Dear Sally and Histonet,
Fish can be fun. If they are young the bones are soft enough to cut without
decal. Or if you can fix them in an acidic fixative like Bouin's the bones
will be soft enough to not give trouble.
We do median sagittal sections and process and embed both sides.
They look
The file is attached with.
M. Tahseen,
Supervisor Histopathology
SKMCHRC, Lahore,
Pakistan
Good Day Histonetters,
Does anyone have a general orientation checklist for histology for new
hires that they would be willing to share with me? Thanks in advance for
your help, Amy
Amy Self
I love our Xpress! Our lab is certainly not typical...we are very
different from a routine hospital lab. We are an infectious diseases
group so primarily receive liver tissue (both autopsy and biopsy), skin
biopsies, kidney, lung, GI...pretty much everything other than
breast, uterus,
I agree 100%. Beecher is the worst vendor I have ever dealt with...and
unfortunately for Tissue array equipmentyour choices are pretty much
Beecher, Beeecher or Beecher..Chemicon had one, at a time...but, Beecher bought
it from them. Isn't there some laws about monopolies? They seem to have
Hi,
I would like suggestions on how to eliminate or decrease the effects of
extended storage of muscle bx's in a -70°C freezer. We do approx. 150 muscle
bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's
indefinitely, (we have them all from the 80's on). We are asked
Hello All,
I will be running a study upcoming and we have decided not to run FFPE
as we normally do and have decided to use flash frozen. My big concern
is what is the proper way to fix all of these SKIN samples to use for
IHC. Any recommendations as to if prefix the samples in sucrose and
We have a new project pending with mouse eyes. The lens must remain intact.
What is the best method to process, embed and cut them? Paraffin or glycol
methacrylate?
Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W.
Room 312
Montreal, QC, Canada
Anyone out there in Histoland have any experience with Thermo slide mate? We
are looking into purchasing a few. Thanks for any input, Janice
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Histonet mailing list
Histonet@lists.utsouthwestern.edu
I used to cut entire eyes of fish and lizards embedded in paraffin with great
results. The only thing is that on those (many years ago) days I used clearing
with Canada balsam followed by a short wash in benzene (not very safe) before
the paraffin infiltration.
René J.
--- On Wed, 7/29/09,
We have two units that we bought from Accuplace when it was called the
PSLIM. We had a fair amount of problems with them and I was ready to
return them, but Accuplace sent someone out to service them, and they
are working really well. I've heard that when Fisher took over the
slide printer, they
Sharon,
We process quite a few samples as well and I did them at Hopkins with a similar
time frame for the old tissue. We did not experience the problems you are
referencing when we pulled the cases out from storage. What is the exact type
of artifact that you are seeing? Is it freeze/thawing
Our pathologist would like us to set up a procedure for cytologies
(urines, in particular) that will NOT lyse red blood cells. Does
anybody know of a transport/preservation medium for urine cytologies
that does not lyse RBC's? And is anybody using it with ThinPrep? We
are currently using
Do they also want undetached retina? If so, do not use NBF to fix.
Paula Pierce, BA, AAS, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway Ave.
Moore, OK 73160
405-759-3953
Specializing in whole eye histology. All species.
From: Jo-Ann Bader,
Have also been doing them since 1982know what you mean about the artifact.
We store in Bell-Art containers (availavle thru Fisher)..We have found no
others to have the ability to storewithout cracking for so long.
We do place about 100 ul of water in the storage container...and place it in
Janice,
I know this well! It is a terrible item. This was originally sold under
AccuPlace and it barely worked, but showed very nice at various meetings under
a very controlled environment. In fact it worked so well, that the nice people
at Thermo decided to purchase this from AccuPlace and
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68,
Containers: Fisher # NC9283918 $32.90/6.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To:
Hello,
I am having problems staining my FFPE mouse brains for macrophages. I started
out with F4/80
marker, but have now moved to CD68. My positive controls (mouse spleen and
liver) work fine, but
I cannot get anything to stain in my sample tissues. I have see through H and
E staining that
Has anyone had any luck with Glycophorin A antibody (Cell Marque) used
on paraffin-embedded (Formical decalcified) bone marrow biopsies?
Justin Peters, HTL (ASCP)
IHC Supervisor
Bostwick Laboratories(tm)
For Absolute Confidence(r)
4355 Innslake Drive
Glen Allen, Virginia 23060
Phone:
We are a Mohs clinic and I had done a study on the feasability of IHC on frozen
skin. I cut slides as normal then fixed in acetone before using Biocare's Mach
3 kit (Alk. phos. with Red chromogen) with predilute antibodies. They mostly
turned out well, except for the S100. I don't believe that
This number did not work for me at the Fisher website. Is it current?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barone, Carol
Sent: Wednesday, July 29, 2009 2:19 PM
To:
Does anyone have any experience with the Leica IPC cassette labeler and IPS
slide labeler or the Sukura Tissue Tek Auto-write systems. We are setting
up a new lab and need labelers that will interface with windows operating
systems and do not have these experiences.
Best regards,
Patsy
I will be out of the office starting 07/29/2009 and will not return until
08/03/2009.
I will out of the office on Vacation starting Monday 7/29 and will return back
to work on Monday 8/3 in my absence if you need immediated attention please
contact Kiersten Ferreira the Operations Manager at
BUT...I have a small lab and can't justify a more expensive printer so
we purchased this printer - in fact we probably purchased the last one
that AccuPlace sold before Thermo got it.
I like it a lot and even though we have returned a couple of them we
have no complaints. It does a nice job.
Dear all,
Might anyone be able to offer their advice on the best method to retrieve
antigens on paraffin embedded skin sections? I am immunostaining for
epithelial cytokeratins, and I have block-to-block variation in the
quality of the staining - some block give consistently good staining
try this:
BD CytoRich™ Blue Preservative*
General Purpose Cell Preservative.
Excellent for Urine and non-hemolytic samples.
Recommended for use as a general cytology preservative
Gene
-Original Message-
From: Hayes, Tina J. tina.ha...@va.gov
To:
Sharon,
Your answer is probably in the following article in the Journal of
Histotechnology (Volume 32, number 2, June 2009):
Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic
Activity and Morphology
Authors: Iren Horkayne-Szakaly, Glenn D. Sandberg, Joren Keylock,
I guess I'm confused. If pricing is an issue, why not go with a Zebra
printer that prints labels. I wholeheartedly agree that printing directly
on slides is a preferred solution, but the up front cost is a killer. I
wouldn't blame a lab for going with slide LABELS.
Frankly, if it was me, I'd
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