RE: [Histonet] assisting with patient bone marrows

Everyplace I have worked it was done by hematology. When hemo tried to dump this job on us the pathologist stepped in to remind them that this is their area of expertise. We do not expect hemo to do frozen sections and we do not do bone marrows. After they have made their smears and sent the

[Histonet] Resin embedded tissue

Hi, Does anyone have experience of cutting resin embedded bone and if so what type of slides do people use? We are having problems with sections falling off or the bone not remaining flat on the slide! Many thanks in advance, Val ___ Histonet

Re: [Histonet] Resin embedded tissue

Valerie, I use just regular glass slides, coat them with Haupt's adhesive, roll the sections flat and then press and dry the sections to the coated slide using a slide press and oven at 50C. In 13 years of hard tissue resin histology, I have never (knocking twice on wood) lost a section or had

Re: [Histonet] assisting with patient bone marrows

Oh my - how this makes me smile poor Hema are SUCH busy ones and we in Histo sit around just longing to be able to participate in the 'real' action clinical stuff we are just the dumping ground for all the crap (no disrespect intended to patient samples) that 'others' dont feel like doing. We even

[Histonet] FW: Fried biopsies

From: Carol Fields Sent: Thursday, July 15, 2010 9:37 AM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: Fried biopsies Hi Histotechs, We have fried GI biopsies this morning and I remember a solution you can soak them in to reprocess them and hopefully make them diagnosable. It has

[Histonet] WASTE DISPOSAL

Histonetters I would like to know about this as well! Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only.

RE: [Histonet] Excluded and Limited Tissues to Histo

I believe this list is generated by your surgical affairs medical staff. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:

RE: [Histonet] FW: Fried biopsies

This is a procedure that I have used in the past and it has worked very well. Good luck! Kaye -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Thursday, July 15, 2010 9:43 AM To:

[Histonet] frozen lung slides

Hi all Is anyone of you able to prepare frozen lung slides (7 um) using formalin fixed tissue for IHC? Joost Bruijntjes TNO.NL http://www.tno.nl/ Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijnt...@tno.nl mailto:joost.bruijnt...@tno.nl Disclaimer

RE: [Histonet] FW: Fried biopsies

Sorry, I forgot about attachments. Here is the procedure. REPROCESSING DRIED OR SUB-OPTIMAL TISSUE SPECIMENS PURPOSE: This procedure provides instructions for rehydrating dried tissue specimens. PRINCIPLE: Tissue may become dry due to a malfunctioning of the processor, or if the tissue has

[Histonet] inking dyes

Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain

[Histonet] human on human IHC

Hi, Does anyone know of a human-on-human IHC kit similar to the mouse-on-mouse kits that are available? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: inking dyes

Statlab or TBS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, July 15, 2010 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inking dyes Can someone provide

Re: [Histonet] inking dyes

Try the EMS website I find them useful and their pricing is competative. On Thu, Jul 15, 2010 at 10:52 AM, Diana McCaig dmcc...@ckha.on.ca wrote: Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health

RE: [Histonet] human on human IHC

I am doing alot with this right now. The best thing I have= found to do is to conjugate your primary antibody with HRP, then just use the direct method (no secondary), and it turns out beautifully!! You can buy the= kit to do this from Fisher. I can get the exact

FW: [Histonet] FW: Fried biopsies

Can we please add this to the HistoNet Archives so we can all find this procedure? See attached Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@northside.com -Original Message- From: Kaye Ryan

[Histonet] Histotech opportunities in NC

Histotech positions open at Cape Fear Valley Health System in Fayetteville, NC. Please contact me for more information or apply on the hospitals webpage. Thank you, Jason Keller Technical Specialist Cape Fear Valley Health System Fayetteville, NC CONFIDENTIALITY NOTICE: This

[Histonet] Hemoglobin Stain

Hi all, In my quest for advice about what will be my very first attempt at histology, I have been recommended to post my query here. I'm attempting to demonstrate hemoglobin in FFPE lymph node sections. I've been able to find a couple of techniques (namely the Dunn Thompson technique and

