RE: [Histonet] Help! In need of positive Gram Control

Take some fresh tissue (we use umbilical cord) to micro and let them incubate in for a few days in gram + and/or negative. Then fix it and you will have good controls. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

RE: [Histonet] Retaining empty jars

We also retain ours; for about 2 weeks. I have plastic bins labeled Monday 1/ Monday 2.. ect.. that way I don't have to search through all of them if I have a question about a case. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503

[Histonet] Prefilled Formalin Jars

Hi histonetters: Was wondering if anybody knew of a vendor that had prefilled formalin jars that have a seal on them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager

RE: [Histonet] (no subject)

Rhonda, We keep our empty biopsy containers for one week and then dispose of them in a red bag. There are no recommendations that I know of, but we started doing it because there was a mix up with a biopsy case and we couldn't figure out if the nurse had put the specimens in the wrong labeled

RE: [Histonet] Retaining empty containers

We keep all of our empty containers for one week in plastic biohazard buckets and we label them Monday, Tuesday, etc. (same as Rosa). Saving them has saved our butt more than a few times! Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road

[Histonet] Breezy lab

We solved the air vent problem by first taping a file folder to the overhead vent so it directed the flow away from our cutting stations. That worked somewhat, but not completely. So we then taped a file folder to the inside of the grate over the vent and now - no more breeze! But i can't wait

[Histonet] Help Resurrecting Dried Fixed Tissue

I am grasping at straws and hoping for ideas to salvage some formalin fixed tissue specimens. I work in a research lab, and am quite new to histology and this particular lab. A former student left formalin fixed tissue specimens that were stored in 70% ethanol. Unfortunately, the seal failed

[Histonet] pancreatin

Hello histonetters! I'm trying to make a pancreatin solution from Sigma pancreatin. There is no information with the actual product however, and the protocols we're following are vague about what to dissolve it in. They say to use a balanced salt solution without Ca 2+ and Mg 2+. We assumed

RE: [Histonet] Retaining empty containers

We too save our containers; on several occasions we had the investigator question the coding (usually their fault)! We keep ours for @a month (overkill), and then empty the formalin into waste container for pickup and discard the bottles. -Original Message- From:

[Histonet] RE:Empty specimen containers

We retain our empty containers for 2 weeks post-final report. D. Goodwin SJGI Marlton, NJ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu

RE: [Histonet] Retaining empty jars

CAP retention guidelines say that wet tissues are to be retained for 2 weeks after final signout. We keep all containers, empty or not, for the 2 weeks then strain and discard them in biohazard trash. We have a large cabinet and designate a shelf for each day. Tom McNemar, HT(ASCP) Histology

RE: [Histonet] Prefilled Formalin Jars

Richard Allen (now available via Fisher Sci) carries pre-filled bottles. I get the 15ml, 30ml, 60ml, 120ml and 180ml. Oh wait, I just read more clearly, these don't have seals on them, just a screw-top lid. Not sure that helps you. :O( Michelle -Original Message- From:

[Histonet] RE: Help Resurrecting Dried Fixed Tissue

I once took tissue that had dried up after being fixed in formalin and soaked it in normal saline to rehydrate it. Very small piece of skin that I soaked for about 3 hours. Then placed the tissue back into formalin and reprocessed routinely. It did not have the best morphology but is was

RE: [Histonet] New CAP question ANP.22760

Joe, You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ... Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience

RE: [Histonet] Retaining empty jars

Ditto. Claire re: CAP retention guidelines say that wet tissues are to be retained for 2 weeks after final signout. We keep all containers, empty or not, for the 2 weeks then strain and discard them in biohazard trash. ___ Histonet mailing list

RE: [Histonet] Retaining empty containers

We also keep the empty containers. The empty containers are kept one week, placed in hazardous bags and labeled by grosser/date, the formalin is emptied, at time of grossing, into our Formalin refiltering system (Creative Waste Solutions) and the recovered formalin is used at the grossing

RE: [Histonet] Prefilled Formalin Jars

I am wondering why the need for a seal? William DeSalvo, B.S., HTL(ASCP) From: sbald...@mhhcc.org To: histonet@lists.utsouthwestern.edu Date: Wed, 23 Jun 2010 09:34:44 -0400 Subject: [Histonet] Prefilled Formalin Jars Hi histonetters: Was wondering if anybody knew of a vendor that

RE: [Histonet] slim jims

We put them in the formalin first just like a specimen. Josie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Tuesday, June 22, 2010 2:48 PM To:

RE: [Histonet] Help! In need of positive Gram Control

It's true! Who knew that Gram + and- riddles meat sticks could be so yummy and versatile too! Josie -Original Message- From: Gill, Caula A. [mailto:cg...@marylandgeneral.org] Sent: Tuesday, June 22, 2010 10:16 AM To: Josie Britton; dianar...@aol.com;

RE: [Histonet] Retaining empty jars

We keep our empty containers for 6 weeks after grossing, which is typically 5 weeks after signout. Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, June 23, 2010

[Histonet] rehydrating dried out tissue and gram positive control(forget the slim jim)

I worked at AFIP for 16 years and worked in infectious disease for 6 and we never used a slim jim. People discussed it but the truth is there was literally thousands of gram positive samples to use, why would we have wanted to use a slim jim? Use appendix that has been tested. Dried out

[Histonet] Pap sign out

We have always had our Pathologist sign out all Pap results, and are now considering having out Cytotech sign out Pap's. Is there an agency we have to report o before we start this process. Also, how are others choosing the 10% for review by the Pathologist? Thanks Gary

[Histonet] Special stain for neutrophils

Hello my Histonet friends, Is there any special stain that you know of to demonstrate neutrophils and/or macrophages in tissue sections? Best, Cathy Cathy M. MathisHistology Core Technician Wake Forest Institute for Regenerative Medicine Wake Forest University Health Sciences Medical Center Blvd.

[Histonet] mouse BBB endothelial marker?

Dear everyone, Does anybody know a good primary antibody for the mouse BBB endothelial staining? Preferably mouse or rat so I can do double with a rabbit antibody, but it is not a big problem. Please let me know if you know a good marker. Thank you in advance, Suh

RE: [Histonet] (no subject)

We retain them until the specimen is signed out, usually no more than 3 days. This has been helpful if the specimen container labeling is called into question either by the pathologist (because the cellular profile does not match what the specimen source indicates) or the clinician. Steve

[Histonet] IHC for Legionella?

Hi, I am not a big fan of Santa Cruz antibodies, but they have a fairly big list of micro-organism antibodies in their catalog. Here is a link to a list of Legionella antibodies from their website. http://www.scbt.com/table-legionella_pneumophila.html You may find what you need there. I

RE: [Histonet] Help Resurrecting Dried Fixed Tissue

We have success with Sandison Method, see below: Sandison Method Solutions: 95% ethanol 300ml 38% Formalin10ml Sodium carbonate10g Water 690ml Method: Leave tissues in solution overnight or until they become soft.

[Histonet] RE: X-gal staining troubleshooting

Hi, Teri, I have seen it in some of my stains, but they were always in embryos of mice from a particular strain (that we did not generate and have incomplete construct information for). I always attributed it to a different LacZ allele, as I have stained litters of a different strain