Good Morning!
I was wondering who out there is using the Diff Quik II stain for H. pylori? I
am having a hard time finding where to buy it! Do you just buy the whole DQ kit
or do you just buy Diff Quik II solution? Any suggestions would be great!
Thanks!
Happy Friday!
Jessica Piche-Grocki,
I am having some difficulty getting the proper results from my reticulin
stain. I have been following and tweaking the procedure outlined in
Carson's book without any success. Could someone please forward me their
procedure that has all the kinks worked out?
Thanks,
Derek
--
Derek Papalegis H
Derek:
I am sending it to you under separate cover.
René J.
--- On Fri, 6/19/09, Derek Papalegis wrote:
From: Derek Papalegis
Subject: [Histonet] reticulin stain
To: histonet@lists.utsouthwestern.edu
Date: Friday, June 19, 2009, 9:11 AM
I am having some difficulty getting the proper results
I purchase Diff Quik Solution II in a 2.5L bottle from Cardinal Health Catalog
# B4132-12A. It's not something that they have in stock and it usually ships
from Dade Behring Inc. which is who makes it. It usually takes me a couple of
weeks to get it, but I have no problem receiving it.
Jodie
We have used mouse anti-Brdu from Becton Dickinson for years. It is still
available from BD BioSciences Cat. 347580. I know it is made in mouse but we
used an IgG1 secondary and always had good results. There may be some
cytoplasmic label, probably background, but you are interested in the nuc
Hello everyone,
I am looking for a cost of some of the Ventana reagents can anyone help?
Specifically:
LCS:
CC1:
CC2:
EZ Prep:
Reaction Buffer:
I am not currently a customer so I didn't want to phone Ventana directly
if I don't have to. Thanks
Tiana
This email and/or any documents in this tr
Good Morning HistoNetters- Good Used equipment for sale!
We have a Leica TP1050 bench model that is currently being used on a daily
basis. The 300 cassette capacity retort, will process with or without heat,
with or without pressure or vacuum. It has the reagent management system to
assist wi
`If you don't use their auto stainer probably you will not be able to use their
antibodies that, as far as I know, are "optimized" to be used in their
instrument in what they call "liquid cover slips" for room and high temperature.
René J.
--- On Fri, 6/19/09, Tiana Fountain wrote:
From: Tian
Hi Everyone!!
I am collecting spleen, brain, and spinal cord from virus infected mouse.
I will perfuse mouse with PBS. Do you think I need to use a new pair of
scissors and forceps for each tissue in each mouse and between each mouse?
Thanks,
Dz
The prices I have are on the list you sent. I couldn't find the CC2 price
quickly.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Tiana Fountain
Sent: Fri 6/19/2009 10:42 AM
To: histo
Ventana prices will vary dependednt upon your (not you per se, but rather your
instrument) consumption.
Here is what we pay:
LCS $74
CC1 $832
CC2 $895
EZprep (10X) $452
Reaction Buffer$65
If you need more info, please contact me
Thanks
Dusko Trajkovic
Pfizer Inc La Jolla
I have a pretty old microtome to donate. It is a Leitz 1512. I also have
some of those big blades that it uses. All you have to do is pay for the
shipping.
Cheers, Maggie
Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton Universi
I too will send you ours to your e-mail.
Matt Lunetta HT (ASCP)
Message: 13
Date: Fri, 19 Jun 2009 06:26:34 -0700 (PDT)
From: Rene J Buesa
Subject: [Histonet] reticulin stain
To: histonet@lists.utsouthwestern.edu, Derek Papalegis
Message-ID: <431812.3131...@web65714.mail.ac4.yahoo.com>
Cont
Happy Friday Everybody,
I have a user who has a precious sample and wants to embed in LR White, maybe.
I'm having him practice on something not precious first. My questions are:
1. He wants to do immunofluorescence and morphology on these nerve samples.
He is planning on perfusing in 2% PF
To stain Helicobacter, you need only the Diff-Quik II solution. This is a
brand name. I've used several generic equivalents and they work also.
