Michele,
I would use the expiration dates from the original alcohol bottles for
graded alcohols. Alcohol won't expire any faster just because you dilute
it. I use two years from the month of recycling for an expiration date for
recycled product. In reality these chemicals won't expire for decade
We switched a few years ago. Hospital went mercury free, and we couldn't
find any company in 6 states that would take waste mercury.
One common mistake when converting to a zinc formalin is to try to fix it
the same time as the B5. Mercury binds/fixes very quickly, zinc is slower.
It's chemist
The comments on Verhoeff's hematoxylin should be sufficient to
trouble-shoot this cumbersome but beautiful old stain - I don't know a
stain I'd rather photograph, except maybe Ramón y Cajal's gold
sublimate.
Verhoeff's hematoxylin is a century old this year. The original paper
is in the JAMA, of a
Thanks. I am not sure who disposes of the B5 for us but I do know that
it would be much easier if we changed to something else.
Jaclyn Pitts, HT(ASCP)
Histotechnician
Department of Laboratory Medicine and Pathology
Mayo Clinic
E-mail: pitts.jac...@mayo.edu
-Original Message-
From: his
You might try Background Sniper from Biocare or Background Buster from Innovex
- we process animal tissues exclusively and we use several rabbit primary
antibodies.
Tresa Goins, Ph.D.
Histopathology Supervisor
Department of Livestock
Bozeman, Montana
-Original Message-
From: histonet
Just wanted to qualify somewhat the observation that alcohol is a poor
fixative. Alcohol can be very effective as a primary fixative.
I agree formalin isn't likely to be displaced in this country soon, but aqueous
ethanol or methanol is a viable alternative that has merits worth considering.
This has been commented on several times by old fogey histotechs in the
past.
Any formalin variant takes some time to actually chemically alter (fix)
the tissue. Usually this is a minimum of 24 hours for a 3-4 mm thick
piece. In modern labs there is a focus on 24 hour or less turnaround
tim
Hi Histonetters!
I hope you had a fun and lucky St. Patricks Day.
March Madness has started and in addition to some great College
Basketball. I have been experiencing the madness as well. My phone has
literally been ringing off the hook with some great new opportunities.
All of these positions
Who do you get to discard it? Do you pay a fortune? j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, March 23, 2011 10:23
To: Pitts, Jaclyn S. (Jackie), HT(ASCP); histonet@
We do! Our pathologists aren't interested in using any other fixative.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pitts,
Jaclyn S. (Jackie), HT(ASCP)
Sent: Tuesday, March 22, 2011 6:46 PM
Dear Histonetters:
I recently read the following in an article on Medscape:
"Underfixation of breast tissue may lead to false negative ER results and
false-positive HER2 results. In these situations, the tissue is actually fixed
in 100% ethanol, which is used to dehydrate the specimens after f
Nirmala
We Use Vytec and after consulting with the city's public utility it is ok for
us to dispose of the waste with copious amounts of water down the drain. So, I
would contact you local water authority and see if you can do the same.
Regards,
Matt Lunetta BS HT(ASCP)cm
Longmont United Hospita
No.
And you're not welcome.
It has become almost a cliche to remark that nobody boasts of ignorance of
literature, but it is socially acceptable to boast ignorance of science and
proudly claim incompetence in mathematics.
-Richard Dawkins
On Tue, Mar 22, 2011 at 9:34 PM, wrote:
>
>
> Please
We have one in our gross room.
Caula Gill(HT)ASCP
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Tuesday, March 22, 2011 11:35 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet]
Is anyone using the Pax 2 antibody? Who is your vendor and could you share
some information about your procedure?
Thank you,
Andrea O'Brien
UroPartners
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We did until about 7 yrs ago when the hospital decided that all mercury
(thermometers and chemicals) would no longer be allowed which was fine with us.
We just went to 10% NBF since then.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.
We do not.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Pitts,
Jaclyn S. (Jackie), HT(ASCP)
Sent: Tuesday, March 22, 2011 9:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] B5
Hey all,
I
Dear All
I am looking for Rabbit Anti- NeuN antiboy to use it with anti-mouse OX42 for
double immuno staining.
Is there any one who have used any Rabbit Anti- NeuN?
Please let me know.
Thanks
Sohail
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Having read all the other comments, here's my 2.5 pence worth! Have you
tried a background reducing antibody diluent? You can get these from
DAKO or Menarini Diagnostics:
http://www.dako.com/uk/ar38/p107410/prod_products.htm.
Or MP-905-25 / MP-905-100, depending on the volume you require, from
Men
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