[Histonet] restaining method of wright-giemsa stained peripheral blood slides

2011-07-11 Thread Shahram Sabeti
dear fellows,     I have some precious blood slides at hand but with a major problem,pallor, because all of them are unmounted and a long time has elapsed from their preparation .their restaining was frightening for me because a fault could result in  their deterioration.    do you have any expe

[Histonet] Re:Waste disposal

2011-07-11 Thread emily stebens
> We used Ventana Ultra in our lab. We sent a sample of the waste to our > safety department and they had the sample tested. They told us that it > could be dumped down the sink. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.u

RE: [Histonet] prostate bx containers

2011-07-11 Thread Horn, Hazel V
According to the CPT code book it is per specimen. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.arch

RE: [Histonet] prostate bx containers

2011-07-11 Thread Rathborne, Toni
Billing, I believe is per specimen, not per block. You may receive a specimen and divide it into 20 blocks, but it is still just one charge. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Se

RE: [Histonet] prostate bx containers

2011-07-11 Thread Johnson, Nacaela
Isn't it better to generate more blocks when so many cores are put in one vial? Therefore, the billing would be for the amount of blocks created correct? Thanks, Nacaela -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthweste

RE: [Histonet] prostate bx containers

2011-07-11 Thread Rene J Buesa
Your pathologist is right. You cannot. Billing is PER block René J. --- On Mon, 7/11/11, Jones, Laura wrote: From: Jones, Laura Subject: RE: [Histonet] prostate bx containers To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 1:15 PM We looked at these, but our Pathologist

[Histonet] specimen container analytic interference

2011-07-11 Thread shehnaz khan
Hi Netters, Please shed some light on the ffg: specimen container analytic interference. What should I do to comply with this standard? Thanx again S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mail

Re: [Histonet] pan B-cell marker for frozen mouse tissue

2011-07-11 Thread Adam .
B220 is a pretty good B-cell marker, although you're right that it's not restricted to the B-cell lineage. It's also expressed in some T-cells and NK cells, for example. Cells that are double positive for B220 and IgG or IgM are B-cells almost exclusively. CD19 is a good marker for mature B-cells,

[Histonet] pan B-cell marker for frozen mouse tissue

2011-07-11 Thread Michele Wich
I am trying to find the most appropriate pan B-cell marker for IHC on frozen mouse tissue. I know that CD45R/B220 used to be the most commonly used, but recent publications suggest PAX5 or CD19 as more restricted to the B-cell lineage. Does anyone have any other suggestions, or are these generally

[Histonet] Waste disposal

2011-07-11 Thread White, Lisa M.
For any waste disposal you have to check with city, state and federal regulations for all of the rules and then usually you will follow the one with the highest regulation to cover yourself. I work at a VA and have the good fortune to have a whole department to utilize for all of our waste disposa

Re: RE: [Histonet] prostate bx containers

2011-07-11 Thread Victoria Baker
The sites are individual according to the specimen requisition form and on the container. It is in how the labeling is done. Also when the pathologist reads them out they will be reading by site/position. I've never used these containers but have received specimens in a single container individ

Re: [Histonet] Test

2011-07-11 Thread histot...@imagesbyhopper.com
CONGRATS!!! :-D Sent from my iPhone On Jul 11, 2011, at 12:08 PM, wrote: > So I got married and my name changed (meaning so did my email) tried > sending out a broad email, but it didn't work. Just making sure I'm > updated now =) > > Happy Monday all!! > > > > Sarah Goebel-Dysart, BA, HT

RE: [Histonet] prostate bx containers

2011-07-11 Thread Jones, Laura
We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?" How is that handled? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf O

Fw: [Histonet] Re: Embedding Media

2011-07-11 Thread Tim Higgins
> Last comment on the paraffin conversion for me. > > You can use bees wax if you want in your processor and embedder that doesn't > mean you going to get the optimal results for every situation. Paraffin > types are a personal preference for most people or labs. It's a little more > hassle to k

[Histonet] Test

2011-07-11 Thread sdysart
So I got married and my name changed (meaning so did my email) tried sending out a broad email, but it didn't work. Just making sure I'm updated now =) Happy Monday all!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas

RE: [Histonet] Written SOP

2011-07-11 Thread Elizabeth Chlipala
I would think that you could potentially use an SOP for frozen sections in the clinical setting, such as following bloodborne pathogens standards. I would ban the use of freeze spray in the cryostat and that you may want to use a particulate respirator such as the #M N95 1860S, and other standa

Re: [Histonet] water

2011-07-11 Thread Rene J Buesa
Distilled water is nothing more than water without salts or ions. How do you get to that is irrelevant as long as the water, at the end, is "pure". Now days it is frequent to distinguish between "distilled" (meaning that the water first was evaporated using a heat source and later was condensated

[Histonet] For Sale - Mopec MB100 Countertop Grossing Station

2011-07-11 Thread American Resource Medical
For Sale - Mopec MB100 Countertop Grossing Station This is a used piece in excellent condition and half the price of new. http://media.mopec.com/media/pdf/Catalog2007smaller159.pdf This is a countertop unit. American ReSource Medical P: 201.833.1550

Re: [Histonet] Written SOP

2011-07-11 Thread Rene J Buesa
Really? Do you think that anybody is going to have an SOP for such an specific and narrow subject? Please remember that an SOP procedure is nothing other than the description of the method you use in a way that anybody else can follow it, and do the same thing always. I think that you will be be

RE: [Histonet] prostate bx containers

2011-07-11 Thread Dessoye, Michael J
We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org

Re: [Histonet] Re: Embedding Media

2011-07-11 Thread Rene J Buesa
In spite of many opinions on the contrary, I always found an error to use two different paraffins: = one for INFILTRATION and the other for EMBEDDING. You have to consider that, theoretical this is a mistake, because the paraffin that has infiltrated the tissue and REMAINS inside it sustaining it

[Histonet] Written SOP

2011-07-11 Thread Bascaramurty, Saro
Hi, I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal tissues implanted with human cells that are totally not screened for Hep B, HEP C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have one written, would you be kind enough to share with

[Histonet] water

2011-07-11 Thread Cynthia Pyse
Hi Histonetters I have 2 tech off on vacation so I thought I would take the easy way this time. Does anyone know the difference between distilled water system and the reverse osmosis water system? Could if effect the IHC staining by using one or the other? Usually I would research it myself but it

Re: [Histonet] Re: Embedding Media

2011-07-11 Thread histot...@imagesbyhopper.com
Amita, The paraffin that we use is also a Leica product. It was formerly sold under the Surgipath label, but since to companies have merged together, it now sells under the Leica name. It is a "beaded" pellet paraffin and has a version suitable for infiltration and a version suitable for embedd

[Histonet] Waste disposal

2011-07-11 Thread histot...@imagesbyhopper.com
For those who use the Ventana IHC and special stainers, how do you dispose of the liquid waste that is generated? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet