[Histonet] slide scanner

Hi, I am looking to buy a slide scanner for highthrouput project. Could you give me your opinion if you are using one of the next 3: Mirax Scan Zeiss, Nanozoomer Hamamatsu and XT Aperio? Best regards Jeanne Jeanne Estabel, PhD MGP Histology Operations Manager Wellcome Trust/Sanger

[Histonet] HRP-labeled primary antibodies

Hi All, Has anyone had experience doing IHC on FFPE tissues with HRP-labeled primary antibodies?  I was wondering what the best way to detect them would be.  I assume that going strait to DAB would not work, since no amplification is there.  I was thinking of using a biotinyl tyramide step to

RE: [Histonet] Gross photography/macrophotography

I have been very happy with the Olympus C-7000. It will focus at 3 1/4 inches (8 cm) in its macro mode. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Wednesday, May 20, 2009 4:53 PM

[Histonet] Bone!

Friends, Can someone recommend to me how to store bones (juvenile rat femur) that I have fixed and decalcified, but am not yet ready to cut (they're for vibratome, so not embedded). Thanks! Redward. ___ Histonet mailing list

[Histonet] bone fixation and calcification

Dear Histonetters, I am new in fixation and processing bones. I need your full protocol with details how to fix and process mice bone to visualize the tumor metastases? Thanks in advance, Naira ___ Histonet mailing list

Re: [Histonet] HRP-labeled primary antibodies

If there is enough of the antigen in the tissue or sample you can detect without amplification. HRP/DAB is an enzymatic reaction so it is also is amplification. I would try it the straight up way before diving into more complex protocols. Alternatively another option would be that you could

Re: [Histonet] HRP-labeled primary antibodies

I got some great ideas.  Thanks everyone! Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: anh2...@med.cornell.edu anh2...@med.cornell.edu To: Kim Merriam kmerriam2...@yahoo.com; Histonet histonet@lists.utsouthwestern.edu; ih...@googlegroups.com Sent:

[Histonet] bone tissue

Can somebody help me with my bone tissue to remain intact during antigen retrieval for IHC staining. I have used subbed slides but It did not help me. Is there more better adhesive. Does Haupts Gelatin works better? I know this is a hundred year old question and hopefully this problem have

RE: [Histonet] Bone!

Thanks Kemlo... I thought of that, but was worried about over-fixation,- what do you think? Redward. -- In 10% neutral buffered formalin? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311

RE: [Histonet] Bone saw

IMEB also has a Bone Band Saw. http://www.imebinc.com/Item/BBS-82203.htm Jack Date: Thu, 21 May 2009 09:05:50 -0700 From: cb...@memorialcare.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone saw Good Morning, I was wondering if anyone can help me find a really

[Histonet] cloudy formalin in tissue processor

Has anyone had a problem with their formalin getting cloudy in their tissue processor? We have a VIP tissue processor and just recently started having problems with cloudy formalin. Any suggestions would be appreciated. I checked the archives and couldn't find any articles on this particular

RE: [Histonet] bone tissue

Haupts Gelatin works really well at a 1:1 ratio (Concentrate:50% EtOH). The only limitation is background staining from say a hematoxylin counterstain after the IHC. You can purchase the concentrate from Fisher (Haupts Fixative #785-71). Jack Date: Thu, 21 May 2009 10:12:23 -0500

RE: [Histonet] Bone!

I do not routinely perform decalcified bone preparations as I predominantly utilize undemineralized resin (MMA) applications, so maybe someone else can better respond to this question. However, I would say that you could store your bone in 70% EtOH until you are ready to process, but the

RE: [IHCRG] Re: [Histonet] HRP-labeled primary antibodies

I agree with Kim, if I was worried about a weak signal using abs directly conjugated to hrp I would just ignore the fact that they have the hrp and use a detection such as labeled polymer matched to the species of the primary ab just like I would without the direct hrp conjugation, there should

[Histonet] fume hood regulations

Fellow Histonetters, Does anyone have knowledge of guidelines or regulations on whether a fume hood must remain on 24/7? In a cost cutting measure a facility is considering turning off the fume hoods from 7pm to 5am. I have expressed my concern with the idea because of the huge health and

RE: [Histonet] cloudy formalin in tissue processor

Is your formalin picking up water from the air in the room? Check the humidity level in your room. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Christine I. Braaten Sent: Thu

Re: [Histonet] fume hood regulations

Hi, At the University of Washington we have been told to lower the sash all the way when the hood is not in use to save energy and money. I believe there have been several Universities that have done some cost analysis of this and have show large savings in money (one study stated $1500 per year

[Histonet] IHC question- freezing aliquots

Hello all! I have an antibody that has a 2 month expiration date. It is recommended that we aliquot, and freeze (-80) the antibody- per the vendor. How do you interpret: The CAP reg pertaining to expired antibody use: ANP .22432 ??? Are all immunohistochemical reagents used within

[Histonet] Dry ice - transportation

Are there any restrictions on the transportation of dry ice (and human tissue) in private vehicles? Our PAs cover a surgical center off-site and we need to transport frozen tissue back to the hospital for biobanking purposes. They drive back and forth in their own vehicles. Thanks. Richard

RE: [Histonet] IHC question- freezing aliquots

In a similar situation we have gotten documentation (e-mail) from the vendor stating the recommendation to freeze aliquots and to what degree that extends the shelf life. We haven't been queried on these particular antibodies by an inspector but our supervisor feels that this satisfies the CAP

[Histonet] Plant/insect histology

If anyone out in histonet land is doing above, could you please contact me by email directly? Thanks Luis /PRE html body br / This email message, including any attachments, is for the sole use of the intended recipient(s) and may

RE: [Histonet] Dry ice - transportation

Richard: There may be local regulations that could vary. Here you can buy dry ice in the grocery store (if you bring an appropriate container). The safety warnings associated with dry ice indicate (as you would expect) - transport in unsealed insulated container (Styrofoam chest), transport in

[Histonet] Adhesive for immunos

We have used 5% Tite-bond glue( from the Hardware store) on slides with great success. Just take a Kimwipe and wipe it evenly over the slide and it holds the tissue very well. Annette Featherstone, Supervisor of Pathology and Anatomical Sciences The University of Buffalo

RE: [Histonet] cloudy formalin in tissue processor

If you use NBF it will get cloudy when it mixes (in some step) with alcohol. The NBF has salts that are not soluble in alcohol and will produce cloudiness. You are getting your NBF mixed with alcohol. René J. --- On Thu, 5/21/09, Burton, Lynn lynn.bur...@illinois.gov wrote: From: Burton, Lynn

Re: [Histonet] cloudy formalin in tissue processor

This can result from not drying the retort or tissue tank after the clean cycle which will leave alcohol in the bottom.  Dry the tank beofre starting the next run.  Ocassionally I have even seen some xylene end up in a mixture in the tissue tank if it is not dried well.  Both of these will

[Histonet] Chris Stephenson is out of the office.

I will be out of the office starting 05/21/2009 and will not return until 05/26/2009. Please forward all urgent messages to Eric Halpern at ext. 5852 or eric.halp...@comphealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Re: Bone saw

Christine Bark asks about saws for cutting surgical pathology bone specimens like femoral heads. I posted something to Histonet about this fairly recently - here it is again. Stephen Peters, a pathologist in Hackensack NJ, describes for us a bone saw he's invented.

[Histonet] RE: [IHCRG] HRP-labeled primary antibodies

Kim, The direct IPX is the easiest method apart from a direct immunofluorescence. Very few steps: 1.Block endogenous enzyme 2.Block non-specific Ab binding 3.Put the HRP-conjugated Ab on (a an appropriate dilution, usually more concentrated than if you were using an ABC or Polymer

[Histonet] RE: [IHCRG] Re: CMV antibody

Interestingly, This data sheet does not seem to list the clone of the CMV monoclonal antibody, or am I misreading it? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead

RE: [Histonet] cloudy formalin in tissue processor

Possible carry over of xylene from the flush program? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag

[Histonet] RE: [IHCRG] Re: CMV antibody

Michael, I have used both the Dako (Clones CCH2 + DDG9) and the Novocastra products (Clones 2 and 6) and they both stain the same. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital

[Histonet] re:cloudy formalin

Are you certain that it is formalin and not paraformaldehyde? The latter will get cloudy. Also, is there any way that your clearing solvent might be making its way in to the formalin solution? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Antibodies for Alport syndrome

Hi All, Has anyone had experience doing IHC on FFPE tissues with Collagen Type IV subunit antibodies? These are Collagen Type IV alpha 1, alpha 3 and alpha 5 chains. They are used for the diagnosis of Alport syndrome and all antibodies are from KAMIYA BIOMEDICAL COMPANY, Seatle. At present,

[Histonet] Wholemount processing

Hello to all in histoland. My lab is looking to start processing whole prostate specimens and doing the wholemount process. Can anyone give me a starting point on how to proceed. I would need information on processing times, embedding and cutting equipment, staining information and the glass