some of us don't use our work e-mail address due to rules or convenience. Pam Marcum -Original Message- From: Kevin Gillinder [mailto:k.r.gillin...@newcastle.ac.uk] Sent: Wednesday, April 08, 2009 12:03 PM To: Pamela Marcum; Andrea Grantham; LINDA MARGRAF Cc:

Re: [Histonet] Benchmark XT

2009-04-08 Thread Dana Spears
I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick.

AW: [Histonet] Benchmark XT

2009-04-08 Thread Gudrun Lang
Phyllis, we mainly don't have this problem and have been using the ultraview for at least one year. Do you airdry the slides horizontally? We only have the problem of loosing tissue, when the water under the sections was not moved away well enough. And we often start the run directly after

[Histonet] anti-parvalbumin antibody protocol

2009-04-08 Thread Barrezueta, Nestor
I am looking for a protocol to detect parvalbumin in frozen sections of human brain. Antibody suggestion is also needed. Thanks, Nestor Nestor X. Barrezueta Bristol-Myers Squibb Company Applied Genomics 5 Research Parkway Wallingford, CT 06492-7660 203.677.6353 fax 203.677.3990

[Histonet] Formula 83 users?

2009-04-08 Thread Jacqueline Farnsworth
HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in

FW: [Histonet] Benchmark XT

2009-04-08 Thread Esparza, Sandra
Sandra Sandra Esparza HT(ASCP)QIHC Dell Children's Hospital of Central Texas 4900 Mueller Boulevard Austin, Texas 78723 512-324- x87061 sespa...@seton.org -Original Message- From: Esparza, Sandra Sent: Wednesday, April 08, 2009 12:23 PM To: 'gu.l...@gmx.at' Subject: RE:

RE: [Histonet] MAMMOGLOBIN

2009-04-08 Thread Jodie Robertson
I use the Ultra View Detection kit on the Ventana Benchmark XT with the following protocol: Deparaffinization CC1 Mild Mammaglobin - 32 min. Ultra Wash Hematoxylin - 4 min. Bluing - 4 min. We have very good results. Mammaglobin isn't expressed in every breast case either, so the control could

[Histonet] Michael LaFriniere

2009-04-08 Thread Phyllis Thaxton
Please give me a call stranger...205-759-7487 Phyllis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

AW: [Histonet] Colloidal Iron control

2009-04-08 Thread Gudrun Lang
You can use tissue, that is also alcianblue-positiv. Like skin, aorta, cardilage, umbilical cord.. Renal clearcellcarcinoma (I don't know the exact english term). Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Michael LaFriniere

2009-04-08 Thread Phyllis Thaxton
please call me at work 205-759-7487 Phyllis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: RE: [Histonet] Re: BEWARE aa aa staying with a link

2009-04-08 Thread Jane M Flinn
I had to turn off my PC. Luckily that killed it. jane Life is short - make haste to be kind Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 - Original Message - From:

[Histonet] Negative IHC controls

2009-04-08 Thread Sheila Haas
I have a question concerning ANP.22570 on the CAP checklist. Could you all tell me how you are handling negative controls for IHC staining? The question actually states (and I've confirmed with CAP) that we should be running two types of negative controls. One for reagents and one for each

RE: [Histonet] Benchmark XT

2009-04-08 Thread Kari Bradshaw
We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it

[Histonet] Re: Recycled Formalin

2009-04-08 Thread Miyamoto, Garret T Mr CIV USA USAMEDCOM
- Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009 7:15 pm Subject: Histonet Digest, Vol 65, Issue 17 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe

[Histonet] Formula 83 users?

