[Histonet] IHC on plasma cells

Hi everyone, I have been asked to do IHC on plasma cells of FFPE human tissue.  Anyone have a good antibody (and method) for this procedure? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list

[Histonet] RE: Histonet Digest, Vol 70, Issue 35

I have used the ThermoFisher Excelsior at two different facilities. It is a wonderful processor and maintenance is a breeze, and uses less alcohol and xylene. Please take time to look at it. Elaine Monroe, Lead Histotech Spring Memorial Hospital From:

RE: [Histonet] ASCP CMP continuing education credits

ASCP accept AndersonCE From: histonet-boun...@lists.utsouthwestern.edu on behalf of Lee Peggy Wenk Sent: Fri 9/25/2009 5:01 AM To: kmcn...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP CMP continuing education credits According to

[Histonet] Re: IHC on plasma cells

Kim, CD138 is a good marker for plasma cells. Contact Chris van der Loos for a protocol. He mentioned this at NSH in Denver 2007. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110

[Histonet] ALK 1 Controls

Does anyone know where I can purchase ALK 1 controls? I cannot get them from American Master Tech. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] COX staining in frosen muscles

Dear Colleagues. Please help. I have been asking to perform staining for  COX (which is cytochrome oxidase I believe) activity on mouse fresh frozen leg muscles. I am not very  familiar with enzyme histochemistry. I found one method (Seligman,1968) in J.Bancroft , 5 edition book, and the

[Histonet] OSCAR control

Any ideas on what I can use to optimize OSACR antibody? Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 Notice from St. Joseph Health System: Please note that the information contained in

Re: [Histonet] OSCAR control

Dr. Allen Gown, from PhenoPath laboratories developed this antibody.  Perhaps you might contact Patti Loykasek at PhenoPath at (206) 374-9000 and she might help you. Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact:

[Histonet] Re: Responses to micron thickness for Rhodanine copper

Hi and thank you all for your responses! Everyone agreed that the thicker sections allow the copper pigments to be better visualized. However the responders were split 50/50 in the thickness. There were labs that did cut the sections at 3 microns with continuous good results and the others at

RE: [Histonet] COX staining in frosen muscles

No Problem Delores Send me your fax number and I will send over our protocol. Florence LeomitiHT (ASCP) OHSU Neuromuscular Lab Tech. Phone 503-494-6781 Fax 503-494-6787 Pager 16822 -Original Message- From: Diaz, Dolores [mailto:dolores.d...@ttuhsc.edu] Sent: Monday, September 28,

Re:[Histonet] Guniea pig anti-Doublecortin Goat-Guinea pig secondaryantibody on rat tissue

Subject: [Histonet] Guniea pig anti-Doublecortin Goat-Guinea pig secondaryantibody on rat tissue it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on blood vessel-like structures.

RE: [Histonet] Re: Responses to micron thickness for Rhodanine copper

Once upon a time there was an investigator who used sections of testis of unspecified thickness to stain with iron hemtoxylin. From his observations of the results, he concluded that there were two distinct populations of Sertoli cells based on his observation of two distinct populations of

[Histonet] Oscar

Hi All. I understand that there was a recent question regarding the antibody Oscar (pan cytokeratin). I've been off histonet while I was on vacation. We routinely work up Oscar using normal liver. There should be staining of the bile ducts and the hepatocytes. I'd be happy to offer more info if

RE: [Histonet] Oscar

Patti, Thank you for taking time to talk to me. I appreciate the help! Kind regards, Maria Katleba HT(ASCP) MS Queen of the Valley Medical Center Napa CA 94558 707-257-4076 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Oscar

Oscar? when I was the supervisor of the immuno lab at the AFIP, our rabbit that produced our anti-keratin died. The civilian researcher and I were working up multiple cytokeratin cocktails to replace Fluffy. We wanted to call it the BOZO antibody, but our medical director refused. I can see it

[Histonet] More Copper stain thickness

Hi Histonet! Regarding the discussion about the thickness of FFPE sections for Rhodanine copper, ie 3 um vs. 6 um, someone asked me: If everyone agreed that thicker sections are better, how it can be a 50/50 split about the thickness? Isn't that an unsustainable contradiction? (and I thank