Hi,
What concentration of HCL are you using in solution B ?
is it 1% in the end, so i will have to use 3ml of the standard 36% HCL in
100ml, not just 1ml as mentioned in the protocols ?
Itai
*gayle callis** **gayle.callis @t bresnan.net
Good Morning All,
I am having some issues when cutting a microarray containing 4mm punches.
The punches are rolling and separating from the paraffin during cutting.
Anyone have any tips or ideas??
Rae Staskiewicz
MMCI
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Whilst value for money and costings rule then the management will
always look to save $$$s by hiring semi/unskilled
personnel.the other point in this thread is that
people are increasingly using the Histonet as an on-line and up to
date text,
hi, this sounds like the tissue was too cool when embedded in the wax - so
it has not made a nice homogenous block that cuts as one. Can u re-embed?
On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz raest...@grics.netwrote:
Good Morning All,
I am having some issues when cutting a
Hello, We have trouble with the larger size punches as well. In order to get
the cores to stick to the size try warming the block in a oven at 37 degrees
for 15 minutes, put the block face down on a clean slide. (providing the
melting temperature of the paraffin is about 57 degrees). After 15
Hello, We have trouble with the larger size punches as well. In order to get
the cores to stick to the size try warming the block in a oven at 37 degrees
for 15 minutes, put the block face down on a clean slide. (providing the
melting temperature of the paraffin is about 57 degrees). After 15
We use Weigert's for 7 minutes and never more than a week either. As a
precaution we keep it in the dark in a non-transparent container. That's the
way I was always taught (26 years and counting)
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710
Agreed.
--On Saturday, September 18, 2010 1:25 PM -0400 Carrie Disbrow
cdisb...@msn.com wrote:
As a newbie trying to learn as much as possible, I'm disappointed when
questions are posted and the responses are sent to the individual. I
learn more when the responses are shared. Also, an
First off you shouldn't leave Bouin's sitting for that long out o= n a
shelf. Once the water evaporates out of the solution it is extrem ely
flammable (explosive even I think?). Also, you shouldn't leave
ti= ssue in alcohol forever either because you will end up with beef
Hi All,
Our private derm-path lab, located in central-western New York State, has an
immediate opening for a full time histotech to work days. A relocation bonus
is negotiable.
To learn more please contact me directly.
Joyce
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We have three CBG recyclers - two are used for formalin and one is used for
alcohol/xylene. They run every day and we have very little trouble with them.
Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA
--
-Original Message-
From: Dawson, Glen
Sent: Monday, September 20, 2010 9:10 AM
To: 'Merced M Leiker'
Subject:RE: [Histonet] Posting comments
Carrie,
Until you've had some psychopath threaten you with a lawsuit, or threaten to
tell on you with your current employer,
I will be out the office the week of September 20-24.
I will return on Monday, September 27th.
If you need laboratory assistance please call 785-273-2788 ext. 322.
Thanks.
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I originally asked my questions because I *knew* it was being done
incorrectly and no one @ my workplace believed me when I tried to show
them the way I was taught/trained-as stated in my original post.
Regardless, it never occurred to me that my questions were something
that would be met with oh
James,
Well said. I totally agree
Thank you very much!
Amy
Message: 4
Date: Fri, 17 Sep 2010 11:54:42 -0600
From: james leroux jler...@petroglyphpath.com
Subject: RE: [Histonet] RE: Cutting, Processing, etc
To: 'Nails, Felton' flna...@texaschildrens.org,
Amy,
You do not need to apologize for asking a question to which you did not know
the answer. This is an educational avenue, for histology, and there is no such
thing as a stupid question if you need answers to solve a problem.
Those of us who teach know questions are important, even if
Agreed!
Thanks,
Timothy Higgins, HT(ASCP) QIHC
Histology Manager
APA
Amarillo, TX
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A.
Powell
Sent: Monday, September 20, 2010 10:33 AM
To: Senn, Amy
Shirley and Amy and others responding to this thread: thanks for your posts.
I am not in the histotechnology field - I'm actually a graduate student at
Loyola who does her own processing, cutting, and staining of bone tissue - so I
really appreciate every so-called dumb question, no matter how
Well said, Shirley!
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
Shirley A. Powell powell...@mercer.edu
Sent by:
We are going to be posting a histology lab assistant position soo= n
for the first time. I would like to give them a skills assessment t est
to see how well they can handle a computer, numbers, problem
solving, a= nd what their handwriting looks like. Does anyone out
Hi everybody!
Does anyone know where I can purchase a multi-timer (product used to be
manufactured by Beckman Coulter electronics)? The company no longer
manufactures them. Thank you so much!
Paula Wilder
St. Joseph Medical Center
410-337-1741
Try Labsco.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Wilder
Sent: Monday, September 20, 2010 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] multi-timer
Hi everybody!
Does
I would like to know if anyone has a good protocol that includes tissue
fixation (preferred fixative, fixation temperature etc) and tissue
processing (time etc.) for low melting Steedman polyester wax . The
tissue to be embedded is chick embryo brain.
I am going to do all the tissue
Hey guys
Was wondering if any of you ever used a phopho NFKB p65 antibodu and which
one you prefer with what antigen retrieval method if applies.
I'm working on FFPE rat tissue
Thanks.
Fabrice
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