Re: [Histonet] Stain Disposal

Sally, the Hazardous Materials in the Histopathology Laboratory Regulations,, Risks, Handling and Disposal is a nice reference book to have. It can be purchased from Anatech LTD 1-800-ANATECH Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University 1800 Denison Ave

RE: [Histonet] Freezing in Hexane

Hi Dorothy, I've not used hexane. I think 2-methly butane is more commonly used if you are, as I suspect, snap freezing in a solvent chilled in liquid nitrogen. The solvent just needs to have a low freezing point. 2-methyl butane freezes at about - 150 degrees C. I do not know if hexane has a

Re: [Histonet] optimal IHC thickness

I always used 3-4µm and prefer HIER over enzymes. René J. --- On Thu, 8/13/09, Katelin Lester kate...@cuttingedgehistology.com wrote: From: Katelin Lester kate...@cuttingedgehistology.com Subject: [Histonet] optimal IHC thickness To: histonet@lists.utsouthwestern.edu Date: Thursday, August 13,

[Histonet] advice about bone section please

Hi all, I'm planning to do paraffin embed section on mouse joint (E12~P10) to detect the GFP-tagged protein expression. Since bone has autofluorescence, I'm wondering whether IHC would be a good choice for postnatal bone, and can I do immunofluorescence on joint in E12~16 mouse without having

[Histonet] Picric Acid

Hello everyone! I would like to know what all of you are doing now that picric acid is so hard to get? We use it for bouin's, trichrome and gram stain. I know we can buy these already made up, but we wanted to know if there might be other alternatives. I'll be off tomorrow (state

[Histonet] Re: Freezing in Hexane

Dorothy Murphy Traczyk at Murphy-Traczyk Associates in Point Pleasant, NJ asks about using hexane for snap-freezing. I wouldn't want any of the hexanes (there are several isomers) or 2-methylbutane around my lab because of the explosion hazard if I could avoid it. Look up 3M Novec Engineered

[Histonet] Specimen Storage

I work for a research company and am looking at ideas for storage of specimens. We currently use Bitran bags to keep the extra parts with some formalin and then heat seal them in the Kapak heat seal bags. The Bitran bags are getting so expensive and tend to leak. Does anyone have a better

[Histonet] X-gal staining

Hello, I was wondering whether anyone might advise me on X-gal staining. I am looking at the expression of LacZ knocked into the locus of our gene of interest. We cryoembed, take sections and stain for beta-galactosidase activity using X-gal. The blue stain is often punctuate and noticeably

Re: [Histonet] Specimen Storage

We used to store them directly in the sealed Kapak pouches. I believe there are some for home commercial alternatives, although probably for frozen storage. Worth exploring out of the lab alternatives. René J. --- On Thu, 8/13/09, Susan Raibley sraib...@yahoo.com wrote: From: Susan Raibley

[Histonet] Laura Miller is on vacation.

I will be out of the office starting 08/13/2009 and will not return until 08/17/2009. I will respond to your email on Monday. Thanks! __ This email has been scanned by the MessageLabs Email Security System. For more

[Histonet] Negative Staining Positive

Has anyone noticed their negative mouse having some positive staining using the Ventana Benchmark XT? We use the iview detection. Any ideas? Thank you. -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States ___ Histonet mailing list

[Histonet] NSH Meeting Hotels

Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium.  I went to the NSH website and looked under the Hotel Travel icon and saw that the only hotel that wasn't Sold Out was the Double Tree.  I called and they never