I have found this helps.
1. Embed the tissue in a dep mould, as this provides more stability, then
2. Face the block
3.. leave in -20 deg freezer overnight
4. remove from freezer and cut sections
5. If you have multiple blocks to work with, leave them in the freezer until
ready to cut
Inexpensive long term anti oxidizing storage? Immerse them in mineral oil!
René J.
--- On Fri, 5/14/10, Morken, Tim timothy.mor...@ucsfmedctr.org wrote:
From: Morken, Tim timothy.mor...@ucsfmedctr.org
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
To: Perry, Samuel
I do this all the time, but I do put a coverslip over the section and keep
it in a humid chamber. We leave it on the counter with a diaper over it and
an ice pack sitting on top.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
Yuk Rene, that sounds so messy, but it is cheap except for tying up racks
and buckets.
Cheers,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-Original Message-
From:
Hello, We have done a study and found that storage in small zip-loc bags at -20
is an inexpensive and convenient way to store slides. In fact the slides stored
for 5 years at -20 stain better than freshly cut sections from the same blocks.
This was also published recently by others. Dr Rimm at
To store cut sections for use later for IHC I do not bake them to melt the
paraffin, they keep better, melt them just before use. I also store the cut
slides at 4dc. For really precious control blocks, I reseal them with
paraffin (just dip in hot paraffin and then cool) and I do also store the
I will be out of the office starting 05/14/2010 and will not return until
05/18/2010.
I am out sick today. I will reply to your message on Monday.
__
This email has been scanned by the MessageLabs Email Security System.
For
Tanisha Neely HT(ASCP) asks: Are the guidelines for a tech who is
strictly limited to grossing
anatomic pathology specimens different than for those of a full time
histotech? Could a bachelor degree'd person with the right course work
qualify for this position? If so, does anyone have
Sarah Goebel asked about prolonged incubation of primary antibodies on an
IHC.
You can do this, usually without increased background or non-specific
staining, but it is imperative that you validate the procedure in comparison
to the published method that you are using. This will require you to
