I guess I did not realize what ongoing discussion my comment about the
CryoJane tape transfer would spark! Thanks to the replies I have received
in private and in public, I see that it indeed helps one make lovely bone
sections.
In the past 9 years that I have cryosectioned, I have done a
Histonet,
One of our pathologists is looking at a method for cutting GI biopsies that
differs in complexity from the way we currently do them. Currently we cut
just 2 levels at 4 microns thick, 40 microns apart and only showing 2
sections on each level. In the new plan, if there were a maximum
Dear Histonet Subscribers,
I am a one of the founders of a Healthcare Recruiting firm that specializes in
placing Lab Professionals. We work exclusively on permanent positions and have
clients across the country. We are completely free of charge to candidates and
are currently working
It takes a while to get experienced, but it doubles, sometimes triples
the time.
Joyce
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat
Laurie
Sent: Wednesday, December 09, 2009 13:34
To:
On the subject of protocols being passed down from person to person, lab
to lab, etc., a freshly minted PhD came to me once looking for some NaH
with which to make buffer. I explained that there was no such chemical
as NaH but she insisted: here is the protocol. A simple typographical
error
Fix in buffered glutaraldehyde for 2 hours, overnight is acceptable.
After multiple rinses, store in buffer in the refrigerator. Change the
buffer once a week, there are things that will grow in cacodylate
buffer. You may complete osmication if you want, then rinse and store in
buffer. I would
Hi all,
We have suddenly started losing tissue sections from our Superfrost Plus
Slides.
Our students have been cutting fixed, frozen, cryosections (20um) and
thaw-mounting these onto Superfrost Plus slides. We have suddenly
started losing lots of sections from these slides (again!!). The
Angelina,
I have seen this post a few times before and sometimes the problem was the
drying technique or making sure your water bath is clean and free of any
debris. I also remember hearing about someone pre-treating their slides with
a Trilogy (EDTA) buffer in a pressure cooker.
Good Luck!
An experienced histotach can cut regular blocks at a rate of 24/hour. What you
describe will reduce the productivity to 8 to 10 blocks/hour.
René J.
--- On Wed, 12/9/09, Weems, Joyce jwe...@sjha.org wrote:
From: Weems, Joyce jwe...@sjha.org
Subject: RE: [Histonet] Cutting GI biopsies with
Prepare a 1% aq. sol. of Elmer's Glue and coat the slides with it. Oven dry
and use the slides as usual.
René J.
--- On Wed, 12/9/09, Angelina Fong f...@zoology.ubc.ca wrote:
From: Angelina Fong f...@zoology.ubc.ca
Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides
To:
I would suggest you check the lot#. You may have received a bad lot and need to
get them replaced by the vendor.
Hi all,
We have suddenly started losing tissue sections from our Superfrost Plus
Slides.
Our students have been cutting fixed, frozen, cryosections (20um) and
Hi Histonet,
I know we have discussed the average blocks histotechs cut per hour, but
does anyone know if there is a maximum blocks that can be cut per hour? Or
per day? We have regulations for cytotechs who are reading slides for
maximum slides they can read per hour and are wondering if there is
You wrote:
We have suddenly started losing tissue sections from our Superfrost Plus
Slides.
Our students have been cutting fixed, frozen, cryosections (20um) and
thaw-mounting these onto Superfrost Plus slides. We have suddenly
started losing lots of sections from these slides (again!!).
Does anyone know if Arizona requires a state license for
histotechnicians/technologists?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
No we dont. At least not yet. However certification is strongly preferred
around here.
-Original Message-
Date: Wednesday, December 09, 2009 4:39:04 pm
To: 'histonet' Histonet@lists.utsouthwestern.edu
From: dcoj...@tampabay.rr.com
Subject: [Histonet] arizona state license
Does anyone
Genitourinary / Urogenital Pathology Opportunity
(Relocation Assistance Available)
Location: Dallas Texas
Fulltime / Permanent Position
Generous relocation assistance available!
Our client is one of healthcare’s leading clinical diagnostics organizations.
Grow your career, as they
There are no industry or regulatory standards that dictate a production
maximum for blocks cut or slides produced hourly or daily. It is impossible to
compare labs and set productivity standards becauseof the lack of standardized
processes and protocols in Histology. I suggest that techs
Hi Angelina,
We were having the same problem with frozen mouse aortas. I was cutting at 5ums
onto superfrost slides and was losing about half of my sections. I ended up
swapping to superfrost plus ultra, plus I bake the slides in the histology oven
at 60 degress celcius for 10 minutes and
Several of you have recently written wanting the instructions for the
Leitz 1512. I had temporarily misplaced these because my lab moved for the
second time in the past year. I have now located these. Please re-connect
with me so I can fax or mail you these instructions.
Thank you.
19 matches
Mail list logo
