Tom, are the times you've listed benchmarks your facility has established for
it's operation? I'm not aware of any national benchmarks using those numbers
but want to be sure I haven't missed something.
Also, where is your frozen lab located in relation to the OR? Are you located
within the OR
We will be cutting frozen sections on sheep lung and I was wondering if
anyone has a method to produce good quality sections. Are you injecting
anything in the lung prior to sectioning?
Annette Featherstone
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There is also a histonetter that makes silicone array molds, you can buy them
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Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
Registration for the First Annual One Day Immunohistochemistry Forum for
Histotechs is now open. The event developed with Dr. Richard Cartun and
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Hi Histonetters,
I was given 3 antibodies that were worked up previously with 1% glycine for 30
mins. prior to antibody incubation. I have not come across this before and was
wondering if anyone knows the purpose of its use. Is it a blocking reagent. The
antibodies that I am using it on are
Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical
University of South Carolina
Charleston SC asks about tracking turnaround time of frozen sections (note
that not every intraoperative consultation requires a frozen section).
The few services I've worked on that attempted to
Is anyone working with MCP-1 Armenian hamster, from Biolegend? This is for
FFPE tissue. The secondary is anti-Syrian hamster. Any help would be
appreciated.
Annette Featherstone
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hi every one,
I am using Labvision HRP polymer secondary antibody with
DAB chromogen, funny thing is that i have exhausted the DAB substrate buffer.
so i tried with PBS 7.4 and h2o2 and chromogen and i found out that nothing
worked. can anyone tell me a protocol or recipe
Hi all
i read such figures in Cajal's books..I guess he is not using specific
degenerative sliver staining, as Natua developed the method 50 years later.
I wonder anyone can send me the protocol for such kind of silver staining -
there are so many ways of Golgi staining - to reveal post-injury
Hi,
Wondering if anyone knows of a Beta-Amyloid antibody, for use on FFPE
brain sections, that doesn't need formic acid pretreatment?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
No, it's still the same. 20 minutes is the CAP TAT.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildrens.org
Thanks Dr. Richmond. CAP's turnaround time requirement for frozen sections is
unchanged.
My question was prompted by the fact that we have an individual internal to our
organization pushing for measuring turnaround from time of order to time result
is issued, which muddies the water, at least
Vinnie,
We have always measured from the time the specimen is received in
Pathology. Our frozen section room is adjacent to the OR.
Victor
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
The hospital where I work just opened a new lab. The histology department is
now combined with cytology and is made up of me, a second histotech, a cytology
prep tech, and our cytotech supervisor. We didn't have a hood in the old lab,
so we did our manual special stains on a workbench with
I think a fume hood and a safety shower should be standard laboratory
fixtures.
I have a fume hood. I use it for manual coverslipping. I require my
students to do the same. OSHA says that exposure to xylene fumes can cause
liver damage. There is also a persistent rumor that repeated
Vinnie,
I am very interested in this discussion about tracking from time of order to
completion. I am particularly interested how or why your institution decided to
develop a new tracking parameter that neither CAP or JCAHO require. What drives
this decision? What is the specific problem and
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