RE: [Histonet] Formalin substitutes

The following info might be useful: It is believed that formalin given time will kill any microorganisms that are present in tissue and that formalin will inactivate tuberculosis. Vardaxis et al (J Clin Pathol 1997;50:429-433) were quite rightly concerned with the disinfection of bacterial

[Histonet] Reaction/s between Phloroglucinol and Toluidine Blue ?

Hello Histonetters, I have been staining residues with Phloroglucinol which is light sensitive however I due to time constraints I have not been able to wait for all the colour to fade ( as this takes about one week). Therefore, I have dried off the stained residue under lights and then

[Histonet] Job Opportunity

Hey histonetters!   Keiser University is looking for a program coordinator for their histotechnology program at their Pembroke Pines campus.  For further information please contact   Tania Phillips, MBA Associate Dean - Allied Health Keiser University - Pembroke Pines 12520 Pines Boulevard

[Histonet] Brain Problems

Hi there, I am currently using rat brain for histology but something is not working. I follow the protocol below for tissue processing. Station Solution Routine (mins) 1 Formalin 10 2 Formalin 10 3 70% Ethanol 60 4 80% Ethanol 240 5 96% Ethanol 240 6 100%

[Histonet] RE: Brain Problems

How big are these rat brains? Are you processing by hand or with a machine? We routinely process pieces of human brain for about 10 to 11 hours on a VIP. This seems like a really really long cycle. No heat and no vacuum until the paraffin infiltration step. (formalin, 70% EtoH, 95% EtoH,

RE: [Histonet] Brain Problems

Try cooling down your water bath with ice before you cut the brai= n. All CNS tissue will act like you described. You may end up w= ith more wrinkles with the cooler bath, but put the slides in a 70C oven fo= r about an hour and they will usually fall out. =) Happy Cuttin=

[Histonet] Re: [Sectioning] Brain Tissue

To All: We have been experiencing problems sectioning mouse brain. The sectioning is fine, but we have problems in the water bath. At 45 degrees C (peel-a-way paraffin-Polysciences), the sections break apart but we don't get wrinkles. With paraplast extra, we use 36 degrees C, but the sections

[Histonet] Re: [Sectioning] Brain Tissue

We keep our water bath at 40-41 C. Sometimes we add some 70% ethanol to the water bath and this helps to get the wrinkles out (especially for cerebellum and mice brain). Most of our tissue is bovine brain but we deal with multiple other species including mice. We use McCormick Paraplast Plus

Re: [Histonet] Powdered reagent expiration dates

Everyone, Thanks for your help, based off your explanations, statements and citations, we will attempt to protest this and have it expunged. On Mon, Jul 26, 2010 at 4:41 PM, Lee Peggy Wenk lpw...@sbcglobal.netwrote: See if you can get the following article. Biotech Histochem is published by

[Histonet] Tissue processing protocol for Hamster tissues

Dear Histonetters, We were asked to process Hamster tissue(Large intestine) for one of our investigators. I would like to know whether any of our users process hamster tissues in their labs, if so kindly provide me a protocol for the same. Our tissue processor is Sakura VIP6. Thanks for all

[Histonet] question for a friend

A friend is asking this question and we were looking for responses telling what others would do in this situation: I am hoping to submit some brains for paraffin-embedded sectioning soon, but we started intending to go the frozen-section route, so I have some questions as to the

[Histonet] Using GEWF solution and IHC staining

At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how safe it is to use when staining for IHC. Does anyone have

Re: [Histonet] Using GEWF solution and IHC staining

Sounds like to me that it might involve another revalidation of each one of your antibodies. Good luck On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) randi.ha...@horizonnb.ca wrote: At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled

[Histonet] Great New Position In Irving, Texas

Nueterra, a leading developer and manager of physician-owned surgery centers and specialty hospitals has an immediate opportunity available for a Histotechnician. Great opportunity for a Histotechnician in a brand new laboratory! Nueterra Pathology Services in Irving, TX is looking for a

[Histonet] Ketamine Supplier

I was wondering if anyone could help me find a supplier for Ketamine. We usually order ours from Sigma but they are back ordered until mid September. I have considered trying to order Ketamine/Xylazine solution in place of the powdered forms but I don't know what ratio to get. We use this

Re: [Histonet] Using GEWF solution and IHC staining

Ethanol/alcohol is what will process the specimen. If the tissue is fixed would it really matter that the tissue came into contact with ethanol? Mark On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) randi.ha...@horizonnb.ca wrote: At a recent conference, our PA learned of using GEWF

[Histonet] Re: how to isolate scabs

Hello all, I have received embolic material in test tubes in 10% formalin. The tubes had been sitting around for about 2 years. To quantitate the number of embolic particles, after removing particles at less than 40 microns, the solution was poured into a petri dish and

RE: [Histonet] Brain Problems

The fixation time is too short. Fix as long as you can (how about 2-3 days at least for brain?) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and