2009-04-08 Thread Peter Baldwin
I can't speak to its functionality, but per its MSDS it is extremely flammable (FP= 45F) and would, thus be considered a hazardous waste by the EPA.EPA's regulations for all solid hazardous waste (which includes liquids) produced by healthcare facilities and others require that they be

Re: RE: [Histonet] Virus planted ? BEWARE aa aa staying with a link

2009-04-08 Thread Akemi Allison-Tacha
Hi All, I guess I was stupid to link on this file.  I just hope aa aa didn't do this as an act of malicious behavior, and now we have viruses in our computers!  I usually delete any unknown sender, and fortunately my MAC filters out most spams.  Now I will have to do a diagnostic check.  I

[Histonet] hand held pH meter for sale

2009-04-08 Thread Cathy Mayton
I have a Fisher Scientific Accumet hand held pH meter for sale. Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Part-Time Histologist position for private lab in Phoenix

2009-04-08 Thread Jill Cox
Hi Netters, We have a part-time histologist position for a wonderful Dermatology lab here in Phoenix AZ. Hours are flexible, wonderful work environment, great Doctors, please call Jill at 602-481-1424 if interested or would like more info. Jill Cox HT (ASCP)        

Re: [Histonet] histonet off-topic comments

2009-04-08 Thread Sate Hamza
Yes. I use Gmail, because my hospital gives only a mediocre 10 MBs of inbox space for the hospital account. Many others on the list use Yahoo or Gmail, or other provider email accounts. I use this Gmail account to subscribe to 5 or 6 mailing lists relevant to my practice. I have been a member of

Re: [Histonet] Colloidal Iron control

2009-04-08 Thread Sate Hamza
A skin biopsy from a discoid lupus erythematosus (DLE) case. Sate On 4/8/09, Kathleen Boozer booze...@ah.org wrote: What are you using? -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada ___ Histonet mailing list

RE: [Histonet] Colloidal Iron control

2009-04-08 Thread Tony Henwood
Kathleen, Controls: Mucin Control (Appendix/umbilical cord). Also include kidney and skin. A second slide should be left in water until step 6 (to detect endogenous iron) Alcian Blue (pH 2.5) can be run in parallel. Tickoo et al (1998 Am J Surg Pathol 22(4): 419-424) recommend that when

[Histonet] wood slide boxes for sale

2009-04-08 Thread Cathy Mayton
I have 5 nice wood slide boxes that hold 100 slides for sale Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] UNSUBSCRIBE

2009-04-08 Thread Bernie Taupin
I've heard a few other people mention it, but you know that replying UNSUBSCRIBE to the entire list doesn't do anything but send an email containing only the word UNSUBSCRIBE out to all the list-members, yeah? From: Andrea Grantham algra...@email.arizona.edu

Re: [Histonet] FREEZY spray

2009-04-08 Thread Bernie Taupin
I see that Kemlo is an HistoNet charlatan like the rest of us. He's the first complain about off-topic notes to the list, then goes on to send several of his own. Thank you Uncle Kemlo! :-P Way to keep this ball rolling! From: Kemlo Rogerson

[Histonet] unsubscribe

2009-04-08 Thread Christine Jacobs
Please cancel my subscription to histonet CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If

[Histonet] Re: burned tissue

2009-04-08 Thread Shelly Coker
Ann,   We had this problem at one time and found that the tissue was being left out to dry during grossing, and the result was that the sections were actually cutting thicker due to difficulty sectioning.  Is this a possibility for you?  The drying out could also happen at the physicians office

Re: [Histonet] unsubscribe

2009-04-08 Thread Bernie Taupin
You'd think that managing one's subscription to a mailing-list was rocket-science or something! From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov To: Christine Jacobs jac...@mmc.org; histonet@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009

[Histonet] Spyware from an Attachment:

2009-04-08 Thread u know
Dear Listers, If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 that is a virus-proof link, by the way.

[Histonet] TMA for cell pellets?

2009-04-08 Thread Thom Jensen
Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: www.arraymold.com www.beecherinstruments.com Two of the best TMA products on the market. cheers, Date: Thu, 2 Apr 2009 12:05:39 -0400 From: louise_hart...@urmc.rochester.edu To:

Re: [Histonet] TMA for cell pellets?

2009-04-08 Thread Bernie Taupin
Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! From: Thom Jensen tissuear...@hotmail.com To: