Dear Histonetters!
I have human brain samples (age 70, HUntington disease) and my goal is
to quantitate changes in the number of neurons/glia cells with nuclei
staining.
I use NeuN IHC and Höechst. My first question is:
- Throughout the whole sample there are a lot of lipofuscin signal
Csaba,
I can't answer the fluorescent question but for light microscopic IHC I would
stain for the NeuN using peroxidase-DAB followed by a PAS for lipofuscin
followed by Mayer's or Harris's haematoxylin.
Result is Brown, pink, blue
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys,
hi every body
i have a confusing quistion reagrding Alcian blue pH 1 2.5 stains.
suppose you have 100 goblet cell react positive to pH 1 which mean all have
sulfated mucin. and 50 cell from other section react positive to pH 2.5 which
mean acidic type(sulfated or sialated). why these
We are having an issue with nuclear haziness on prostate biopsies in our small
urology laboratory. It occurs randomly, appearing on some levels and not
others
within the same block. Another scenario is that we sometimes see it occur only
in one small area of a core and the remaining tissue in
We still require a paper req with all specimens.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hello,
I have question regarding results I got using the Bielschowsky's
Silver Stain for plaques and tangles. The images and questions
pertaining to them are up at
http://www.ihcworld.com/smf/index.php/topic,6236.0.html .
I would greatly appreciate the input from some one who has used this
stain
We have one and it's been reliable. It's about 5-6 years old. It's also easy
to use.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax
We still use paper reqs.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildrens.org
Hello Histo folks
Hope everyone is having a great weekI am writing to get some info
on immuno kits for staining just by hand, like back when I first started
in this field. We are a small lab in Alaska and all of our immunos are
sent out of state, We are looking to do some of them here
Hello,
I am looking for a part time job to learn histology in the Raleigh/Durham area
of North Carolina. Maybe a few hours a week on Saturday or something like that.
I am HT registered.
Paula
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We also do our immunos manually - but we use no kits. I do not promote but do
suggest:
- Biocare Vulcan Fast Red as chromogen - air dry slide after counterstaining
with hematoxylin, then coverslip
- KPL for linkers
- concentrated antibodies; ready-to-use are good to check to see if the assay
How are you going to do that? Do you know how many laboratories there are? Do
you know the average slides/year for each?
Unless you know both parameters you cannot calculate the total national
volume.
I wish you luck!
René J.
From: Tonia Richmond tjrichm...@hughes.net
To:
Make sure that ALL your slides are properly dried in the oven and that NONE has
any remnant of water underneath before going into the oven to dry.
René J.
From: Jean Brinker j.brin...@att.net
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, June 2, 2011 6:03 AM
Subject: [Histonet] nuclear
(1) Mopec MB100 Grossing Station
http://media2.mopec.com/media/pdf/MBSeriesGrossingStationBrochure.pdf
Excellent condition.
Mark Sofferman,
President
American ReSource Medical
324 West Englewood Avenue
Teaneck, NJ 07666
P: 201.833.1550
F: 201.833.1575
There is an article on histology workload in the current issue of:
Arch Pathol Lab Med Vol 135, June 2011
Just placed on my desk today; I have only glanced at it. It may have some
information helpful to you.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
Dear Histonet members:
I am interesting in see the filopodia of the endothelial cells in the
corpus luteum of mouse. I have stained 25um cryostat sections of ovary
with an antibody agaisnt CD31 (PECAM) and alexa fluor and see it in the
confocal microscope, but I haven't succes. someone can
Would any recruiter who has a traveling PA available for 8-10 weeks coverage
starting August please contact me off-line?
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH
I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red
chromogen. Mouse primary antibodies usually give me better results than rabbit
or goat primaries.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We want to use laminin and Collagen IV to sort out the origin of some spindle
cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly
show laminin and collagen IV basement membranes vs. Fibrosarcoma which does
not. We need antibodies that will work in both cat and dog.
Margaret
We have used the Collagen IV listed below on canine but not feline, for
laminin we have not used the antibody from abcam on canine or feline but
the spec sheet states it works on most mammals.
Good Luck
Liz
Collagen IV
SouternBio
0340-01
UNLB
Goat
Laminin
abcam
We do ours manually also (about 20 different antibodies). We use a combo of
BioGenex and Dako. I like both set ups, but Dako is more expensive to perform
than BioGenex. I like that you can buy the in dividual components from
BioGenex instead of a whole ket from DAKO.
Dawn
-Original
I will be collecting bronchoalveolar lavage fluid (BALF) from mice and want to
do do a diff-quick stain on the fluid to characterize the leukocyte population.
My colleagues usually use a cytospin to concentrate cells onto a slide but I'll
be at a lab that doesn't have the equipment so I'm
For mouse and rabbit primary antibodies I like the Vector ImmPRESS kits. They
save time. For HRP substrates Nova Red looks too dull to me. I have had good
results with AEC and also with Vector ImmPACT DAB.
Jerry Ricks
Research Scientist
University of Washington
Department of Pathology
Jerry,
Firstly, I would not recommend fixing in ethanol since cells will shrink and
Romanowsky staining will be compromised (the cells stain too darkly since they
are smaller).
I would use a centrifuge to concentrate the cells and smear like a blood film
or if you do not have a centrifuge
We currently use paper reqs, but gasp coming soon, we will be going
paperless. I am not sure I am going to like this... ;o). Surgical staff will
enter the req in the computer, we receive the order the specimen and enter
into our AP system.
There are going to be growing pains!!!
Sent from
Working Ventana Probe Protocols!
Heres a good starting point when using the Kappa/Lambda/EBER Ventana Probes
on the Ultra:
Kappa Probe
-Depar 16 min
- Enzyme Protease 2 for 4 min,
-ISH (Kappa) Probe 4min
-Denature @ 85 degrees for 12min
-Hybe 1 hour
- 3 stringency washes for 8
What's up Tena!
Long time. The bone marrows will be fixed in B-5.
Dawud
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