Bob Richmond
Samurai Pathologist
Knoxville TN
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Hi,
I am trying to get a a pre-embedding immuno to work. I have tried
several different things (fixatives, incubation times, +/- detergent)
and it seems that definitely I need to have a detergent present. I used
0.05% Tween20, but the quality of the EM is really bad (it is impossible
to dis
Hi everyone,
Does anyone have a good technique for mounting bone tissue (rabbit
calvaria embedded in plastic) to coated slides? I am having problems
with wrinkles following the rolling of the sections onto the slides. I
would greatly appreciate any help you might be willing to offer. Thanks.
Jim
Does anyone use Formula 83 as their Clearant on a Peloris tissue processor?? If
so, have you had any problems?? Please let me know as we are experiencing
consistent problems with water in the Formula 83.
Thank you,
Ann
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Histonet
We buy Diff Quik II by the gallon directly from Dade.
You don't need the kit.
Judy Keller, HTL
South County Hospital
Wakefield, RI
From: histonet-boun...@lists.utsouthwestern.edu on behalf of
histonet-requ...@lists.utsouthwestern.edu
Sent: Fri 6/19/2009 1:01
Hi everyone,
I'm curious if anyone has tried using Superfrost plus gold slides, are
they any better than the regular superfrost plus? I'm dealing with
frozen lymph node on a Ventana discovery, I've tried a few different
fixing/drying combinations but I still have tissue lifting (not
completely bu
I had a group here who were having trouble with their cryo-sections sticking
to the slides. Their samples were rat brains. He found that the Gold Super
Frost Plus worked well with in-situ but not for immuno. At least that is the
comment he wrote down and put on our bulletin board in the lab. Most o
Good old fashioned gelatin subbing is very cheap & works great--I have never
seen tissue separate from a subbed slide! Susan
- Original Message -
From: "Peggy Bisher"
To: "Milne, Katy" ;
Sent: Friday, June 19, 2009 3:34 PM
Subject: Re: [Histonet] Superfrost plus gold slides vs super
I have lost the recipe for Acid Alcohol (used to decolorize H&E's).? Can
someone send it to me?
Thanks,
Ann
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But doesn't the gelatin's protein interfere with immuno results? That's why we
do not use glue, gelatin, or any adhesive on immuno cases.
Maria Katleba HT (ASCP), MS, MBA
Pathology Dept. Mgr
Queen of the Valley Medical Center
Pathology Laboratory
Napa Ca
-Original Message-
From: histonet
You did not say whether the tissues were prefixed with formalin or fresh
tissues, frozen then cryosectioned? If prefixed, cryoprotected, frozen and
cryosectioned, the Plus Gold should work. They are certainly more
expensive! A colleague uses Plus Gold for prefixed, cryoprotected frozen
sectio
Hello Histonetters:
I am having problems with tissue just disappearing in my lastest batches of
slides. I have been doing a lot of H&E's and did not have too many problems.
But I am cutting testis to use with a CD168 ab. Everytime after the 1mM EDTA
AR,the tissue disappears from the slide.
1% Nitric acid in 70% ethanbol.
Joe Saby
From: "thisis...@aol.com"
To: histonet@lists.utsouthwestern.edu
Sent: Friday, June 19, 2009 5:01:48 PM
Subject: [Histonet] Acid Alcohol
I have lost the recipe for Acid Alcohol (used to decolorize H&E's).? Can
someone
You did not say which slides you use, but you may want to try Thermo
Scientific EXCELL slides. The special coating resists the effects of high
pH EDTA antigen retrieval and heat. Hopefully, you are putting any
adhesives in the water-bath when using Plus Charge or any other coated
slides. The gel
We have used 0.5% to 1% HCl in 70% alcohoL but only for Harris regressive
hematoxylin staining. For progressive hematoxylins, and Gills, 1% acetic
acid was preferred.
Gayle M. Callis
HTL(ASCP)HT,MT
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-bo
Lynn
From: lynn...@hotmail.com
To: lynn...@hotmail.com
Subject: RE: [Histonet] xylene and frozen sections
Date: Thu, 18 Jun 2009 19:53:52 -0500
Is there any literature that says xylene will fade progressive staining H&E
(using Surgipath Harris Hematoxylin) over a period of years? Ou
1% cHCl in 70% ethanol (reagent alcohol), couple of seconds is plenty
Caroline
Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158
www.gladstone.ucsf.edu
On Jun 19, 2009, at 17:01, thisis...@aol
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