Adequacy is carried out by the Biomedical Scientist usually in the UK. I
make a couple of air dried per passage and stain Diff Quick; you can
then give an indication of adequacy within 5 min or so. Usually if the
FNA is performed properly you don't get much material;; for example
breast and LN's
I recently had a discussion with one of my coworkers about
the need/requirement for blocking of endoegnous biotin whenever an
avidin-biotin detection system is used, and I was hoping that the IHC
experts on the histonet might be able to provide us with some feeback. Its
been my understanding that
We use biotin blocking only with certain tissues, i.e. liver, kidney,
GI, or diseases, i.e. oncocytomas.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From:
I went to archives to look for this but I didn't get a definite answer to my
specific question. I am using Biocare's Prostate Cocktail - 2X (CK5 + CK14 +
p63) and Biocare's P504S-2X on the Ventana platform using UltraView DAB and
Ultra View RED kits. To code this procedure, is it 88342 X 3 or
that's funny~~
just keep everything tight and unmovable.
also, we use anti-roll guide to keep the sections flat
so, sometimes there is a bit OCT attached to the blade/glass/anti-roll guide or
surrounding the tissue.
clean it.
have you tried to cut at a higher temperature? the tissue could be
Its three... Two different chromagens, 1 nuclear and two cytoplasmic abs, which
cannot be differentiated. You can bill for what can be separately identified.
Best, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
Dear All,
We are preparing harris heamtoxylin for HE in our laboratory. some
time we get intense nuclear staining some time light weak.Does anybody know how
to control a consistency in the strength .
Aazath
Technical Officer
Apollo Hospitals
India
I have been trying perform immunohistochemisty on formalin-fixed
paraffin-embedded skin samples with a vitamin D receptor antibody with
no luck. I'm using the Abnova antibody VDR monoclonal, clone 2F4
(catalog #H7421-M02). Has anyone used this antibody, even on
Your understanding is correct, imho.
I add a no primary control on my stABCpx-DAB run whenever I am testing new
tissues and assess any positivity that could be due to endogenous biotin.
You will quickly build up a knowledge of those tissues that automatically
require biotin - blocking. ( eg
Hi histonet,
I am back with a problem I've been having with my IHC slides. We had a PM
recently and had thought that would solve this problem, but as I do more and
more slides, the problem remains. I am seeing marginated staining at the
edge of the tissue on the controls as well as the patient
The hematoxylin has to be ripen, either naturally (it takes long time) or with
an oxidizer.
René J.
--- On Tue, 9/1/09, Aazath Raj aaz...@hotmail.com wrote:
From: Aazath Raj aaz...@hotmail.com
Subject: [Histonet] HE preparation
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 1,
Hi,
Can anyone on the Histonet recommend a good book of Neuropathology
techniques/methods for use in a Neuropathology Lab. Need more up to
date reference material.
Thanks
Sharon Allen
Neuropathology Lab
HSC - Wpg
sal...@hsc.mb.ca
This email and/or any documents in this transmission is
Hi,
Can anyone out there recommend the best way to stain mouse mast cells in the
kidney(toluidine blue/or berberine sulfate) with a protocol that produces
cconsistent results?
Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys.
Does anyone know of a company that
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