From what i know and heard, your facility is definitely getting a great
deal. It really depends on the facility. The lab i work at starts
non-certified techs around 20. I know of a small derm lab that start
non-certified techs around 18.
Emmanuel Gonzaga HT(ASCP)
Tony,
Is this a universal teenage thing, filthy bedrooms? I'm convinced
there are alien life forms in my daughter's bedroom. They make the strangest
noises, grunts and groan and I'm bored. Me, I was the perfect teenager
apart from the Celtic thing of taking to the drink and of course,
If you have one of those great automatic stainers that has a drain tube
going into a sink, you will also find that, after some time, a wonderful
fungus will grow inside of the tubing!! We remove the tubing and shake
the fungus loose. Counter staining isn't the best on this type of
specimen, but
We are a histology lab based in the UK. We have a Leica CV5000
coverslipper and a Leica Autostainer XL.
We are trying to find an independent company or operator (other than
Leica) than can service our Leica equipment for us.
Does anyone know a company or ex Leica engineer we could call?
Brandi,
Body effusions have a high potential for cross contamination during the
staining procedure. Body fluids ie: pleural fluid, peritoneal fluid,
etc. For that reason they are not stained with other non-gyn specimens
ie: bronch. wash, esoph. brush, CSF etc. Also after the staining all
Hello Histo,
Is there anyone out there with any experience of installing a remote voice
dialer on the processor. We have have one and think that it has possibly been
fried due to a power surge. However this one was purchased years ago at Radio
Shack and they no longer sell the same
Good morning,
I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot
of cells along the way (especially when trying to take the cells out of the
tube). We are fixing the cells in NBF, spinning them down, adding 70% and
spinning down again. At that point, I am trying
What are labs using for xylene substitute. I will be using it in a hand
staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3
and formular 83.
Thanks in advance.
Dawn Oakes HT
-
Confidentiality Notice:
Sylinda
Go to www.safemart.com and purchase the visonic ltd model dl-125. It is not
wireless and works wonderful. We have both our processors hooked to it.
Stacey
Sent via BlackBerry from T-Mobile
-Original Message-
From: bsyli...@aol.com bsyli...@aol.com
Date: Wed, 12 May 2010 07:15:18
Whatever you do, do not dry them. Perhaps you could follow the same technique
as cytotechs use to prepare their cell blocks.
René J.
--- On Wed, 5/12/10, Kim Merriam kmerriam2...@yahoo.com wrote:
From: Kim Merriam kmerriam2...@yahoo.com
Subject: [Histonet] (no subject)
To:
Thanks for the tips everyone! I am going to order some agar (histogel) and use
it! We are all very excited around here about this, we have been pulling our
hair out over these darn pellets.
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
From: Cormier,
I know the histogel is pretty expensive (1500ish per kit), try ju= st
plain agar powder and mix with water...it is super cheap that way.
Happy spinning!! =)
Sarah Goebel, B.A., HT (ASCP)
H= istotechnician
XBiotech USA Inc.
8201 East Ri= verside Dr. Bldg 4 Suite 100
=
My histotechs are non-exempt employees. In the state of FL, there is a
licensing difference between HTs and HTLs, and their allowable tasks are
different. There is also a pay scale difference, in our facility, between
the HTs and the HTLs. Starting wage is about $3.00 per hour different.
As
We use Formula 83 and are very happy with it. We use it exclusively in the
VIP processor and to deparaffinize before staining. We also use as clearing
agent after staining, prior to coverslipping, but have found that we still
need the last container before coverslipping to be xylene for best
To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please
contact Master Tech to let them know what you think of the cover.
Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
___
Histonet mailing list
Here's how I do it.
I hate the lens paper method.
I transfer suspended cells from a T75 flask to a 50 ml Falcon tube and
centrifuge at 400 X g for 5 minutes. Aspirate most of the media so that cells
plus media are about 1 ml. Transfer cells plus media to a 1.7 ml
microcentrifuge tube.
We use formula 83 for everything and do not have a problem with a film. We
have found that we must use isopropal alcohol instead of reagent alcohol in the
final dehydration steps.
Margaret Perry
SDSU
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Histonet mailing list
What on earth were they thinking?!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover
We felt offended by the cover also Even my Cytotechs felt that wasn't
necessary!!!
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
This email and any files transmitted with it may
How do we see the cover on line? We did not receive a copy.
Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital Atlanta
terri.br...@northside.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
So how can I see this cover?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Wednesday, May 12, 2010 3:08 PM
To: 'wanda.sm...@hcahealthcare.com'; tgo...@mt.gov;
I had this catalog on my desk and everyone that came in to Histology was
astounded by the cover. No need for the dorsal lithotomy position!
Lynne A. Bell, HT (ASCP)
Technical Specialist, Histology
Central Vermont Medical Center
130 Fisher Road
Barre, VT 05641
802-371-4923
Hello Histonetters,
The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 5, 2010, in Baton Rouge, LA.
Meeting location: The Embassy Suites Hotel
414 Constitution Ave.
Hello Histoland,
I was wondering if anyone out there does stains other than HE 's on specific
tissues. For example, does anyone run stains to rule out H. pylori on all
gastric biopsies? Any information as to the standards in other labs would be
great.
Thanks,
Cristi
My hand staining setup contained some commercial naphta mix, which is
comparable to any other petroleum benzine solvant, for dewaxing.
For dehydrating/coverslipping, I used n-Butyl acetate a.k.a. butyl
ethanoate. Coverslipping can be done with any xylene soluble glue.
What are labs using for
Ah yes,
I remember, actually I don't. It was a bit of a blur!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all
esophageal biopsies with Barrett's indicated by the GI Dr. Our other
special stains are by request, but we almost always end up doing retic and
trichrome on liver bx submitted with hepatitis as Clinical Dx. Hope this
helps.
We routinely do PAS with and without digestion, Trichrome and Iron on liver
biopsies. Some doctors I've worked with preferred an Alcian Yellow/Toluidine
Blue or a Giemsa for H. Pylori. We occasionally did H. Pylori using
immunohistochemistry. We also do Iron stains on all bone marrow smears
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