Laurie - Tru Sigma/Aldrich. I used to get mine from them.
Cheryl
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I was wondering if anyone is using this enzyme. If so, where do you buy it?
Is it extremely expensive? Could you share your procedure for preparation?
Janet
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Sorry, I was referring to glycogen digestion and PAS staining.
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Does anyone have a transport/ carrier for transporting amputations.? We
have specimens funneled into one site for grossing, and need to
transport amputations AK and BK. Thanks for any help!
This message contains information that is confidential and may be privileged.
Unless you are the
Hi Histonetters!
We have someone retiring soon and will have a position for a histotech
coming up.
We do about 12,000 cases a year( 50,000 or so blocks). We have a Benchmark
XT IHC, Dako special stainer and we are in the middle of getting a Vantage
tracking system. We are like family here,
Try mopec
-Original Message-
From: Fleming, Jackie M jackie.flem...@allina.com
Sent: Wednesday, October 13, 2010 7:59 AM
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amputation transport
Does anyone have a transport/ carrier for
Good Morning to All,
We have an off site hospital and the courier has a large deep plastic storage
bin, like you get at Walmart, that he brings the amputations in. It is a blue
bin (of course, not clear). It works very well.
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT
We run a weekend (Friday til Monday AM) breast run where the tissues are in
10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete
processing on Monday morning. So far no problems.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
How long are you pre-processing the prostate biopsies before processing on the
XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours,
then wash for 20 minutes, pre-processing solution for 10 minutes then process.
We use Harris Hematoxylin in our HE and morphology is good.
Phyllis:
The time required in NBF is not 8 hours only, that is exactly what the
regulations are trying to avoid, i.e. incomplete fixation.
René J.
--- On Wed, 10/13/10, Phyllis Thaxton dch...@yahoo.com wrote:
From: Phyllis Thaxton dch...@yahoo.com
Subject: Re: [Histonet] RE: New Cap Guidelines
Hey Histonetters. How about all of us getting involved in providing
some input to the Joint Commission on their accreditation standards with
regard to Histopathology. Apparently, there has not been a great
response from the histology community to this request. This is our
opportunity to impact
Have you validated this processing? Leaving the tissue in 70% alcohol for 48
hours is not standard, and thus requires all of the hassles associated with
validation.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido,
For the weekend, we have our processor set for 36 hours in formalin and
then a hold in 70%. This allows for complete fixation and cuts down on
prolonged time in 70%
From: Phyllis Thaxton [mailto:dch...@yahoo.com]
Sent: Wednesday, October 13, 2010 10:32 AM
To:
Hopefully, ASCO and CAP will relax their fixation guidelines for HER2 testing
in the near future to reflect that prolonged fixation in formalin has little,
if any, affect on HER2 protein overexpression (my personal experience).
Another article (see below) just appeared in the October issue of
Just Google: nitroblue tetraqzonium chloride. Lots of sources.
Cheers,
Fred Monson
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Tuesday, October 12, 2010 6:41 PM
To:
How much time is the tissue in formalin prior to going in the processor?
Your total time in formalin can not exceed 48 hr. And you will still
need to validate your process if you hold your tissue longer than
normal processing time in 70% (ie. more than 1 hr).
-Original Message-
OK I usually do not like to chime in on this, but here I go. How can a
true validation of a specific target be obtained if the wiggle room is 6
to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the
results for this testing if you are going to be varying process for the
weekend vs
Don't hold your breath. I have had multiple conversations with at least
one of the primary individuals who set the standards and i have not
detected ANY willingness to modify the standards and that includes a
hard and fast insistence on good validation for ANYTHING that differs
from standard
Sigma-Aldrich has it.j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
jankeep...@gmail.com
Sent: Wednesday, October 13, 2010 09:35
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Type II-A
If TUR is 88307 and resection is 88309, what CPT code do you use for bladder
diverticuli?
Thanks, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Confidentiality Notice:
This e-mail, including any
Rene,
8 hours is what is on the processor. We keep a log book on all breast tissue.
