Dear Histonetters,
We use an Eosin Y/Erythrosin B mixture containing calcium chloride in an
aqueous solution, and this, if differentiated correctly in water, gives 3
definite shades of pink. Our students are asked to find an artery to check
their differentiation and they should be able to see musc
Hi everyone,
I've been performing Gomori's reticulin impregnation and never got the results
I see in textbooks. The reticulin fibers stain fine, but I get a dark
purple/gray background instead of a light pink background. Does anyone know why
?
Here is the technique I use :
1) De
In a good H&E, the eosin/phloxine is supposed to be 3 shades:
- RBC's/eosinophils/Paneth cells the deepest shade of pink
- muscle medium shade of pink
- collagen/connective tissue lightest shade of pink
Unfortunately, if the eosin and/or phloxine and/or eosin/phloxine does not
have the correct co
