Hi
Does anyone have a suggestion for decal solution/ time for (weakly)
ossified bone in fish larvae? Paraffin section tends to split due to
the harder tissue, nice longitudinal section are hard to get.
Tora Bardal
Thank you to everyone who responded to my decal solution for IHC
questionyour help was greatly appreciated...
Have a great day,
Vicki Gauch
AMCH
Albany, NY
-
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confidential information
Hello Tora,
We surface decal the trimmed block, for one hour, using a commercial product
called Rapid Decalcifier (Cellpath, UK). I don't know what it contains, other
than Hydrochloric acid, but it works a treat. Other alternatives for fish have
been Nair hair removal cream (applied to the
Hi,
I am using a mouse monoclonal antibody on PFA-fixed mouse brain slices, and the
Vector mouse-on-mouse kit. My background with the provided avidin-biotyn is
ridiculously high even after blocking endogenous biotyn. However, I get an
overall low background using an Alexa-conjugated goat
You can buy this type of thing too if you aren't the McGiver type. For
instance...from American Mastertech item # CPW04200E
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original
We us a scraper I bought at Home Depot called the floor buster.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. Any unauthorized review, use,
Danville Regional Medical Center located in Danville, VA has an opening for a
histotechnologist.
You may apply on line:
http://lifepointhealthcontent.com/custompage.asp?PageName=danvilletaleo
For Further information please contact our human resources department
434.799.4552 or 434.799.3719
Job
Hi Bret-
I've dealt with chondrocyte pellets a few times. It's a pretty half-assed
technique, but works pretty well if the pellet is relatively solid...if it's
not, maybe someone else can offer a useful suggestion :)
I was taught to use a piece of lens paper; pre-fold it in half several times,
Bret
We have done a bit of work with these types of samples, the first thing
we do is remove the formalin and add eosin to the container for about 5
minutes. Then we place the pellet (using a plastic disposable pipettor)
in a mesh bag and process on our tissue processor on a cycle that is set
at
I periodically use a scraper to get the wax off the floor under the microtomes
and I have housekeeping strip the floors a couple of times a year. We also use
a shop vac to vacuum up around the embedder and throughout the lab. We have
sticky mats at the entrance to the microtome stations and at
Just wondering how everyone is formally documenting validation of manual
special stains. If you have a template/form to share that would be
great. Thanks!
Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave,
Try EDTA for at least 24 hours
René J.
From: Tora Bardal tora.bar...@bio.ntnu.no
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, June 15, 2011 5:16 AM
Subject: [Histonet] decal fish larvae
Hi
Does anyone have a suggestion for decal solution/ time for (weakly) ossified
bone in fish
I will call myself old school and say, a slide works well and can be disposed
of in the broken glass box.
Now you can get your housekeeping dept to just shower scrub your floor and not
put wax on it. But the putty knife, especially on a broom or mop handle is
great, and easier on the knees and
Hello HistoNetters,
I was cleaning out a cabinet in the Laboratory, and came across 8 bags
of Allegiance S/P AccuTip Nonfiltered Pipett Tips (# P5049-15). They fit
a Oxford P-3000 or P-7000 pipetter. We no longer have the pipetter for
these tips. I am selling all eight bags for $20.00 plus
Hi,
We use sticky floor mats from market lab that help to collect any stray
paraffin. We use a scraper from market lab, it has a long handle so you
don't have to stoop over, to scrap up any paraffin that did not land on
the maps.
-Original Message-
From:
Dear Histonets,
How is it possible to validate a antibody on the Ventana Ultra or XT, when the
antibodys afre prediluted or made into a prep-kit?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Dorothy
I would assume that you would just run the validation as you would any
other antibody that is not a pre dilute. Run the antibody protocol on
the number of tissues that you normally do to validate, evaluate and
record the results.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Try about 30 (years)!
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda
Sent: Tue 6/14/2011 2:25 PM
To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Retirement
7 years compared to 8 months is
MTM is the only company that can sell the antibody.
http://www.mtmlabs.com/us/index.php
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
Dorothy,
Is your question related to the fact that a protocol will have the currently
active lot number and you want to use a different lot number? Or is this a
brand new antibody that has not been in use before and therefore has not ever
been validated?
Michelle
On Jun 15, 2011, at 11:41
MTM labs is the only place to get it IVD.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
I am downsizing my library and have the following books, mostly out of print,
for sale. If you are interested, make an offerf or any of them with enough to
cover postage also. I hate to just throw them away. Please do this to my
private email and not on histonet.
Carson: Histotechnology: A
Validating an Ab does not involve testing different concentrations, just a
known (+) tissue to be evaluated by the pathologist that should compare the
results with other tissues already reacted with the same Ab and protocol.
If the pathologist accepts the results, you have your validation.
René
Mrs. Carson,
Would it be possible to e-mail me the cost of these books? I would greatly
appreciate it and I am very interested.
Carson: Histotechnology: A Self-Instructional Text - 2nd edition.
2007
Bancroft and Cook: Manual of Histological Techniques and their Diagnostic
Application. 1994
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