I've been attempting to do some multi-colour fluorescence labelling of FFPE
lymphoma tissue - usually I only use frozen tissue. It was all going smoothly
on some FFPE tonsil sections but with the lymphoma tissues I see punctate
nuclear staining with an epitope that should not be nuclear (CD32)
Good morning everyone,
I am curious to see what the standard protocol is on sentinel lymph node for
melanomas.
I need to establish a protocol at my institution and I have been reading a lot
of literature and it seems to be a wide variety of thinking out there from
bread loafing the lymph node
Hello Histonet!
Does anyone have an HE automated stainer protocol for bone marrows that they
wouldn't mind sharing? I'm looking at different protocols. It would be greatly
appreciated. Thanks.
Dawud
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Good Morning!
Found out a few days ago that next week we are participating in a summer camp
for high schoolers and it was decided to use skin this year for our examples. I
have lots of slides of skin sections from mice, rats, frogs, etc. and even some
human skin punches - normal and with skin
Hi,
We're upgrading our grossing areas with new workstations that require the techs
to interact with a keyboard and mouse. I'd like to get keyboard and mouse
covers to protect them from the usual hazards of grossing! But I can't seem to
find any big vendors that sell them...does anyone use
I just purchased a keyboard and mouse called Kleen Keys from American
Mastertech and they make a washable mouse as well. Our PA is so-so about
them...the keyboard is a little sensitive and the mouse is a little bigger than
the standard size mouse. Hope this helps- see link below.
Lisa
We use plastic wrap... Easy to change out and keep clean!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Do you completely wrap your mouse? Or do you leave the ball exposed?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Wednesday, June 01, 2011 1:16 PM
To: Dessoye, Michael J;
Actually, we put the mouse in a baggie. But the whole thing is covered.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: Rathborne, Toni
I had major problems with frozen trichrome years ago and Jamie Watson from GNF,
La Jolla said to fix in the Bouins and not fix in formalin and then mordant in
Bouins. (duh) I am not sure why I never tried that, but when I fixed in Bouins
for 10 minutes I got consistent trichrome on frozen skin
For the keyboard, what about using the silicon style? A lab near me uses them
exclusively. They are easy to keep clean, as they basically watertight - just
wipe down with a sani-wipe! And, they are fairly inexpensive - I got one from
Office Max (or was it Staples?) for $10.
Sent from my
Robin Dean asks: Can one re-use modified Davidson's solution for
fixing eyes and testes, or has it lost its potency/efficacy after
being used previously? Can you rejuvenate it by adding more acetic
acid? (how much?)
I would think that limited re-use would be acceptable - the
proportions of the
Amy,
I can't find the exact reference but I think either Balch or Taylor authored
the paper that was adapted for our protocol.
Level 1, 3, 5, 10 are H/E and extra section at level 5 is taken for Negative
serum control
Level 2 is S100
Level 4, 6 is PanMelanoma cocktail (Biocare Medical)
Level 7,
Histonetters:
What percentage of anatomical pathology cases in your facility are reviewed
by a second pathologist (intradepartmental consult)?
Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Center
(843) 777-2072
NOTICE: This e-mail
Around 10% (mostly requested by junior partners).
René J.
From: mwh...@mcleodhealth.org mwh...@mcleodhealth.org
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, June 1, 2011 3:59 PM
Subject: [Histonet] pathology intradepartmental consults
Histonetters:
What percentage of anatomical
Does everyone still receive printed requisitions with your surgical specimens?
How many of your OR's have a printer in each room? Our OR has a shared printer
for all of the procedure rooms, but would like to have a printer for each OR
suite. Our IT department is saying there would be problems
What are you using to check your fume hood's with for QC purposes?
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We have a service come out and inspect our hoods once a year.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont,
We installed small vanes in the area of maximum flow inside the hood and we
daily checked that the vanes were moving by the air. As to the actual flow in
f/min the hoods were checked annually by an outside company.
René J.
From: Amber McKenzie amber.mcken...@gastrodocs.net
To:
We still use paper.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
On behalf of a friend who is looking for a new tissue processor. She is asking
for information on a Leica ASB300. Any information would be appreciated.
Also, any pros cons of a long term lease/option to buy?
Thank you
Candy
Candy Bales, HT
Chief Histologist
The University of Texas Dental
I'm trying to determine the annual volume of glass slides produced
globally for a project I'm working on. I'm having no luck finding any
information. Any suggestions would be greatly appreciated!!
All the best,
Tj
Confidentiality Notice: This e-mail message,
Sorry, its an ASP300 processor.
Thanks
From: Bales, Candy A
Sent: Wednesday, June 01, 2011 4:05 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Leica processor
On behalf of a friend who is looking for a new tissue processor. She is asking
for information on a Leica ASB300. Any information
Internal consults are in the range of 2-7% in our institution. Some of these
are due to mandated second review of all new malignancies.
Retrospective review of cases by other pathologists, for example
conferences/tumor board/etc, is higher, in the range of 10-14% of cases.
Megan K. Dishop
Melanie S. White, MT(ASCP), Laboratory Supervisor, Systems/Anatomic
Pathology, McLeod Regional Medical Center asks: What percentage of
anatomical pathology cases in your facility are reviewed
by a second pathologist (intradepartmental consult)?
The one group I've worked with that addressed this
I will be out of the office starting 06/01/2011 and will not return until
06/07/2011.
Note: For Cytology issues, please call Molly (day) at 8-421-5487 or Eric
(eve) at 8-421-5405, For Histology issues, please call the general
histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran
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