Tec5 Embedding system from Sakura does it for me - ergonomic and left/right
handed
Annie
2009/9/3 Perry, Margaret margaret.pe...@sdstate.edu
We are in the market for a new embedder. What do you suggest? I tried
searching the archives but only came up with older comments.
Vendors please
Hello,
From: Hobbs, Carl carl.ho...@kcl.ac.uk
Subject: [Histonet] Re:Endogenous biotin blocking
If you are in a research lab and find that you will need to apply biotin
blocking regularly, you can make up your own avidin/biotin solutions if
costs are a major factor.
To overcome the
Judy, my company and I have been experimenting with Dragon since version 4.
The current version is 10.
The bottom line summary is Yes, it will work if you
1. put a lot of time in it, or
2. use a lot of macros/shortcuts/items of that nature.
When it works, it's pretty awesome. If you need
Hello, I am glad to come aboard this network of professionals;
Pathological sciences. I am delighted 'cos i am sure this lists is
providing volume of useful informations to the members. I was
re-directed to the histonet web via my search for info on Formaldehyde
fixation.
Please Barry, I will be
Hi all,
I have been having a problem with counterstaining for nuclei - I'm
looking at mouse lung tissues and staining macrophages with F4/80 (rat
anti-mouse). I use a negative serum control and a rat IgG isotype
control. I see very clear staining of macrophages - but when I
counterstain with
I was put in the position of having to buy a new embedding center last
summer; ours had died and the backup embedder had died also. I had
always before had a single unit with cooling plate and wax chamber in
one and loved them. Will we were in the position to buy fast and I
called all the major
Under separate cover I am sending an article on the subject
René J. (not Barry!)
--- On Thu, 9/3/09, Adeluwoye Oluwatosin princekun...@gmail.com wrote:
From: Adeluwoye Oluwatosin princekun...@gmail.com
Subject: [Histonet] I am glad to come aboard Histonet
To: histonet@lists.utsouthwestern.edu
Our pathology department has been using Dragon for 3 years now and really like
it, both PA's and pathologists alike! It is time saving and easy to adapt to,
even the Pathologists who have their foreign accents!! In fact, our entire
facility is switching to Dragon for dictation. The only
I forgot to include the link:
http://www.histosearch.com/histonet.html
--On Thursday, September 03, 2009 5:15 AM -0700 Adeluwoye Oluwatosin
princekun...@gmail.com wrote:
Hello, I am glad to come aboard this network of professionals;
Pathological sciences. I am delighted 'cos i am sure
That is great, Tosin! You are working on a topic that has been discussed,
debated, and fought over for years. It means there is a LOT of info out
there on it. Start with doing a search for formaldehyde,
paraformaldehyde, and/or formalin in the Histonet Archives. You'll
probably find more than
Dear Histonetters,
We have planned to procure one set of histology systems for our laboratory(We
have the existing one set of instruments all from Leica).
We are considering the purchase of Tissue-Tek® VIP® 6(sakura) for tissue
processing and Robot-Stainer HMS 740(from microm).
Any of our
We blot the surface dry, mark it with ink and dry it again with a hairdryer
for half a minute. -no chemicals
Gudrun Lang
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Amspacher,
September
Judy, We have been using Dragon also for about the same length of time
mentioned by Michael. All pathologists, cytotechs and PA's use it. It is a
fantastic program if used properly and with the right attitude.
Facts:
1) There are some words that it will always have problems with ie.
Mast cells are not conspicuous items in sections of kidneys.
In rats and mice mast cell granules are preserved by ordinary formaldehyde
fixatives and also by non-aqueous liquids such as Carnoy and alcoholic Bouin.
The granules contain heparin, which can be stained by any cationic dye at low
Rebecca, that was great advice. You covered a lot of good detail there.
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
A modern text is
Dawson TP, Neal JW, Llewellyn L (2003) Neuropathology Techniques. Arnold,
London.
The more traditional staining methods are well covered in
Ralis HM, Beesley RA, Ralis ZA (1973) Techniques in Neurohistology.
Butterworths, London.
If you want to use some really tradtitional
I have the same question for the Dolbe system.
Jan
Omaha, NE
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fye Beth
Sent: Thursday, September 03, 2009 12:51 PM
To: histonet@lists.utsouthwestern.edu
Santa Cruz sc-101761.
I tested it on FFPW sections of rat brain sections, after Citric acid HIER and
it is very good ( if the results are specific, of course;-)
It is stated as being Human/Mouse/rat reactive.
I have two images posted here : http://www.immunoportal.com/index.php
for this Ab.
I have decalled whole mouse legs (soft tissue removed). Use 5% formic
acid for 24 hours on a shaker table.
Shaw, Sharon shs...@wpi.edu
Sent by: histonet-boun...@lists.utsouthwestern.edu
09/03/2009 01:09 PM
To
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc
Subject
Hi Histo world,
I have a crazy question, I work in research facility and one of the
investigators needs decal done on mice legs, they never check to see if we had
decal solution before they began the procedure, of course we don't. I tried
different companies to order some but they can't
I would completely agree, to make Dragon work you must want it to work. We use
it successfully with AP Easy in the pathology lab, and successfully with Gmed
in the rest of the office.
Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE 68503
Always Sakura.
René J.
--- On Thu, 9/3/09, abi jag abija...@yahoo.co.in wrote:
From: abi jag abija...@yahoo.co.in
Subject: [Histonet] Your expert advice please_histology systems
To: histonet@lists.utsouthwestern.edu
Date: Thursday, September 3, 2009, 10:48 AM
Dear Histonetters,
We have
Dear Histonet members,
for a few years I have done IHC on fixed-frozen mouse embryos with good
success. A few days ago I moved onto adult tissues (liver and pancreas) and
have met diffulties getting sections of good qualitiy. Here is how I proceeded:
- Pancreas and liver were fixed whole, in
Hi Melissa,
We use Mayer's hematoxylin for 5 min and then rinse in tap water for at least 5
min (to blue the stain).
If your DAB stain is weak, you can counterstain for even shorter time. It works
great!
The other option would be 1% Methil Green in 0.1M Sodium Acetate. My Methil
powder is
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