Sorry for the second email, I didn't address all your questions ...
The antibody I use from Serotec is MCA497, works very well and many people on
Histonet have claimed the same over the years.
I am not familiar with the secondary you use from Serotec. It says it's goat
anti-rat IgG, mouse ads
Dear Mauricio,
The two antibodies you chose were NOT cross adsorbed against mouse. This is
critical for successful staining of mouse tissue with rat antibodies. These are
the two alternate catalog #s of the ones I would recommend if FITC is your
thing:
712-095-153 (whole IgG FITC conjugated)
Hi everyone
These questions are primarily for those people who currently work in, or
have experience in private practices (derm, GI) that have in-house path
labs.
For IHC, how cost-effective is it to do immunos in-house *using kits*? No
immunostainer would be available. I imagine it's substantial
At DCH we have a full time HistoTech position. Flexible day shift hours Monday
through Friday. Must be ASCP registered HT or HTL (or eligible...must become
registered within 18 months of hire date).
Lab Automation includes IHC stains (Ventana), staining and coverslipping
(TissueTek Prisma). We
We use the Tissue-Tek Glas with great results. Knowing how Sakura loves to
just relabel Leica equipment, it might be the same as the CV5030, but I'm
not sure. Either way, I've been very impressed with the Tissue-Tek machine.
Drew
On Thu, Mar 4, 2010 at 15:00, Derek Papalegis wrote:
> Hi Everyo
Very sorry to hear about this incident and thank goodness the tech didn't get
injured AND used quick-thinking to grab the extinguisher and put out the fire.
This incident exemplifies the (hidden) dangers inherent in the operation of
labs that, often are overlooked ("we haven't had a fire in 20
We currently have a Leica and a Jung. They are serviced twice a year. Prior to
these, we had two Tissue Teks that were donated to us. My best guess is that
they were about 20 years old when they were replaced.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
Try the Leica CV5030.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Derek
Papalegis
Sent: Thursday, March 04, 2010 3:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] glass coverslipper
Hi E
We are building a new hospital also. Currently the old hospital grossing is
done there and the cassettes are shipped over to our lab site. We use AP
assistants (high school diploma) to accession, assist the path and cut & stain
the frozen sections. (Actually our AP assistants were formally Phleb
Meditech is an awful program for Pathology. I loved using Pathologix (the
Software company... not the Reference lab that is stealing histology jobs from
hospitals). Even Co-path is acceptable.
Meditech does not lend itself to Pathology as it does to clinical lab. It is
cumbersome and not user
Hi Everyone,
I am looking for a glass coverslipper and was wondering what people
recommend. I am in a small research lab so having a small footprint is
very important. It doesn't have to be very fast but I need something
dependable that will give consistent results.
Thanks,
Derek
--
Derek Pa
Hi All,
Are there any cryostat makes that seem to be more reliable than others? We have
not been having much luck with our particular Microms after moving them
cross-country, getting recalled, catching on fire, corroding, etc. While one
machine serves as an excellent calendar stand (with padde
Thanks to all of you for your suggestions. We will be putting the Biocare
Universal Negative Control Serum through a trial.
Once again, Histonet proves it's not only invaluable, and populated by helpful,
knowledge and experienced histotechs, it's also extremely expedient!
Thanks,
Eric
Hello to everyone in histoland. We are currently building a new medical
tower next to the hospital. Our histology lab will be moving there, but
the existing frozen section area will remain in the main hospital. How
are those with multiple sites dealing with staffing and specimen and
slide flow.
We have Meditech but have never used order entry. Our IS determined with the
mistakes the floors make in entering they would not include us in order entry.
Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
From:
I am using Fix-All from Surgipath for bone marrow fixation. I empty the
syringe into the fixative as soon as it is handed to me (no messing around with
clots). I fix for 1-2 hours in Fix-All then transfer to a screen cassette and
our normal processing with 10%NBF. The pathologist is very hap
Dear histonetters,
I was wondering if it is possible to do an EBER ISH on a cell-smear. What
pretreatment should I use?
1: Fix the cells in formalin (let's say overnight, or else one or two hours),
and than use the proteinase K digestion step.
2: Or maybe it is enough to fix the cells in cold
Yes, Linda and I select the harshest protocol in the list of antibodies
we're running per case-we figure that the harshest will produce the
biggest problems with background, etc.
Sally Ann Drew, MT(ASCP)
IHC/ISH Clinical & Research Laboratory
UWHC
600 Highland Ave. DB1-223, Mail Code 3224
Madison,
We too use the Universal negative from Biocare, and it has passed
through 2 CAP inspections so no problem on that front.
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern
Hi Anita,
We are currently examining the negative control issue in our laboratory. We
also have Ventanas. We currently use a normal goat serum as our "non-immune"
serum. We are considering changing our SOP's to use the Ventana Neg Control
Rabbit and Neg Control Mouse in dispensers.
Linda
Stephen,
we doubled the routine protocol for braintissue from 13 to 24 hour. We
stayed at one hour for the formalin steps, because the tissue is fixed for
several days before processing. This protocol is also used for prostate big
cassettes.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-b
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