Re: [Histonet] restaining method of wright-giemsa stained peripheral blood slides

2011-07-12 Thread Rene J Buesa
Prepare a 1% alcoholic (100 EthOL) solution of acetic acid and immerse (one by one) the smears in it untilll all the color leaches out. Wash them thoroughly in distilled water, and restain them as usual with your Wright-Giemsa solution.René J. --- On Mon, 7/11/11, Shahram Sabeti

[Histonet] Remove me

2011-07-12 Thread Nele Degryse
Could you please remove me from the mailing list? Kind regards, Nele Degryse ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Remove me

2011-07-12 Thread Julia Koob
Yes, please remove me fromt he mailing list as well.. Julia Koob Technical Co-ordinator, Histology Laboratory North York General Hospital 416-756-6892 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

[Histonet] WASTE DISPOSAL

2011-07-12 Thread Sara Baldwin/mhhcc.org
Histonetters The stericycle chemists came to our place and looked at all our MSDS sheets that go with the Ventana by products, according to him it is not hazardous waste but most of the byproduct is liquid coverslip which is very oily, therefor he suggest we haul it off with the hazardous waste

[Histonet] Langerin antibody

2011-07-12 Thread Martha Ward
Hi all! I have been asked by one of our Pathology fellows to see if anyone is performing this antibody-Langerin- and if so, would you be willing to perform it for us.It is not something that we would be adding to our test menu at this time. Please let me know the fee and how many slides

Re: [Histonet] Remove me

2011-07-12 Thread ryrami...@cox.net
Remove me too. Sent from my Verizon Wireless Phone - Reply message - From: Julia Koob julia.k...@nygh.on.ca Date: Tue, Jul 12, 2011 7:16 am Subject: [Histonet] Remove me To: Nele Degryse nele.degr...@ugent.be, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Yes,

RE: [Histonet] Remove me

2011-07-12 Thread McMahon, Loralee A
you have to remove yourself. follow the instructions. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] What is a great manual mirotome

2011-07-12 Thread lscott
Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread Rathborne, Toni
We have the 2235 and some 2135's, and have been satisfied with all of them. Just the usual wear and tear problems seen over time. Do you have Leica service your current microtome? Is it always the same problem? Most service companies will offer a 30 day guarantee on their work. -Original

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread Elizabeth Chlipala
I find it hard to believe that you are not getting what you need from the Leica 2235, I don't have that particular model we have two RM2255 and one RM2145 and we just love them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread Wahlberg, Nikki
We use the Leica RM2255, RM2235, RM2165 and RM2265 and we love all of them! We are a research lab and are cutting paraffin and plastic. What are the specific issues that you are having with them? Nikki Nikki M Wahlberg Histology Lab Supervisor Preclinical Sciences Boston Scientific

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread sdysart
I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original

Re: [Histonet] What is a great manual microtomes

2011-07-12 Thread DKBoyd
We have two Leica's and love them a RM 2255 and a RM2235. No problems. A real work horse! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net

RE: [Histonet] What is a great manual microtomes

2011-07-12 Thread Breeden, Sara
What she said! RM2235 without question! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] What is a great manual mirotome

2011-07-12 Thread Patrick Laurie
I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread Bernice Frederick
I want my AO820 back! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re:

Re: [Histonet] What is a great manual mirotome

2011-07-12 Thread jsjurczak
We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125

Re: [Histonet] What is a great manual mirotome

2011-07-12 Thread Steve Mello
Leitz 1512. Great workhorse if u can find one Sent via BlackBerry by ATT -Original Message- From: jsjurc...@comcast.net Sender: histonet-boun...@lists.utsouthwestern.edu Date: Tue, 12 Jul 2011 19:47:27 To: Patrick Laurieforeig...@gmail.com Cc: Histonet@lists.utsouthwestern.edu Subject:

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread Bernice Frederick
I actually use a Microm HM315 and have never had issues with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Tuesday, July 12, 2011 2:47 PM To: Patrick Laurie Cc:

Re: [Histonet] What is a great manual mirotome

2011-07-12 Thread Paula Pierce
I still use AO820s!!! Keep them greased and oiled and they will just keep going, and going, and going   Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com

Re: [Histonet] What is a great manual mirotome

2011-07-12 Thread Victor Tobias
The old black ones, gotta love them. Most of the young techs out there probably have never seen one. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu

[Histonet] Histobath

2011-07-12 Thread Matt Mincer
Hey All, I am working with a company to develop a replacement for the Histobath. They should have a prototype to me by the end of the week. I am looking for a lab, preferably in the Chicago, that is willing to test and assess the product. Let me know if you are interested. Thanks Matt --

RE: [Histonet] microtome knife holder

2011-07-12 Thread Liette Tougas
Thank you Hugh and all others who replied. In fact what I really meant was rather a knife clamp, I guess, as on the older microtomes, where we insert either a regular knife. So my next question is: does anyone still have a knife clamp for either a 2030 Reichert Jung microtome or a Leica

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread JR R
I paid $30.00 for my pre-owned Spencer 820. Liked it so well I bought two. It has served as a virtually foolproof and indestructible workhorse for 22 years. I have produced, literally more than a tone of slides with it. Jerry L Ricks Research Scientist University of Washington Department

[Histonet] Histobath revised

2011-07-12 Thread Matt Mincer
Hey again. I was not refering to a tissue flotation bath. The Histobath was a product sold by Shandon. It had a chamber filled with isopentane or alcohol and was used for quick freezing tissue for a cryostat. Sorry if there was any confusion. Thanks Matt -- Matthew Mincer Tech One

RE: [Histonet] What is a great manual mirotome

2011-07-12 Thread Shea's
Our 23 year old Reichert Jung 2030s, with high profile blades. They are work horses Only one repair (the spring). Maintenance contract on a microtome? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.

2011-07-12 Thread Diane . Tokugawa
I will be out of the office starting 07/12/2011 and will not return until 07/14/2011. Note: For Cytology issues, please call Molly at 8-421-5487, For Histology issues, please call the general histology lab 8-421- 5408. ___ Histonet mailing list