[Histonet] High Complexity Testing

Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a machine is doing the work. Can anyone help me out with some guidelines,

RE: [Histonet] High Complexity Testing

Sheila, Yes, IHC/ISH is considered high complexity testing. Sounds like you have a real gem for a pathologist...sorry to hear that. Just don't let him/her pay you like a janitor just because that's what he/she thinks that is what you are. Glen Dawson BS, HT(ASCP) QIHC IHC Manager

RE: [Histonet] High Complexity Testing

Sorry for the early morning grammar everyone... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 7:13 AM To: 'Sheila Fonner'; histonet@lists.utsouthwestern.edu

RE: [Histonet] High Complexity Testing

This is the response I received a year ago from CAP when asked the same question regarding high complexity testing - Immunohistochemistry staining (manual or automated) is considered specimen processing and is not assigned a test complexity Has something changed recently?

[Histonet] (no subject)

Hi guys, For those of you that have been using Thermo nylon biospy bags, what are you doing now that Thermo no longer stocks them? They changed the bags, 2 seams to 3 seams, I tried the new ones, but I don't like them as well as the original ones. I found them hard to get open and several

[Histonet] Re: Histonet Digest, Vol 87, Issue 12

Dear Colleagues, I would like to find chromogen for alkaline phosphatase enzyme labeling. I have used Vulcan fast red from Biocare, but it usually gives me background especially on double stained slides on mouse pancreas, i do KI 67 and BRDU/ Insulin double staining, and  I use AP enzyme for

[Histonet] mart-1 staining in Mohs

Hello, Does anyone out there stain for mart-1 on their melanoma cases on frozen sections. I work for a Mohs surgeon in NYS and we are interested in doing our own testing in our lab for our melanoma cases. We are currently sending out our melanomas for slow Mohs. Any advice would be greatly

[Histonet] mouse skeletal muscle frozen section prep

I am new to working with mouse skeletal muscle and have been reading about various methods for preparing and freezing tissue for sectioning. I have heard of the benefits of coating the tissue in talc prior to freezing but have not yet seen a single protocol that describes the use of talc and

Re: [Histonet] High Complexity Testing

When a machine is doing the test, there are stringent provisions as to the preparation and validations of the test. Done manually, it requires a trained technologists and, yes, they are high complexity tests (both IHC and FISH, and their variations). René J. --- On Tue, 2/8/11, Sheila Fonner

Re: [Histonet] High Complexity Testing

The performance of ISH/IHC is in many laboratories considered high complexity testing - however - as the technologist doing the work does not make the diagnosis it is considered to be a part of specimen processing. (How's that for political double talking!) Use of automation in these procedures

RE: [Histonet] High Complexity Testing

While the test is high complexity it is the READING of the test by the pathologist that determines its complexity. Because histotechs do not report the results our part of this test is not high complexity. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription

[Histonet] RELIA Special Histology Job Alert - Histology Trainer needed in Chicago. Can you help?

Hi Histonetters! I hope everyone is having a great day and staying warm! I have a new position that I am pretty excited about and thought I would run it by you guys and see if anyone was interested or knew of anyone who might be interested. Here are the details: Histology Trainer Full time

Re: [Histonet] High Complexity Testing

That was a hole in 1, Vikki. Viva la rant!! Victoria Baker bakevicto...@gmail.com 2/8/2011 11:58 AM The performance of ISH/IHC is in many laboratories considered high complexity testing - however - as the technologist doing the work does not make the diagnosis it is considered to be a part of

RE: [Histonet] High Complexity Testing

I must disagree with this assessment of what makes a test complex. If the test is done properly [the responsibility of the technologist] then the reading to the test is a visual determination that requires experience on the part of the pathologist, but if the test is not done properly, will

RE: [Histonet] High Complexity Testing

Honey... Estrogen won't do a thing!!! j:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 11:59 To: O'Donnell, Bill Cc: Dawson, Glen;

RE: [Histonet] High Complexity Testing

We were recently inspected and were told the same thing by CAP about 4 months ago. Joe Milano -Original Message- From: Inman, Anna [mailto:anna.in...@stmarygj.org] Sent: Tuesday, February 08, 2011 9:27 AM To: Dawson, Glen; Sheila Fonner; histonet@lists.utsouthwestern.edu Subject: RE:

