I am wondering how much agitation is required in order to
achieve the clearing of paraffin from the tissue and slide.
IHC systems obviously use adhesive or + slides, aiding in
tissue adherence. Too much agitation might take take tissue
off. And what about nail fragments or hard tissues in
general?
I recently had an enquiry from one of our post docs who was looking at TUNEL
staining and came across this reference in biochemica no 4 (1997)titled
“Fixation of Tissue Sections for
TUNEL Combined with Staining for
Thymic Epithelial Cell Marker”
You should be able to google it and find the