Of course you can always do IHC on your rat that died, you just have to note
that that rat died and was not profuse fixed so that the review of the
results will take that in consideration, hopefully you have another rat that
besides dyeing and not getting profuse fixed had all other conditions the
Gudrun
I work in a dermatopathology lab, and we don't see any issues leaving our
specimens in 10% NBF for days at a time. We don't work on the weekends, so
any specimens that are grossed remain on our processors in NBF from Friday
at 8:00 PM until about 5:30 PM on Sunday. I also agree with Kris t
Dear Salim,
As you have been informed, doing immunohistochemistry is possible on
this tissue. After all it's possible to do IHC on any tissue whether
the conditions you want to test under are ideal or not.
Being chastised on this list and calling your work "bad science" is
totally out of line
I am afraid that any IHC that you will attempt to do will not serve for your
original experimental purposes of having a perfused brain. I am afraid you will
have to start all over again.
At least now you know a probable survival time, so start the new experiment in
a way that the rat is "suppose
John,
OUCH!
> From: jkier...@uwo.ca
> To: syi...@ucalgary.ca
> Date: Sat, 28 Nov 2009 01:33:55 -0500
> Subject: Re: [Histonet] a basic question about immunohistochemistry
> CC: histonet@lists.utsouthwestern.edu
>
> Dear Dr Inan,
>
> Anyone can fix, process and do immunohistochemistry on parts