Re: [Histonet] mouse skeletal muscle frozen section prep

I've frozen numerous rodent (including mouse) skeletal muscle tissue for sectioning. I've never heard of using talc before. I just blot any moisture off the tissue with a paper towel or Kimwipe. I usually do not fix the tissue beforehand but after sectioning, on the slide. To freeze, I just

RE: [Histonet] High Complexity Testing

The CLIA criteria are below. Note that the entire test is considered to be the combination of pre-analytic, analytic and post-analytic. It does not matter how the test is performed (manual or automated) because the FDA determines complexity of a test as sold by a vendor. It goes with the test,

Re: [Histonet] High Complexity Testing

I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a

RE: [Histonet] High Complexity Testing

Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing validation requirements involved if all IHC is is

RE: [Histonet] High Complexity Testing

I completely agree!!! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] High Complexity Testing

Could not have said it better! Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Dawson, Glen

[Histonet] IgG3 negative control

Can anyone tell me where I can buy mouse IgG3 to use as a negative control for some IHC I am doing. I have looked around in my usual places and can't find anyone who has it. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of

[Histonet] Cassette labeling

Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the

[Histonet] Region III symposium - Call for vendors

Hi everyone! The Tennessee Society for Histotechnology will host the Region III symposium in Nashville, TN on April 8-9. If you would like to exhibit at the symposium and need more information, please contact me directly via email or telephone. We offer an unmanned display option for companies

RE: [Histonet] Cassette labeling

B Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew

[Histonet] IHC validation

Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start

Re: [Histonet] High Complexity Testing

I was devastated to learn that IHC is not considered High Complexity Testing. I agree with Bonnie that we do not report results, therefore, IHC is not considered high complexity testing. Sad but true. Somebody pass the Scotch. I'm way past Tylenol JTT - Original Message - From:

RE: [Histonet] IHC validation

Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado

RE: [Histonet] IHC validation

But that is for receptors, correct? Do you do that for everything? Thanks, j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet

RE: [Histonet] IHC validation

Nope that's the recommendation for everything, in the paper they state additional development is required for prognostic markers. Once you have validated an antibody it only requires 3 tissues when you get a new lot number: 1 strong positive, 1 weak to moderate positive and 1 negative. From how I

RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI

Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology

RE: [Histonet] IHC validation

When changing instruments you are validating the instrument, not the test. For each antibody you just need to run parallel tests in each instrument showing equivalence. However, if you change the instrument and ALSO change the antibody and/or detection system, Antigen retrieval, etc, then you

RE: [Histonet] IHC validation

Thanks Tim This is a good way of approaching this. What types of antibodies do you suggest running the reproducibility tests. For reproducibility I run about 3 slides in 3 different runs. I only run reproducibility tests for antibody cross reactivity studies (just after protocol development)

[Histonet] Nickel-DAB Signal Fading

Hello Histonetters! I am once again completely baffled, and thought you might be able to help. I run immunocytochemistry on rodent brain tissue every week, always using the exact same procedures, solutions, buffers, chromagen, and mounting medium. The only variable is the primary and secondary

Re: [Histonet] IHC validation

fortunately, we don't perform prognostic markers. The doctors don't even want to venture down that road. When I started working here, that's the first ting I mentioned. We can save some much money by doing them in-house. That went over like a lead balloon. On a side note, the staff now were

RE: [Histonet] High Complexity Testing

Completely agree with you Glen. However, do take exception to the initial comment on this thread about a machine does all the work. The machine does all the work comment is very telling about what this pathologist (or group of pathologists) knows and does not know about running IHC. If the

RE: [Histonet] IHC validation

Liz: I'd like to know more about these recommendations. Could you provide a journal reference to the paper from CAP? Thx, Sally -- Message: 15 Date: Tue, 8 Feb 2011 16:19:53 -0700 From: Liz Chlipala l...@premierlab.com Subject: RE: [Histonet] IHC validation To:

[Histonet] Fw: Looking for position in Texas

      Hello all, I am looking for a new histotech position in texas, especially in the houston area so as to be,  very close to my family. Though, any part of texas is okay.     I am an HT(ASCP) certified histotech. I do have substancial years of experience and with QIHC(ASCP)