Pale liver means the blood has been removed from liver, not necessarily any
other tissues of interest.
For brain perfusion, many people will close descending atery.
The lung filling up of liquid is because you did not insert needle into the
main artery. It is small, but can be done with proper
let me say like this
during the overdose injection, look into the animals' eyes.
take their soul away. then the pre-mortem perfusion is humanized.
2009-07-01
TF
发件人： Chana de Wolf
发送时间： 2009-07-01 09:30:01
收件人： Ingles Claire
抄送： histonet
主题： Re: [Histonet] Good Perfusion...
I think many, many labs perfuse animals under deep anesthesia. Notice the word
DEEP. It is hard to judge whether the animal can recover. But we know not
because it is an overdose injection.
Then, when you write papers up, you never use Deep anesthesia, you use
sacrifice. That is some back
Good Morning,
I have not received any replies to my proposed question regarding the
following question. I don't know if the histotech's that have the
answers were on vacation, but I sure would like any information.
Below is the original e-mail:
I need your assistance on a on-going
Hi Histonetters - do any of you use easin in the processing alcohols in
order to better visualize small bipsies and if so how much eosin to alcohol
do you use and in which alcohol?
Thank you in advance for your help.
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Hey everyone...just thought I'd add an official word from the authorities
in this debate...as I'd been taught or somehow led to believe otherwise:
:-)
Forwarded Message
Date: Wednesday, July 01, 2009 11:19 AM -0400
From: IACUC ia...@research.buffalo.edu
To: Merced M
It is also considered incorrect to use the word sacrifice, implying some type
of ceremony, religious or otherwise. Animal rights fanatics have a hey day
with this word. Euthanasia is more correct.
Gayle M. Callis
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
hI, i AM using formalin-free Zince fixative made from Zinc salt Tris buffer.
Then I made frozen sections after dehydration in 30% sucrose...
No CD31+ staining...
I also tried shock frozen, acetone fixed tissue, the antibody works in this
condition.
2009-07-02
TF
发件人： gayle callis
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we received a letter from the lab that we send
our prostate biopsies to saying that it was undesirable
Folks,
I inherited some nice new metal cabinets (Sakura Tissue Tek) brand, for
holding glass microscope slides. The drawers are missing the inserts that
make so you can line up two rows of slides side by side, I am wondering
where I could buy those.
Thank you,
Patsy
Patsy Ruegg,
We use eosin on the gross bench. We keep a bottle of concentrated Eosin
and Hematoxylin on the bench with an eyedropper. The eosin is for
endoscopic biopsies and the hematoxylin is for prostate biopsies. Neither
has never interfered with IHC staining.
Debbie M. Boyd, HT(ASCP),Chief
We use about the same amount or eosin in the first 100% alcohol and also do
immunos on the biopsies and have no problem. We do all GI biopsies though not
prostate. I'm not sure why the eosin would interfere with the immuno staining
since it disappears during the hydration and retrieval
Jennifer, is it possible that the lab asking you to use cobalt blue is using a
fluorescence technique on the prostates? We've used eosin for years and not
seen any impact on IHC staining for light microscopy.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of
I think they are probably getting weak unwanted staining with P504s/AMACR
antibody on benign glands on their PIN4s.
Since this antibody is usually pink/red, it sounds like someone might have
decided that the unwanted color came from eosin in the processor, but this
would be the wrong conclusion.
Hi Everyone,
I'm looking to see if anyone has used EDC fixation with formalin, if so do you
EDC fix first followed by formalin or formalin fix followed by EDC. I would
love to have some feedback on this.
Regards,
Sharon
I have never heard of eosin interfering with IHC, as a matter of fact, I did
use it regularly and never had a problem with the IHC.
Perhaps there was another cause for the problems with the IHC.
rené J.
--- On Wed, 7/1/09, Jennifer Johnson jmjohnso...@hotmail.com wrote:
From: Jennifer Johnson
Are the prostate biopsies, fixed in formalin or Bouins?
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
From: histonet-boun...@lists.utsouthwestern.edu
We have a Symphony and my techs absolutely love this instrument. It is very
easy to use and the stain/reagent maintenance is so simple. No more changing
and rotating solutions. The staining quality is excellent and Ventana's
customer service is great. I would definitely recommend the Symphony.
Hi Akemi,
The only time I remember anyone ever saying California histotechs had to be
certified was when you posted something a while back. You didn't give any
information about from where this information came. Where'd you hear this
rumor?
Mark Tarango
On Wed, Jul 1, 2009 at 6:14 AM, Akemi
This rumor has gone as far as my classroom. Our teacher has mentioned it for
many years as a possibility. I have never seen or heard of any official
source.
Sent from my Verizon Wireless BlackBerry
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I think I remember someone saying once that this was part of a failed piece
of legislation. I thought I'd read it on histonet, but I couldn't find an
old post saying that.
Mark Tarango
On Wed, Jul 1, 2009 at 2:57 PM, theci...@yahoo.com wrote:
This rumor has gone as far as my classroom. Our
That sound you hear is George Orwell turning over in his grave.
Bob Richmond
Samurai Pathologist
Knoxville TN
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I have heard similar responses to yours and I think there is agreement on the
quality of the instrument, but what are you paying per slide and how did you
justify the cost? In your cost justification, were you able to convert to
dollars any of the quality improvements and can you share that
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