The OR nurse documents on the label; the time of excision (placed in 10%
NBF) .
The gross assistant documents the time of dissection, Then the time the tissues
are placed on the processor. Our fixation times
Hi Histonet,
I have been trying to work up HCV antibody without much success. Anyone out
there successfully doing this that would like to share their protocol with me?
I have many cases that have been diagnosed with HCV infection, so the control
tissue is not an issue.
We are using the Dako
Is it possible for me to remove the coverslip, run back to water and re-use the
slides starting with the correct secondary?
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410)
If you go to a general store like Fred Meyers, Target...you can get the plastic
containers that hold wrapping paper rolls that are opaque and put appropriate
stickers on them for less than $20.00 I have been using 3 of them for years
now.
Kathy Boozer, HT (ASCP), IHCQ
Adventist Medical Center
Yes
René J.
--- On Wed, 10/13/10, Kimberly Tuttle ktut...@umm.edu wrote:
From: Kimberly Tuttle ktut...@umm.edu
Subject: [Histonet] IHC OOps wrong secondary
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Wednesday, October 13, 2010, 12:07 PM
Is it possible for me
All,
I've been watching this thread for a couple of days now and would like all
to know that had they attended workshop 17, provided they were in Seattle,
it would have been most insightful regarding all these guidelines. It was
titled Quality Biospecimens for Personalized Molecular Healthacare
When you have results from an outside lab, i.e. flow results, bone marrows,
ect. How do you integrate this into your path report?
It is a verbatim copy inside the report in the same format as the outside lab?
Or can it be a copy with the results in a different format? A discussion
has
We are seeking a qualified candidate for the position of Grossing/Lead Histo
Tech in our Chattanooga location. We are a state of art laboratory using the
Ventana Vantage system to maintain specimen integrity; we also have Ventana
special stainers and the Benchmark Ultra and XTs for our
Joyce Weems in Atlanta asks:
If TUR is 88307 and resection is 88309, what CPT code do you use for bladder
diverticuli?
A transurethral resection of the prostate (TURP) is coded 88305 no
matter how many blocks. A TUR of the bladder (presumably a TURBT, that
is, a TUR of a bladder tumor) is
We are not open on weekends and worked out a deal with our core l= ab
that is open 24/7. When we have a breast on the processer we willrun
a Sat. program that ends at 21:00. They added a task on their
dai= ly checklist to come up and take the tissues off at that time.
Fixation prior to the processor will not exce4ed 12 hrs. That is why we
program the processor for 36...not to exceed 48.
From: Mike Pence [mailto:mpe...@grhs.net]
Sent: Wednesday, October 13, 2010 11:04 AM
To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice
If our cases are needle cores they are almost immediately put into formalin
from the patient. That time is recorded by the nurse or the clinician that
took the specimen.
The larger breast samples are received fresh from the OR and are immediately
grossed either by a PA or resident. Those
Hi histoworld,
I would like to repeat my staining on the slides already coverslipped but need
to remove hematoxilin first.
How to remove hematoxilin? Is it need to repeat Antigen retrieval?
Thanks in advance,
Naira
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Thanks for your response, Jose, I agree with your points.
I see these guidelines as the first attempt to standardize something that has
numerous variables. How can we expect to have the manufacturers of these
markers offer (or be required by the FDA) consistent results when our end of
this
To anyone in the San Francisco Bay area, generally northern half: Is anyone
interested in training a neophyte for histotechnology?
A person in San Francisco contacted me about learning histology. He came and
observed in our lab and is very interested but needs a lab to train in. He is
looking
Thanks to everyone who responded. I removed the coverslip, and ran it back to
water and re applied the secondary, dab and counterstain. It worked like a
charm.
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
The date is set, the topics are in place, and the money is just right, all
we needis you to attend.
When: Friday, November 5, 2010
Where: Somerset Medical Center
110 Rehill Ave
Somerville, NJ 08876
*2010 Focus on IHC* presented by NJSH and sponsored by BioCare Medical,
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