Re: [Histonet] IF staining on peritoneal macrophages

Sorry for the second email, I didn't address all your questions ...   The antibody I use from Serotec is MCA497, works very well and many people on Histonet have claimed the same over the years.   I am not familiar with the secondary you use from Serotec. It says it's goat anti-rat IgG, mouse ads

Re: [Histonet] IF staining on peritoneal macrophages

Dear Mauricio,   The two antibodies you chose were NOT cross adsorbed against mouse. This is critical for successful staining of mouse tissue with rat antibodies. These are the two alternate catalog #s of the ones I would recommend if FITC is your thing:   712-095-153 (whole IgG FITC conjugated)

[Histonet] IHC cost-effectiveness

Hi everyone These questions are primarily for those people who currently work in, or have experience in private practices (derm, GI) that have in-house path labs. For IHC, how cost-effective is it to do immunos in-house *using kits*? No immunostainer would be available. I imagine it's substantial

[Histonet] Full Time Day Shift DCH Regional Medical Center Tuscaloosa, Alabama

At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We

Re: [Histonet] glass coverslipper

We use the Tissue-Tek Glas with great results. Knowing how Sakura loves to just relabel Leica equipment, it might be the same as the CV5030, but I'm not sure. Either way, I've been very impressed with the Tissue-Tek machine. Drew On Thu, Mar 4, 2010 at 15:00, Derek Papalegis wrote: > Hi Everyo

[Histonet] Fire in the lab

Very sorry to hear about this incident and thank goodness the tech didn't get injured AND used quick-thinking to grab the extinguisher and put out the fire.  This incident exemplifies the (hidden) dangers inherent in the operation of labs that, often are overlooked ("we haven't had a fire in 20

RE: [Histonet] Cryostat better than others?

We currently have a Leica and a Jung. They are serviced twice a year. Prior to these, we had two Tissue Teks that were donated to us. My best guess is that they were about 20 years old when they were replaced. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:his

RE: [Histonet] glass coverslipper

Try the Leica CV5030. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Derek Papalegis Sent: Thursday, March 04, 2010 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glass coverslipper Hi E

[Histonet] RE: Dual Locations

We are building a new hospital also. Currently the old hospital grossing is done there and the cassettes are shipped over to our lab site. We use AP assistants (high school diploma) to accession, assist the path and cut & stain the frozen sections. (Actually our AP assistants were formally Phleb

[Histonet] RE: Meditech and Computer Requisitions vs Paper Req's

Meditech is an awful program for Pathology. I loved using Pathologix (the Software company... not the Reference lab that is stealing histology jobs from hospitals). Even Co-path is acceptable. Meditech does not lend itself to Pathology as it does to clinical lab. It is cumbersome and not user

[Histonet] glass coverslipper

Hi Everyone, I am looking for a glass coverslipper and was wondering what people recommend. I am in a small research lab so having a small footprint is very important. It doesn't have to be very fast but I need something dependable that will give consistent results. Thanks, Derek -- Derek Pa

[Histonet] Cryostat better than others?

Hi All, Are there any cryostat makes that seem to be more reliable than others? We have not been having much luck with our particular Microms after moving them cross-country, getting recalled, catching on fire, corroding, etc. While one machine serves as an excellent calendar stand (with padde

[Histonet] Negative Controls...thanks

Thanks to all of you for your suggestions. We will be putting the Biocare Universal Negative Control Serum through a trial. Once again, Histonet proves it's not only invaluable, and populated by helpful, knowledge and experienced histotechs, it's also extremely expedient! Thanks, Eric

[Histonet] Dual Locations

Hello to everyone in histoland. We are currently building a new medical tower next to the hospital. Our histology lab will be moving there, but the existing frozen section area will remain in the main hospital. How are those with multiple sites dealing with staffing and specimen and slide flow.

[Histonet] RE: Meditech and Computer Requisitions vs Paper Req's

We have Meditech but have never used order entry. Our IS determined with the mistakes the floors make in entering they would not include us in order entry. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 From:

[Histonet] Bone Marrow Fixative

I am using Fix-All from Surgipath for bone marrow fixation. I empty the syringe into the fixative as soon as it is handed to me (no messing around with clots). I fix for 1-2 hours in Fix-All then transfer to a screen cassette and our normal processing with 10%NBF. The pathologist is very hap

[Histonet] How to do an EBER on cytological material?

Dear histonetters, I was wondering if it is possible to do an EBER ISH on a cell-smear. What pretreatment should I use? 1: Fix the cells in formalin (let's say overnight, or else one or two hours), and than use the proteinase K digestion step. 2: Or maybe it is enough to fix the cells in cold

RE: [Histonet] Negative controls

Yes, Linda and I select the harshest protocol in the list of antibodies we're running per case-we figure that the harshest will produce the biggest problems with background, etc. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison,

RE: [Histonet] Negative controls

We too use the Universal negative from Biocare, and it has passed through 2 CAP inspections so no problem on that front. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern

[Histonet] Negative controls

Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our "non-immune" serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda

AW: [Histonet] Re: paraffin processing times for brain

Stephen, we doubled the routine protocol for braintissue from 13 to 24 hour. We stayed at one hour for the formalin steps, because the tissue is fixed for several days before processing. This protocol is also used for prostate big cassettes. Gudrun -Ursprüngliche Nachricht- Von: histonet-b

[Histonet] Histo Supervisor Needed In Florida

Allied Search Partners is currently accepting resumes for a Histology Supervisor in Polk County (Auburndale, FL area). Position Title: Histology Supervisor Duties include but are not limited to: - Provide comprehensive management of the Histology section to ensure delivery of quality