[Histonet] RE: PIN-4

I have also used this system and worked it up on the N Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: PIN-4

Sorry email cut off. I worked it up on the Dako Autostainer Link 48. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Autopsy baskets wanted

Good morning, We are looking to purchase some little metal/stainless steel baskets with expandable lids used for processing larger specimens. We just found out EMS does not sell them anymore. Does anyone here in histo-land know where we can find these? Thanks in advance, John

[Histonet] RELIA Histology Careers Bulletin Right Place Right Time Right Opportunity

Hi Histonetters!! Right Place, Right Time, Right Opportunity! Sometimes your next career move is just a matter of being in the right place at the right time and ready for the next opportunity. Odds are that if you are contemplating a job change for whatever reason – better compensation, a

AW: [Histonet] ISH on decaled bone

I believe there is no chance for retrieval. DNA or RNA is degraded by hydrochloric acid. You would need some glue to stick the fragments ;). Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von

[Histonet] Orienting GI biopsies during embedding

Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology

Re: [Histonet] Orienting GI biopsies during embedding

There is a very fine Technical Note by I. Dimenstein in the last issue of the JOH that can help you solve this problem. René J. --- On Thu, 9/24/09, ssylv...@cinci.rr.com ssylv...@cinci.rr.com wrote: From: ssylv...@cinci.rr.com ssylv...@cinci.rr.com Subject: [Histonet] Orienting GI biopsies

[Histonet] Re: Histobath Frozen Sections

I've posted a number of times about the Histobath. I don't know anything new besides the stuff I've already posted. Melanie S. White, MT(ASCP) asks: P.S. Are there any other Samurai Pathologists available? We need one. Hey, find me a way to get a South Carolina medical license at the age of 70

[Histonet] Re: Orienting GI biopsies during embedding

Sabina Sylvest at Cincinnati Children's Hospital asks: We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate

[Histonet] SOX10

Hi, Would anyone using SOX10 antibody please tell me where they buy it? If you have a procedure that you would be willing to share as well I would be very appreciative. Thank you! Erin Martin UCSF Dermatopathology ___ Histonet mailing list

[Histonet] Steiner and Steiner

Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in

RE: [Histonet] Steiner and Steiner

Check with Newcomer Supply as they have a Modified Steiner Kit without Uranyl Nitrate. Jodie Robertson, HT(ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor Chico, CA 95926 -Original Message- From:

[Histonet] Need a NSH S/C roommate

I would like to share a room for the NSH Symposium/Convention in Birmingham to help save on costs. I have a room reserved at DoubleTree from Friday - Wednesday, but if you already have a room reserved and are looking for someone to share costs, I can do that too. Send me an email at

RE: [Histonet] MSI testing

Not us!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, September 24, 2009 13:38 To: Histonet Subject: [Histonet] MSI testing How many of you that do IHC and/or PCR testing

[Histonet] CD31 Mouse Liver Staining

Hello, I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections. I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal. My question is regarding

[Histonet] Guniea pig anti-Doublecortin Goat-Guinea pig secondary antibody on rat tissue

it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on blood vessel-like structures. Anyone has such experiences before? Do goat-anti-guinea pig IgG will cross react with rat IgG ? Or

[Histonet] MOLECULAR LAB QUESTION

Two questions (if anyone can answer): (1) What tests are you doing if you in fact have a 'molecular lab'? (2) Who runs the tests...Histotech or CLS ? Thanks! Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689

[Histonet] cap survays

To all, I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis. Our paths want us to score then and they not be involved. Is this what everone else is doing? Edie DISCLAIMER: This message is intended for

[Histonet] RE: cap survays

Technologists can certainly be trained to do the scoring properly but our pathologists score them and are fully involved in the evaluations, as well as reviewing challenge slides for submission. There are also sometimes interpretation questions that have to be done on-line that the pathologists

[Histonet] Re: ISH on decaled bone

Gudrun is absolutely correct. The HCl destroyed your nucleic acids. The best decalcifier for RNA or DNA is EDTA. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110

[Histonet] Re: License

Joyce, You might have some luck calling the following places: Physician's Reference Lab, Overland Park, KS - Donna Miller Shawnee Mission Medical Center, Overland Park, KS North Kansas City Hospital - N. KC, MO - Nancy Warren University of Kansas Medical Center, Kansas City, KS - Timothy

[Histonet] B Plus fixative on bone marrow cores

I would appreciate comments from anyone using this fixative for bone marrow cores. Specifically I am interested in learning how long you are fixing your samples in B Plus. Manufacturer recommends at least two hours but we are seeing artifacts that concern me that 2 hrs may be insufficient.

RE: [Histonet] Orienting GI biopsies during embedding

I would recommend you try to embed them on edge. Even if very small, you will be surprised how many are correctly embedded Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at

RE: [Histonet] Steiner and Steiner

Nikki, The sensitisation step, from my experience, improves the silver deposition on the bacteria and decreases background staining. Margeson Chappman (1996 Use of Zinc Formalin as a Sensitizer in Silver Stains for Spirochetes J Histotechnol 19(2):135-138) substituted zinc formalin for uranyl

RE: [Histonet] cap survays

Edie, What a challenge. I would strongly recommend you give it a go. I like to score fixation, processing and staining quality of slides I and my staff produce. It allows us to have control over the science of histotechnology not the pathologists (they have enough to worry about - the diagnosis,

[Histonet] See you at the NSH

Hi all, Just a note to say that I will be leaving next week for Birmingham Alabama for the NSH conference. It has taken me 30 years to get to a NSH and I am really looking forward to it. I hope to see you all (I will be the old, short, bald guy with the Aussie accent) Regards Tony Henwood JP,

[Histonet] Rhodanine Copper stain

Hello! I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of tissue core biopsies are cut at 3 microns and if possible we would not like to cut

[Histonet] Slide and Block Disposal

Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or

[Histonet] ASCP CMP continuing education credits

I am attempting to renew my HT ASCP certification for the first time and I was wondering if taking the Anderson Continuing Education course (Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications-they list it as providing 36 hours) would fulfill the requirement completely. I