Re: [Histonet] mice legs

2009-09-16 Thread louise renton
No, I tend to use my own for work!sorry couldn'r resist midweek madness...;-) On Tue, Sep 15, 2009 at 3:42 PM, Shaw, Sharon shs...@wpi.edu wrote: Good Morning Histo World, I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to

[Histonet] Histotechs-Chicago, IL

2009-09-16 Thread Crawford, Jennifer
Good morning Histologists! A hospital that I am recruiting for in the Chicago area has 2 openings for Histotechs on a 1st and 2nd shift schedule, both positions are contract to hire and included medical, dental and vision benefits through Aerotek. The employer is looking for 3+ years of

Re: [Histonet] air quality in the lab

2009-09-16 Thread Merced M Leiker
that's a good one. I'd like to know that one, too. --On Wednesday, September 16, 2009 10:40 AM -0400 godsgal...@aol.com wrote: Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. ___ Histonet

[Histonet] Job In FL

2009-09-16 Thread Alyssa Peterson
Good Morning! Allied Search Partners is currently accepting resumes for our client in Melbourne, FL area. We are prescreening for the following: Position(s): Histotechnologists/Histotechnicians Shift: 1. One tech needed for Day Shift Full Time (8am-4:30pm) 2. One tech needed for Part Time

Re: [Histonet] air quality in the lab

2009-09-16 Thread Rene J Buesa
From the recesses of my sometimes foggy memory, the figure of 4 exchanges/hour has popped up. Perhaps somebody else has some other figure. René J. --- On Wed, 9/16/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: [Histonet] air quality in the

RE: [Histonet] air quality in the lab

2009-09-16 Thread Podawiltz, Thomas
Our last inspection our exchange rate tested out at 52/hour. We had increased from 26/hour because we could still had fumes in the lab. (CMS inspector could smell xylene from our stainer) For the life of me cannot remember what the min. rate is . Tom Tom Podawiltz, HT (ASCP) Histology Section

RE: [Histonet] air quality in the lab

2009-09-16 Thread Weems, Joyce
I seem to remember 12... j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, September 16, 2009 12:18 To: godsgal...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE:

[Histonet] TISSUE FREEZING MEDIUM

2009-09-16 Thread Carol Buckingham
I'm having a great deal of trouble finding a tissue freezing medium that is anything like the original OCT -15-25. Since OCT is no longer available at different temperature uses, I cannot cut a good section in our cryostat(-25)without using freezing spray. Even after trying a few different

[Histonet] need pneumocystis control blocks

2009-09-16 Thread Vickroy, Jim
Does any body know where we can get a few pneumocystis control blocks? Last time I needed a control block for AFB the members really came through. Hopefully someone will have extra pneumocystis blocks also. thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical

[Histonet] Re: ImmPACT DAB kit

2009-09-16 Thread Hobbs, Carl
Many thanks for the replies regarding this reagent. I delayed purchasing this as I was very skeptical: however, I can achieve a 1/5-10 fold dilution factor for my precious low affinity (? thus low dilution factor) Abs. I will not be using ImmPACT chromogen kit routinely as, for the vast

RE: [Histonet] Polarizing filters

2009-09-16 Thread Monfils, Paul
The polarizer and analyzer are identical filters, and either of them can be used in either location. One must be between the light source and the slide being viewed. The other must be between the slide being viewed and your eye or camera. I place one filter directly on top of my illuminator.

[Histonet] Re: Air Quality

2009-09-16 Thread Cindy DuBois
Does anyone know where we can find written regulations regarding air quality in the lab? We start @ 330 am, but the HVAC system doesn't come on until 7. So we are working with just portable fans (or heaters in the winter) those first few hours. Cindy

[Histonet] mib-1

2009-09-16 Thread godsgalnow
I have to turn to the experts here.? I am having problems with the MIB-1.? I am having an issue with the patient samples working.? We use an alcohol based fixativeit has always worked until recently. I have tried everything I can think of to no avail.? I have decreased the titer,

[Histonet] Air changes per hour

2009-09-16 Thread Lisa S. Black
OSHA's Occupational Exposure to Hazardous Chemicals in Laboratories; Final Rule (Federal Register January 31, 1990) went into effect May 1, 1990. Section C.4 (f) of Non-Mandatory Appendix A indicates that 4-12 room air changes per hours (ACH) of fresh air are normally adequate if local exhaust

Re: [Histonet] Re: Air Quality

2009-09-16 Thread Rene J Buesa
Regardless of the regulations, fans will not providce them so the real solution is to have HVAC system running when you start, or do they want to subject you to unsanitary conditions? Have the ever heard of an OSHA intervention or about their fines? René J.? --- On Wed, 9/16/09, Cindy DuBois

[Histonet] hyaluronidase for IHC pretreatment

2009-09-16 Thread Amy Lee
Hello,   I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution. Could anybody help me please?   Best regards,   Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] hyaluronidase for IHC pretreatment

2009-09-16 Thread Geoff McAuliffe
You buy hyaluronidase (freeze-dried) from Sigma or some other vendor and make it up in the appropriate buffer. If my memory serves me, and there have been no major advances in the field, hyaluronidase comes in two flavors, one from bovine testis and one from Streptomyces sp. These are NOT the

RE: [Histonet] Peloris tissue processor

2009-09-16 Thread Stella Argyriou
Dr. Evette Demaisip, Thank you for your posting. We have forwarded this to your Leica representative for the Philippines. They should be in contact with you shortly. Alternatively in future you may also contact histosupp...@leica-microsystems.com for further assistance. Best Regards, Stella