[Histonet] RE: Histonet Digest, Vol 87, Issue 28

Dear everyone, I'm wondering where I can order the Picro-Mallory Trichrome Stain kit? I couldn't find it online excepted some Italian companies. Thanks, Minhui Ma MD,HTL Emory University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

I do not know of a neutrophil specific ab for human ffpe tissue. You could do just DAB without blocking with h202 for myleoperoxidase but it will not distinguish between eosinophils and neutrophils myeloperoxidase will stain all myeloid cells with granules in the cytoplasm (granulocytes). Patsy

Re: [Histonet] Drosophila Sectioning

I have done whiteflies, mosquitoes, honey bees and cockroaches among other insects and insect parts. If you contact me offline maybe I can help you. Andi Grantham On Feb 17, 2011, at 7:46 AM, Gil Sharon wrote: Hi All, As a microbial ecologist, working with drosophila, I'm looking

[Histonet] Zinc Formalin and IHC

Posting for a friend. Replay to shernan...@st-joseph.org Is there any negative effect on IHC and Special Stain with the use of buffered Zinc Formalin? Thank you. Susan Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM

[Histonet] Drosophila sectioning

Hi Gil, My histology service specializes in insects related invertebrates. I have a protocol that I can send you answer any additional questions you may have. Please feel free to contact me. Best, Damien Laudier ___ Histonet mailing list

Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE tissues, according to their website. Jan Shivers - Original Message - From: Patsy Ruegg pru...@ihctech.net To: 'Amy Lee' amylee...@yahoo.com; 'histonet' histonet@lists.utsouthwestern.edu Sent: Thursday, February 17,

[Histonet] I

I will be out of the office starting 02/17/2011 and will not return until 02/21/2011. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. ___

RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

Does it also work on human? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the

[Histonet] liver endothelial stains

What antibody would you recommend using to label the liver endothelial cells? Is factor 8 our best marker? Also do you know of a special stain other than HE that would demonstrate these cells? Thank you. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota

Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

The website says it works on humans. I have not tried this particular Ab myself, though I have tried another xNeutrophil Elastase. Jan Shivers - Original Message - From: Patsy Ruegg pru...@ihctech.net To: 'Jan Shivers' shive...@umn.edu; 'Amy Lee' amylee...@yahoo.com; 'histonet'

.._RE: [Histonet] Zinc Formalin and IHC

We have used Buffered Zinc Formalin for many years and have not had any real problems with IHC or Special Stains. Valerie Hannen Parrish Medical Center Titusville, Florida From: histonet-boun...@lists.utsouthwestern.edu on behalf of Lin Bustamante Sent: Thu

[Histonet] RE: neutrophil Ab for rat FFPE

Amy, We have used Abcam rabbit anti human myeloperoxidase (Ab9535) with very good results on FFPE rat tissue. No retrieval required, primary at 1:50, biotinylated secondary and ABC elite with DAB for detection. Hope that helps. Jason Jason Palmer Histology Laboratory Coordinator O'Brien

[Histonet] Tissue Processors

Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe

RE: [Histonet] Tissue Processors

Joe, We are really impressed with the Leica ASP 300. We have a second one on the way. Stacey People are not an interruption of our business. People are our business. Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120

[Histonet] IHC pretreatment with NaOH/H2O2

I recently came across a protocol that was conducting IHC staining for pSTAT3 on free-floating fixed rat brain sections. The protocol stated that the tissue was placed in 1% NaOH and 1% H2O2 in H2O for 20 min, 0.3% glycine for 10 min, and 0.03% sodium dodecyl sulfate for 10 min. Could someone