Regarding the need to avoid accessioning similar samples consecutively,
I addressed this last week with my bosses. Specifically, I asked that
spleen specimens not be accessioned consecutively (because of shattering
and static and the possibility of fragments being carried over); my
response was
Sara,
Unfortunately the response you got is more common than I would like to
see.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
We have agar based markers - red, blue, green, yellow, orange, black,
that we include in the block for similar tissues - as we have several
that be separated. It is noted in the gross and confirmed at micro that
the markers are present. After we've gone through the colors once, we
put two, three,
Hi Histonetters
I am having a senior moment. Can anyone tell me what McCoy fixative is made
of and what it is used for? Thanks in advance for the help.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
Carol,
I take these kinds of CAP issues to mean, develop your own program for
proficiency testing and show how you have validated it, then show it to CAP,
they usually want to know that you have a plan in place even if it is
something you developed yourself.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
Several people have expressed frustration with the unsubscribe process. The
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Hi Sheila,
HPS (Hematoxylin-Phloxine-Saffron) is the routine stain we use. There are a
few institutions in Canada that also still use this stain; around Ottawa and a
few other places in Ontario and Quebec. Perhaps one of these institutions sent
slides to your pathologist for
I think there is confusion regarding the original question posed. They
were not asking about the HPS stain. Some hospitals use an
eosin/saffron stain in their routine HE. I had another lab recently
asking about the formula to prepare this solution instead of eosin. Any
advise?
Diana
We have the Meditech Computer system and we have built a data section between
Micro and Diagnosis namde Synoptic Tumor Protocol nd have canned texts
available for all tumor types that pull in the required information needed.
All the Pathologist's have to do is enter the information and hit
We are making our own research / immunohistochemistry controls and have found
that the tips, as well as the molds are becoming quite expensive. The tips (
especially in the hands of a resident) have a tendency to be rather soft and
loose their shape rather quickly. Any other users having this
Dako's rabbit anti-CD3 cross reacts with mouse
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
Hi Li,
Try 1-2 ml/min perfusion rate. Yes 10ml/min is way too fast. I know it's
tricky; I've done it by hand and by pump.
Perfuse with PBS or Saline (same thing) first, then 4% PFA til mouse is
stiff, then after taking the brain out immerse it in increasing grades of
sucrose
I apologize in advance if I offend anyone with this inevitably touchy
question.
In the last two years we have lost one older histotechnologist, and
the routine reliable services of another of that group and are now
facing the issue of what can be expected from their replacements. I
have little
Hi everyone,
I need some advice.
I have been doing tissue processing (on an old Shandon Hypercenter),
embedding and sectioning for 15 yrs. and never really had any problems.
I recently processed mouse tissues on a brand new tissue processor
(Leica ASP300S). When I went to cut 5 micron
Sheri
Your processing cycle is too long. We process 20 minutes per station for mouse
tissue only 2 absolutes and 2 xylenes and 3 paraffins. Even 3 absolutes and 3
xylenes at 20 minutes a station we find over processes the tissue.
Liz
From:
Hello,
I have just started using a vibratome (Leica VT1000S) to cut sections of
fish embryos after whole mount in situ hybridization, but I've
encountered a problem with this. I have been setting the vibratome to
cut sections of 50um to 70um in thickness (on the recommendation of a
colleague
I routine use anti-human antibodies to stain mouse tissue, so it can be
done. It completely depends on the antigen they used to immunize. If the
human and pig proteins are highly homologous or identical, then it has a
good chance of working. If they're not, you're in uncharted territories. If
you
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