Hi Histonetters!
I hope you are doing well. I wanted to send a special bulletin wishing
you a Happy National Medical Laboratory Professionals Week. I hope your
lab has been having some fun, informative and special events to
celebrate because you deserve it.I have heard of some really cool
Hi All,
Does anyone have an IHC protocol for Fluoro Jade B (or C)? I have
read it can be adapted from FITC to IHC but I can't find a protocol.
Thanks,
Jennifer Campbell
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Use this link to go to the CAP website to download the 2010 ER/PgR guidelines
article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page:
http://tinyurl.com/34kxhtn
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-Original Message-
From:
Hello,
I have a question that what is best way to block endougenous biotin and HRP in
liver or kidney tissue. The method I am using has to use avidin-biotin and HRP
reactions. This is fine on heart tissue. I used biotin blocking kit from DAKO
or Vector. Also I used 1% H2O2. But liver tissue
Sharon,
I used to freeze rabbit hearts (a LITTLE bigger than rat) this way.
Pour isopentane into a metal beaker inside a styrofoam container filled
with liquid nitrogen to part way up the sides of the beaker. When the
isopentane starts to freeze on the bottom of the beaker it is ready.
Place
I was an MLT who transitioned into histology, did the program at IUPUI and
passed the HT exam on the first try (Thankfully). I took the exam as soon as
could following the program, studied Carson, exams from the program and
purchased the online tests through ASCP. My coworker started in the
Hi All,
Does anyone out there purchase their reagent alcohol in 50-55 gal
drums? How much does this cost you, including the shipping handling
and any other costs? We are trying to determine whether this would be a
good idea or not.
Thank you,
Jennifer Campbell
Just be sure you get dye certified by the Biological Stain Commission.
The bottle must have a Certification label or it is not certified to
perform as expected.
Sigma is reliable.
Geoff
zodia...@comcast.net wrote:
Hello to all,
I was wondering if anyone knows where to purchase toluidine
This question if for anyone doing C4d immunoflourescence staining. Could
someone share what protein concentration the Cy2 labeled anti-mouse IgG
is prepared at and what buffer is used for that? Our final dilution of
the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original
protein
Eric:
Are you using Cy2 labeled goat anti-mouse IgG (HL) from Jackson
ImmunoResearch Labs?
According to their catalog (product number 115-225-003), the stock is 2.0
mg/ml. At 1:100, your working concentration would be 20 ug/ml.
Jim B
Breisch, Eric ebrei...@rchsd.org
Sent by:
Brett-
Most wrinkles in decalcified bone sections come from stretching of the
decalified bone that occurs during the sectioning process. I would suggest a
rather simple solution. Allowing the sections to flatten on the waterbath
might take longer or a higher temperature. If paraffin
Thank you to everyone who responded to my question regarding where to purchase
Toluidine blue. It is very much appreciated.
Jenny
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I need a strong IHC tech with a NY license in Long Island. Great pay.
Sent from my Verizon Wireless BlackBerry
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