Good Morning all- I am having issues getting ink to stick to the tissue
surface, the black and blue that we are using are fine we have tried several
different companies and even use a ink stay acetic acid spray on the tissue.
My new pathologist has talked about dipping the tissue after it is
My Docs use acetone.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher,
September
Sent: Wednesday, September 02, 2009 7:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ink issues
Good Morning,
We dip the inked tissue in a container of Acetone. We use the 7-dye kit from
Bradley Products, Inc.
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
-Original
We use acetic acid - 3-5% solution (vinegar). j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Amspacher, September
Sent: Wednesday, September 02, 2009 07:03
To: histonet@lists.utsouthwestern.edu
Subject:
the good ole days for sure - purple ceilings, purple walls, purple
people And cigarette ashes in the trash and food on the counter...
Is it Friday yet?!!!
J:)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
For the first time here, we have Urologists using the robotic method for
removing prostates. The PA always receives them fresh, inks them, then puts the
whole prostate in alcoholic fixative (currently Zfix from Anatech). The next
day they are grossed in and processed. The prostates we have
Does anyone have experience with the Cell Technology Apo-single stranded DNA
antibody? Please let me know if you do.
Thanks!
Jennifer James BS, HT(ASCP)HTL, QIHC, CRS
Research Assistant
Experimental Pathology Laboratory
Winthrop P.Rockefeller Cancer Institute, Room 429
Universtiy of Arkansas
Hello Histonet.Has anyone ever figured out how much it costs to
produce an HE slide? Are there any articles out there that would help
to figure this out?
I realize it may be different for each institution but a ball park
figure would be greatly appreciated.
Thanks
Amylin Johnson
And your problem will not be solved unless the prostates, after being inked,
are cut in half before placing them in the fixative. I do not see any problem
in cutting in half a properly inked prostate to assure proper fixation and
subsequent processing.
René J.
--- On Wed, 9/2/09, Phyllis
About what to put on tissue to make the marking ink stick to it:
I don't use anything. The trick is to blot the surfaces thoroughly dry
with a paper towel before you try to put the ink on. Of course,
there's nothing you can use that will make ink adhere well to
cauterized surfaces (some breast
Janet Bonner notes:
We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for
small size) www.medline.com.(1-800 - medline) These gloves are not
stiff, they fit the hand 'like a glove' . They say on the box not intended
to be used as a chemical barrier, but they do a
I concur with the Samurai Pathologist. Long ago, we used Bouin's but
have stopped that procedure and prefer to just blot dry but do also use
3% acetic acid, especially if blotting could potentially damage a
delicate tissue. Good luck.
Joe Galbraith
University of Iowa
joseph-galbra...@uiowa.edu
It says on the box that these aloe touch nitrile gloves are Latex-free,
powder-free nitrile examination gloves, single use, non-sterile. Tested for use
with chemotherapy drugs
We've had a terrible time with getting good gloves, and then when we get
some good ones, they are replaced the
Maybe it is the acetic acid in Bouin's that is the active ingredient for dye
adherence!
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Classification: UNCLASSIFIED
Caveats: NONE
We use Distilled White Vinegar. I buy it at the grocery store by the
gallon. It is the acetic acid in bouin's solution that fixes the ink to
the tissue, not the picric acid. I have been using white vinegar for
more years than I care to remember. Hope
I am looking for an alternate disposal method for my Uranyl Nitrate. I was
quoted at about $3000 from our haz. waste disposal company. I would like to
find a cheaper method. I cannot ship it outside the US. Would anyone be able
to take it and add it to their waste? I have about 500ml of a
Does anyone have a good recipe for making up a dissect aid in house?
Sheila Adey HT MLT
_
New! Open Messenger faster on the MSN homepage
http://go.microsoft.com/?linkid=9677405___
I was just doing some research on the original question posted of gloves
suitable for handling xylene. Nitrile is a synthetic rubber made of
acrylnitrile and butadiene, devoid of allergy-causing proteins like in
latex and natural rubber.
The U.S. Dept. of Energy suggests using nitrile when
I don't know what the COST to produce an HE is but we charge outside entities
$8 each to stain them.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amy Johnson
Sent: Wed 9/2/2009
We also use the Aloetouch gloves for all aspects of Histology and have had no
issues with them. They fit like a glove and are very durable but continue to
have good flexability.
Luckily Medline is on contract with HPG (our buying group)!!!
Dawn D. Schneider, HT(ASCP)
Howard Young Medical
Sally:
The simple answer to the question of whether or not biotin blocking is
required is ‘no’. As you stated in your inquiry, blocking need only be
performed when excessive nonspecific staining, attributable to endogenous
biotin,
is observed.
For what its worth, I think that some folks
The cost around $12 to produce a regularly stained section.
The cost to produce a stained section by an very experienced
histotech...priceless.
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Does anyone have any suggestions for antibody, protocol, etc., regarding
staining FFPE sections with pERK? Any information would be greatly appreciated.
Thanks in advance,
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
Over the years I have not found any published cost calculations that have
addressed individual categories that fluctuate constantly in the department and
it seems that there are as many models as there are institutions/companies. In
the past I have found if I separate costs into a minimum of 4
Hi all,
Just to update you, I ordered Abcam's ab13970 chicken anti-GFP and tested it
out in paraffin embedded sections with HIER for 10 minutes in Dako target
retrieval system. I came in with a Dylight 488 secondary from Jackson
Immunoresearch at 1 ug / mL. It worked beautifully at 1:500, 1:1000,
We are in the market for a new embedder. What do you suggest? I tried
searching the archives but only came up with older comments.
Vendors please feel free to contact
frank@sdstate.edumailto:frank@sdstate.edu. We may have to go with a
used instrument so would like comments on good
Histonet,
Has anyone had any experience with the Dragon dictating system? Pro's or Con's?
Thanks for your opinions
Judy McKinney
John Peter Smith Hospital
Fort Worth Texas
817-927-1024
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
We blot the tissue dry, ink specimen and dip into 50% vinegar . Black and
green work best, but all colors work.
Judy McKinney
JPS Hospital
Fort Worth Texas
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
28 matches
Mail list logo
