Michael,
Ditto, very well stated. I too believe that our industry is under appreciated.
Many new grads of today find a two year degree demeaning and wouldn't consider
HT because of it. I don't understand how some professions like pharmacy &
physical therapy gain respect and grow to create 5 yr,
Igor.
A few years back I used Dako V9 mouse anti human vimentin to label human grafts
in a mouse background. Tested it first on several mouse tissues and got no
reactivity, compared to very strong reactivity in a variety of cell types in
human tissue, and so am sure that it is human specific c
Hey everyone, I'm actually a early 20s histotech without a certification and
run my own routine histo lab.
*waits for "young whiper snapper" remark*
But I am in the process of completing my education.
In all seriousness though I have to agree many out there are looking for cheap
labor - so I t
Colleen,
One of my points of frustration is that we have never been on the same level as
MT's and MLT's. I feel that part of this is because of our level of education
and requirements. After seeing little changes over the years it is frustrating.
I'm sure I'm not the only one who feels thi
Dear Histonetters,
I have a question about alternatives to formalin fixation and fine changes
in tissue structure.
We have been obtaining formalin fixed human skin and fat samples from
several companies. We use stereological methods to make tissue measurements
such as dermal thickness and adipose
To Ron Martinhistology IS BOTH A PROFESSION AND A
CAREER! You need a new job!
Colleen Forster HT(ASCP)QIHC
Wahlberg, Nikki wrote:
I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen a
Just my opinion Nikki. Sorry you don't agree but at least I respect your
opinion.
- Original Message -
From: "Nikki Wahlberg"
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, July 14, 2009 7:02:48 PM GMT -05:00 US/Canada Eastern
Subject: RE: [Histonet] What percent of HTL's d
I would just like to add that in my opinion it is people who make statements
like the one below that are holding our field back from being seen as a career.
The hospitals as well as the doctors are also to blame. I am very proud to
have a B.S. and A.S.S. degree and an HTL certification. I
Thought I would throw my 2 cents out there, too. I received my HTL back in
the early 80's (hate to think how old that makes me!), I already had a BS
when I went to histotech school. I have found that it has made a difference
in my career to have the HTL. When I have worked in hospitals, I was on th
Too bad someone doesn't do a 'survey' on this site where the question can be
answered with real data...
MK
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Tuesday, July 14, 2009 2:37 PM
To
We are NOT using Copath however...
I think the only time you have to separate Cytology from Histology is when you
are dealing with GYNs. Otherwise, you can and should use the S09 (or what ever
you give Pathology Surgicals). Why would anyone want to have two separate
reports on one specimen?
I wouldn't say that histology is a dead end job, I mean, I jumped to a PA
and I know that I couldn't have passed that exam without my histology
experience. The idea of adding an HTL category was initially a good one.
Trying to bring us up to the MT and CT levels, but honestly, I think it fell
s
Mike,
I couldn't agree with you more. I'm in the same position as you. In the Boston,
Mass. area people are taken right off the street and work for a year as a lab
assistant then promoted to a tech in training. Most have a hs diploma, no
ambition and expect good pay for bad work and poor w
"polycyclic aromatic flourescent compounds that in high concentrations"
I wouldn't think the 3 mls of eosin dropped in the last 95%
alcohol could be considered "high concentration" but that's what keeps
Histonet entertaining; I learn something new every day.
-Original Message-
From
I am with you Joyce, still going strong for 47 years and with no plans to
retire, actually working my day job and a part time. I may not make it to 100
like you, but they will roll me out feet first. There are more of us around
than people know. Scary huh?
Shirley
-Original Message-
F
MB,
I think your prediction, sadly, is true and that it is already
happening.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[
HI all
I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the
early 90s and I guess I was misguided because I thought it would open more
doors for me than just an HT. I was sadly mistaken. After I passed my test
I waited 9 months for a raise and promotion (which was just a gre
Tom,
I missed being grandfathered in by a matter of months and am about 10
years away from retirement. I personally know of several HTLs that
don't have Bachelor degrees that are around my same age. So in my part
of the Universe, non-degreed HTLs are slightly more prevalent than a
needle in a ha
Honey... You are a mere child! There are some of us that have been in
the business for 40+ years. I missed the grandfather approach by 7 mo -
time that I didn't work moving from place to place with my military
ex-husband.
