[Histonet] Direct immunofluorescence question

Barb, Have you tried a diamond pencil? Available from a variety of sources, these pencils can be used to etch a circle or other shape around the tissue to be viewed. I believe they use lesser-quality industrial diamonds to make the tips on the pencils (i.e. not the diamond ring quality).

[Histonet] Rio-Hortega Silver Carbonate Stain Polak's variant?

Hi All, Question - Has anyone heard of a Rio-Hortega Silver Carbonate stain (Polak's variant) for mitochondria staining? Any suggestions? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk

RE: [Histonet] Fibronectin AB for human tissues

I use AbCam 32419 (rabbit monoclonal, clone F1) on the Bond at a dilution of 1:300 after EDTA retrieval; very clean, crisp results Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From:

[Histonet] Re: Histonet Digest, Vol 83, Issue 4

Barb, Eric, all- We also use a diamond pen/pencil-and they do contain an industrial grade diamond, so they are quite reasonably priced; I looked one up on the VWR website (just as a reference, not necessarily an endorsement! :o) and they run about $20-30 each. They last a very long time,

[Histonet] Rabbit Brain Tissue Processing Schedule

Hello everyone, While I am well versed in protocols to process bone from a variety of species, my experience in processing soft tissue is less extensive. Currently, I do have some soft tissue protocols which I inherited from my predecessor, and with which I have generally had success, but I

[Histonet] Re: Rio Hortega (Polaks)

Patsy, Here is THE citation and abstract for this publication in J Histochem Cytochem (found it by Googling staining method). You can download the pdf at no charge. The photographs are excellent. Journal of Histochemistry and Cytochemistry Volume 52 (2): 211-216, 2004 Silver

[Histonet] Osteoclast

We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458

Re: [Histonet] Osteoclast

You can do TRAP ( Tartrate-resistant Acidic Phosphatase) stain for osteoclasts. On 10/5/10 4:58 PM, Lin Bustamante lbustama...@cvm.tamu.edu wrote: We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate

[Histonet] embedding beads/tissue marking dyes

Hi We may be encountering some issues with our tissue marking dyes 'clogging' up our processors. Ive heard of a system where a coloured 'bead' is embedded right beside the tissue, and subsequently cut onto the slide. This does not mark the margins obviously, but is used as a method to track

[Histonet] RE: embedding beads/tissue marking dyes

When you place dye on any tissue, make sure you pipette some bouins on to the tissue... Apparently the bouins sets the stain... afterwards dab the tissue with paper towel to sop up the excess dye/bouins. Some dyes are better than others, but try the bouins first Call me or email me directly, I

RE: [Histonet] DIF Transport Media

Vinnie, I hope you are well. The following might be of use: Specimens for immunofluorescence are usually submitted to the laboratory in cell culture fluid (eg Hanks or RPMI) or on saline soaked gauze. For transport to other institutions, Michel's Transport Medium has often been used: