Happy Friday!
We recently purchased a cryojane for sectioning frozen human and rat
cartilage. I am having some issues with my cartilage sections. I am using the
4X slides with 2 flashes. The sections look beautiful, until I put them into
buffer to do staining. I am getting folds and the
Dear All,
I have a PFA fixed liver frozen sections and I want to stain for cd45.1,
cd45.2 and f4/80. Can you guys please help me with the protocols for the
staining. I would like to do Immunofluroscence.
Thank you all in advance.
Many Thanks,
Anil Kumar.
Should these slides be retained? If so, how long? Or is it enough just to have
the documentation?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Ellen Yee
Sent: Thursday, June 17, 2010 8:21 PM
To: Laurie
Hi Everyone,
We have some cells that we have processed and embedded in histogel (thanks to
everyone for the protocols that were sent to me a couple of weeks ago).
We are planning to do IHC and ISH on these pellets. Will the histogel
interfere with the ISH procedure at all?
Kim
Kim Merriam,
Histonetters,
I just looked up what a cryojane was, and it's pretty neat!
Does anyone else use this?
The one flaw seems to be that you can only put one section on a slide (or at
least that the way it's depicted here:
http://www.instrumedics.com/cryojanetapetransferprocess.htm )
which makes it
Hello,
I know this might be a little out of the rhelm of histonet but, I thought I
would give it a try.
I am struggling to figure out how a Dermatopathologist is paid? Is he/she
paid per slide? Or is he/she paid a base salary, or both? I would just
really like an idea. Thank you for any help.
Happy Friday to all,
Dako has once again raised their prices to the point that I'm asked to find
another IHC labeling kit that is cheaper. Could those doing IHC please
recommend a kit that is as good as Dako's LSAB2?
Thanks so much.
Roger
Roger Charles
Microbiologist II
PA Veterinary
Emily
You can put more than one section on a slide if you need to, but in our
experience it does not work as nicely as depicted all of the time, it
can be a bit tricky to work with on undecalcifed bone and harder
tissues.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory,
Kim the histogel should not interfere with the IHC staining, I'm not sure about
the ISH
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway
Does this kind of seem unnecessary? What are the applicatio= ns that
would make this Cryo-tape beneficial. I have always used the
paintbrush method which takes 3 seconds, and has always worked
perfect.nbs= p; This looks like it would take several minutes? It
We have a glass coverslipper that is not currently working because we need a
diaphragm for the vacuum pump in it. Our repair people tell us we have to get
it from Japan and it will take eight weeks. Anybody have an idea where we can
get a new or used vacuum pump for this coverslipper? We
Try using 0.9% plain 'ol agar. I use it all the time, it's = cheaper
then the histogel and it works fine with the IHCs. I don't kn= ow
about ISH, but it's a thought (and a super cheap trial?)
= br
Sarah Goebel, B= .A., HT (ASCP)
Histotechnician
= /em
XBiotech
Most pathologists I have worked with (some are specialized as der
mapathologists) work with a group if it's a clinical setting. There
a= re specializations they do to become one type or another (heme
guys, derm g= uys, cyto guys, etc.) The clinical ones I have worked
We use Vectastain kits and they always work well. I don't know if you can
use them for your particular IHC but here's their site
www.vectorlabs.com
Emily
Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
On Fri, Jun
I am still struggling when I have a spare minute to get our inherited JB-4
Sorvall manual microtome to work for paraffin sectioning. Now the micron knob
moves freely and I can't get it to stay set on the thickness. If I get it on
say 5 microns as you are cutting it drifts so you never know what
I don't think I can do this with the automated system we are currently
using. Ventana. Does any other Ventana users know if you can do this in
parallel
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
I've used it for serial frozen sections in the past; worked very well
for that application.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From:
I do this but it has to be separate runs or wait until the kit is just running
out. I also keep the slides from the initial QC run and compare it with the new
lot to assure same intensity staining is obtained when changing lots.
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical
You'll have to use prep kit stickers and duplicate protocols to do it, but
it is possible. You just need to copy the protocol and save it as another
number, then change the primary antibody to a prep kit sticker save again
and then put that sticker on the dispenser. Then you need to print
Any pros/cons with leica cm1950 against thermofisher hm550/fumigation option
with cryostats? Two to four days a week using cryostat. Durability issues
Thanks and enjoy the weekend.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
I think that CAP means that you need to save the slide that you ran from the
previous lot and compare it to the slide that you have stained with the new lot
number. To see if they are sufficient diagnostic quality. Not put both lot
numbers on the machine at the same time and then compare the
The Leica Bond detection kits are better than LSAB. They are barcoded for the
Bondmax machine-not sure if they offer packaged another way.
Joanne Mauger HT(ASCP)QIHC
Children's Hospital of Philadelphia
From: histonet-boun...@lists.utsouthwestern.edu
I'm kind of getting in on the middle of this but is anybody else doing this
with prep kit stickers? I have not seen the new question but has anyone talked
to CAP to get a clarification of what they mean on this question?
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical
Mark,
Did you notice the credentials from this CAP representative? MT with a
Blood Bank specialty I believe. What I glean from that is...more than
likely this person does not grasp the logistics of contemporaneously
staining identical Abs from separate lots. She also likely does not
understand
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