But I did finally get the degree and do the exam. But we're still
around.
Hello HistoNetters
We are selling our Ventana Benchmark XT IHC stainer. If anyone is
interested, please see the ad on our web site.
http://www.sarapath.com/equipment4sale/ I would be happy to answer any
questions, please feel free to email or contact me directly.
Thank You
Brian Brant
Hi Steve,
I've got no statistics to offer you...just an observation. I would say
that finding an HTL, without a Bachelor's degree is akin to the
proverbial needle in a haystack. Anyone that obtained their HTL,
if/when they could be grandfathered in, is likely to be retired or close
to it. First
Dear Histonetters!
I am wondering if anyone can possibly advise good antibodies for HUMAN anti
alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse
stroma and in the past had a lot of cross reactivity and background issues.
Does anyone know good antibodies or a clone, or has
We have recently used MACO ORT25c film in unperforated 35mm format in
transmission electron microscopy. It is touted as a replacement for Kodak
Technical Pan Film. After dessicating the film, it demonstrates a separation
of the emulsion from the film base primarily at the cut ends of film st
Hi All,
I am thinking of switching the paraffin we are using for our processor and for
embedding. We are using the Fisherbrand Paraplast Plus which contains DMSO for
both the processor and for embedding.
I used to use Suripath's paraplast and loved it. I would like to know what
everyone in h
The survivin antibody that I have used and gotten good results with was Cell
Signaling #2803 at concentrations between 1:30-1:50, using an autostainer
with pretty standard protocols.
Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Transla
Hello,
Are there any sites that are using CoPath with one number wheel for
accessioning Cytology and Histology cases together?
Right now, we have two separate departments with Cytology ordering under
"C09-" and Histology ordering under "S09-" and we have have totally
separate specimen classes.
We also use and like the Artisan.
Joe Galbraith
University of Iowa
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Tuesday, July 14, 2009 9:42 AM
To: kristen arvidson; histonet
Subject:
Artisan does it for me - good stains, good backup, few if any delivery
delays, application specialists 'on tap' via email direct fromDako Denmark -
AND the machine does a MEAN Warthin Starry!!!
Annie in Arabia
2009/7/14 kristen arvidson
> What stainer is everyone liking the best?
>
>
>
> ___
A couple of weeks ago I posted the message below on the histonet and all of you
responded that it shouldn't matter so I have finally gotten a reply from the
company we send our prostate biopsies off to and below is their response. So
now you know the rest of the story!
We have used Eosin i
Is there anyone using the Sakura DRS Stainer for H&E Staining?? I am presently
trying to validate an H&E stain using the following reagents?:?
?Hematoxylin? - Cardinal Health, 7211
Clarifier - Cardinal Health
Bluing reagent - Cardinal Health
Eosin Y - Cardinal Health
I am decerating the slides f
Hi Jerry,
I would never try to persuade anyone. I'm no smarter than the next lab worker
trying to make sense of this and science biology in general. But this is how I
see TUNEL and FFPE and after all the years I'm happy with things.
I have not noticed false positives at all when looking
The Artisan (DAKO) is the best.
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Tuesday, July 14, 2009 9:13 AM
To:
Hello!
I am trying to stain dendritic cells (DCs) - from the immune system (not the
brain) - in formalin/paraffin sections. I've heard that fascin is a commonly
used marker but I don't think it's very specific - I see some endothelial
cell staining too. Looking online, I've also found other poss
What stainer is everyone liking the best?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hallo Everyone,
For those of you who have used this antibody on FFPE human breast tissue, can
you provide me with a protocol and antibody information? I have tried
antibodies from Cell Signaling, Spring and two from Biocare, however I am not
getting the results that are the same as in the publ
Hi everbody,
I'm currently looking for an antibody that stains myelin in mouse
brains (no animals with EAE). Does anyone have experences with
antibodies against MBP, MAG or other myelin proteins in mouse brains
and can give me a suggestion, which one is the best to order for
paraffin embed
36 matches
Mail list logo
