[Histonet] Histology Job Opening

To All We have a Histology Job Opening Day Shift 730a to 4 pm. Please call or send resume. Thanks Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email almin...@tuhs.temple.edu This electronic message is intended to be for

[Histonet] histology for kids

Hello All, My company is hosting an in-house science awareness day for local grade-school students.  I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).  Does anyone know of any house-hold dyes (grape

[Histonet] Formula 83

Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing.

RE: [Histonet] Formula 83

Phyllis, I have used Formula 83 for several years and have no problem with it. I do use it just like xylene. As for how frequently you need to change it on the processor really depends on your volume of processing. I didn't have to change the frequency when I switched from xylene to Formula

Re: [Histonet] histology for kids

Try saffron, in reality it is an acceptable stain for regular grown-up histology as well. René J. --- On Wed, 7/22/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] histology for kids To: Histonet histonet@lists.utsouthwestern.edu Date:

[Histonet] Histotech II position open.

Hello all. I would like to inform the histotech community of an opening for a Histotech II position. We are looking for someone with experience who is willing to learn. Renal, Muscle, and IHC experience is a definite plus, but we are willing to teach if the candidate is motivated. Please apply

Re: [Histonet] histology for kids

Maybe you could use a sponge (representing tissue) soaked in water and demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled (precise cutting) explaining the water is taken out of the cells and replaced with wax. Rene J Buesa rjbu...@yahoo.com 07/22/2009 07:15 Try saffron, in

Re: [Histonet] histology for kids

red food colouring, green and blue too as well as beetroot and grape juice and saffron could be tried in advance on the slides - just to see how colours can be combined you will only know by testing in advance - or you may end up with a brown sludge covering the whole section look at hairs and

[Histonet] Slide dryers

Hi All. We are re-working some of our processes (fun!). We are trying to get more LEAN and shave some time off of our processes here there. One thing I am looking at is the small forced air slide dryers. I have seen some good comments on the mopec roto-dry. Has anyone tried the dryer from EMS?

[Histonet] Re: Formula 83

We discussed formula 83 earlier this year. http://lists.utsouthwestern.edu/pipermail/histonet/2009-January/041976.html Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Histology Supervisor needed in Washington State

Hello Histonetters! I have a new position I want to tell you about. I am currently working with a hospital located in Washington State who is in need of a histology supervisor. ASCP HT, Hands -on histology and supervisory experience is required. My client offers a great salary and benefits and

RE: [Histonet] histology for kids

Try this website for ideas... http://www.mnmicroscopy.org/ProjectMicro/Welcome.html Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

[Histonet] Start Up Lab

And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histology for kids

I did tonsils one time and since most of them have had theirs removed, they were very interested. We also have autopsy tissue, brains, lung, liver, heart, in jars of formalin which are always a hit for them to see but not touch! Good Luck, it will be so much fun watching their faces.

RE: [Histonet] histology for kids

Kim, Contact Mary McCann who is ProjectMICRO's coordinator for NESM (New England Society for Microscopy). NESM took on ProjectMICRO as a pet project a number of years back. We put together 3 kits of materials (microscopes and consumables). Two of the kits are in constant use in Vermont and

RE: [Histonet] histology for kids

A number of dyes used in histology are also approved for use in foods. These include: Brilliant Blue (FDC Blue #1) Fast Green FCF (FDC Green #3) Erythrosin (FDC Red #3) Tartrazine (FDC Yellow #5) Carmine ___ Histonet mailing list

[Histonet] Re: Citadel 2000 Tissue Processor

Hello, will everyone who's used/using the Citadel 2000 Tissue Processor please share with me your opinions, pros/cons and etc ASAP? Thanks! Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] histology for kids

If you ripen it with air or sodium iodate, alum hematoxylin is quite safe. FDC green #3 is food grade fast green FCF, an excellent stain for collagen. FDC yellow #5 is tartrazine, a plasma stain. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] histology for kids

One more thought, NSH has a small paper pamphlet out call Histology (hiss TOL-o-je) which has pictures, puzzles, anatomy charts (simple) to help teach kids. Written by Judy Stasko, CLT and Jan Gardiner, BAAS, HT(ASCP). Kathleen Boozer booze...@ah.org 07/22/2009 07:46 Maybe you could use a

[Histonet] More quality stuff

Hi- As most of you have gathered I am working on a large quality project.  I have made a lot of progress.  Currently, my focus is error prevention.  I have read up on tools such as six sigma and FMEA.  My question is how do you guys prevent errors?  I have learned that you cannot always count

Re: [Histonet] More quality stuff

We have had tremendous success with setting up checkpoints at each step in tissue processing (cradle to grave). Each checkpoint has certain responsibilities to identify, double-check, and assure accuracy. I keep track of discrepancy errors at each checkpoint and determine if personnel need to

Re: [Histonet] More quality stuff

Hi Kathy, Do you have a sample form you would be willing to share? thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Kathleen Boozer

Re: [Histonet] histology for kids

Thanks to everyone that emailed me, I received so many ideas!  I will let you all know what I end up donig. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Kim Merriam kmerriam2...@yahoo.com To: Histonet histonet@lists.utsouthwestern.edu Sent:

[Histonet] Start Up Lab

I would add an eye wash station and a chemical spill kit to the list. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007

[Histonet] Re: Chromium Trioxide

Deanne Knutson, Anatomic Pathology Supervisor, St. Alexius Medical Center, Bismarck, North Dakota asks: We use Chromium Trioxide in our GMS stain, and the cost has escalated tremendously. Does anyone use a substitute for this chemical? I am curious what others are using for their GMS stain. We

Re: [Histonet] Re: Chromium Trioxide

Bob is correct that there is no satisfactory substitute for chromic acid in the GMS procedure.  We found that it takes 1 hour at 56-60 degrees C in 1% periodic acid to equal the usual oxidation with chromic acid, and even then you will probable get more silver staining of the connective tissue

RE: [Histonet] Start Up Lab

In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot.

RE: [Histonet] Start Up Lab

(lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of

[Histonet] Re: Citadel Tissue Processor

Hi Atoska, We do only research and have low volume. We purchased a second hand one and used it for about a year. It works and does the job. It is much better than having to process manually but: The alcohols evaporate like crazy! You loose a lot. It must be in a hood. There is no heating nor

RE: [Histonet] Start Up Lab

Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM

Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To:

[Histonet] 100 micron sections

Hi, I am being asked to section paraffin embedded fat at 100 microns. I haven't sectioned anything in paraffin thicker than 20 microns. What are the tricks to get it to unroll in the water bath? -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of

[Histonet] Chromium trioxide

You can use periodic acid to oxidize your tissues as you would for the PAMS stain. However, this is not the optimal solution when staining fungi. 5% chromium trioxide used for the GMS can be reused. Since you are doing the stains manually, as many of us do, this works particularly well.