RE: [Histonet] Hemoglobin Stain

Anna, Are you limited to histochemical stains? Because if you're not, hemoglobin may be detected by immunohistochemistry quite nicely. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original

[Histonet] RE: inking dyes

Davidson Marking Systems From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flna...@texaschildrens.org] Sent: Thursday, July 15, 2010 11:46 AM To: 'Diana McCaig';

[Histonet] Wax removal

Hi everyone; This may seem like a insignificant question, but we consistently have issues with injuries to our maintenance staff from cleaning the wax off of our floors. Housekeeping has laid sticky layered mats all over our floors (like large mouse traps), which they peel off layer by layer as

RE: [Histonet] Wax removal

We have a paraffin scraper that our Housekeeping staff uses. It was purchased from American MasterTech Scientific (item CPW04200E, and replacement blades item CPW04201P). We also have perforated anti-fatigue mats in aisles around the cutting stations which trap the wax and can be

[Histonet] Water Testing

Does anyone know if the following are the current CAP requirements for water testing? The following specification for CLRW (Clinical Laboratory Reagent Water) should be met at time of in-house production: CLRW Maximum microbial content (CFU/mL) 10 Minimum resistivity (megohm-cm)

[Histonet] Can anyone use some reagent?

Hello histonet. I have some Dako reagets that we do not nee= d anymore. We have switched from the LSAB2 system to the Flex polymer and have 2 unopened containers of dilutent (S0809) and one unopened contai= ner of TRS (S1699) that we can not use. If anyone wants to

[Histonet] RE: inking dyes

We use Thermo Fisher ones from Fisher HealthCare. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Thursday, July 15, 2010 11:45 AM To: Nails, Felton; 'Diana McCaig';

[Histonet] Histology Job in WI

MPath Search Partners is looking for a Histotechncian/Histotechnologist candidate for permanent direct hire in Wisconsin. *Position: *histotechnician/histotechnologist *Location: *state of the art laboratory in Manitowoc, Wisconsin *Requirements:* HT/HTL ASCP preferred Degree

[Histonet] histotechnician needed

Panama City, Florida is seeking a Histotechnician for growing dermatology practice.Responsibilities include grossing, processing, embedding, cutting, staining, and cover-slipping of skin specimens. HT (ASCP) certification preferred, no Florida license

[Histonet] RE: thanks

Thanks to all who answered about mouse macrophages!. I will try F4/80. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth

[Histonet] Robert D Kobus is out of the Laboratory.

I will be out of the office starting Thu 07/15/2010 and will not return until Mon 07/19/2010. I will be out of the Laboratory from Friday 7/15/2010 until 7/19/2010. If you have a matter that can not wait, please page me at 202-801-4851 or call my cell phone at 301-832-7626. Otherwise I will

Re: [Histonet] assisting with patient bone marrows

Amen! said in my best Rodney Dangerfield voice Can't get no respect, I tell ya! Just no respect! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Anne van Binsbergen anni...@gmail.com Sent by:

[Histonet] NG2 antibody/ Antigen retrieval/Protocol

Hello, I am trying to stain human glioma sections(as a positive control) using Anti-NG2 antibody. The tissue that I have is Paraffin embedded and I appreciate if somebody could send me the protocol for antigen retrieval ,antibody titer and IHC protocol. Thanks, Maryam

RE: [Histonet] Wax removal

Hi Rhonda, Where do you get the anti-fatigue mats Thanks. Tom Tom Wells BSc, ART Faculty School of Medical Laboratory Sciences British Columbia Institute of Technology Burnaby, BC Canada -histonet-boun...@lists.utsouthwestern.edu wrote: - To: Hartz, Rhonda SktnHR

[Histonet] Re: inking dyes

Diana McCaig in Chatham, Ontario asks Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. You're referring to inks used to mark the margins of resected tissue so that the pathologist can find the surgical margins under the microscope. They're particulate