[Histonet] Thank you all sho gave me advice on reducing background of liver tissue!

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[Histonet] Gram stain

Now that I'm done with my rant I have a real question. We are trying to do a gram stain on fish and the safranine O is staining everything red. What other stain would you use? I usually have time to look at the books but unfortunately it's almost quiting time and the slides need to be done by

[Histonet] RE: new DAB

Bless you... Call you tech support and they will come do it for you! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Friday, April 23, 2010 17:16 To: histonet@lists.utsouthwestern.ed

[Histonet] new DAB

When did DAKO retire their DAB and only stock the DAB plus? Since it's Friday I can rant. I am so disgusted that no one was notified of the change. With QC I'm going to have to revalidate or prove that the New DAB works as well as or better than the old. Of course I had to be a klutz and spi

[Histonet] RE: ER clone 1D5 or SP1 ?

We have used 6F11 in the past and have switched to SP1. In running parallel tests, we noticed about a 97% concordance between the two. Good luck. Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 ___

[Histonet] accessioning of specimens

We seem to be having trouble with the correct printed cassettes being placed on the correct containers. Or wrong numbers printed on cassettes. How does everyone accession their cases? Guidelines for checking cassettes & containers? Any suggestions other than looking over their shoulder with each

[Histonet] Contract Histo Tech Needed in Albuquerque, NM

My name is Eric Weber and I am a recruiter for Maxim Government Services. We provide temporary contracted lab personnel to the federal medical facilities. I'm current recruiting for a contract Histology Technologist for an immediate start through August 13th, 2010. Details: * Special

[Histonet] Two antibodies from the same host with tyramide

Hi all, For the past several months, I have been attempting to get double immunofluorescence with two goat anti-mouse antibodies on mouse FFPE bone. The antibody that is giving me a lot of trouble is the goat anti-VE-Cadherin from R&D Systems. Essentially, it seems to work without the tyramide but

Re: [Histonet] need tips for cross-sectioning of cortical bone

Hi, All, I primarily section bone, and it's usually paraffin. I second the vote on chilling the blocks (I chill mine in ice water instead of the freezer, so if I forget my blocks the wax doesn't crack!), and using ice water on a swab to keep the block chilled as you section. Generally I secti

RE: [Histonet] ER clone 1D5 or SP1 ?

We use the SP1 clone here. Mark Turner, HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mtur...@carisdx.com ___ His

RE: [Histonet] ER clone 1D5 or SP1 ?

SP-1 here as well Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, April 23, 2010 10:17 AM To: 'ih...@googleg

[Histonet] disposable blade holder

Oh Budget cuts again! I been checking the web for a used disposable blade holder for the Microm HM 310 Microtome with no luck. Does anyone have an extra one laying around that I can purchase cheaply? . ___ Histonet mailing list Histonet@lists.utsouthwest

[Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ?

SP1 On Fri, Apr 23, 2010 at 8:17 AM, Taylor, Jean wrote: > > > I’m wondering which clone of ER most labs are using? > > > > Thanks, > > Jean Taylor, HT(ASCP)QIHC > > IHC Tech > > Meriter Health Services > > Madison, WI > ___ Histonet mailing list Histo

Re: [Histonet] ER clone 1D5 or SP1 ?

Jean: We using clone 1D5 (Dako) since 2008 year with very good results. Maxim Peshkov, Russia, Taganrog. mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/li

RE: [Histonet] new er pr cap guidelines

In those situations you can set up your processors to hold in formalin until the time limit is reached and then switch to 70% Sample comes in on Friday. Place it in formalin (on the processor) until the 48 hours have been reached most likely Sunday afternoon, then switch to an extended 70% alco

RE: [Histonet] ER clone 1D5 or SP1 ?

We are using 6F11... Sally -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, April 23, 2010 10:17 AM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [H

[Histonet] ER clone 1D5 or SP1 ?

I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] new er pr cap guidelines

Yes, since there is still a difference between ER/Pr and Her2 recommended fixation times (ER/Pr at 72, Her2 at 48 hours) the defacto max time for ER/Pr is still 48 hours. They say they are studying the issue Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA --

RE: [Histonet] new er pr cap guidelines

My problem is that we do not work weekends. If I have a breast that comes in on Friday afternoon, I have to cut it in and have it embedded on Sat. morning. If the guidelines are 72 hr for everything then I can process it on Mondays. -Original Message- From: McMahon, Loralee A [mailto:lor

[Histonet] IHC Slides, Hotplate vs Oven

Tony, thanks for your very helpful common-sense approach to answering my questions. It seems that baking time needs to be as standardized, well-controlled and well-documented as other steps in the IHC process. Especially given the importance of standardizing results in predictive and prognost

RE: [Histonet] new er pr cap guidelines

I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix

RE: [Histonet] new er pr cap guidelines

This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only

RE: [Histonet] new er pr cap guidelines

Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has

[Histonet] Positive Controls - IHC

Good day, I am desperately looking for positive controls for Immunohistochemistry testing. I am testing lung tissue for Adenovirus and RSV, but I need a positive control to be able to do this. Since it's not something easily obtained, I tried making cell blocks by using the pellet from a known