Prepare a 1% alcoholic (100 EthOL) solution of acetic acid and immerse (one by
one) the smears in it untilll all the color leaches out.
Wash them thoroughly in distilled water, and restain them as usual with your
Wright-Giemsa solution.René J.
--- On Mon, 7/11/11, Shahram Sabeti
Could you please remove me from the mailing list?
Kind regards,
Nele Degryse
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Yes, please remove me fromt he mailing list as well..
Julia Koob
Technical Co-ordinator,
Histology Laboratory
North York General Hospital
416-756-6892
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Histonetters
The stericycle chemists came to our place and looked at all our MSDS sheets
that go with the Ventana by products, according to him it is not hazardous
waste but most of the byproduct is liquid coverslip which is very oily,
therefor he suggest we haul it off with the hazardous waste
Hi all! I have been asked by one of our Pathology fellows to see if anyone is
performing this antibody-Langerin- and if so, would you be willing to perform
it for us.It is not something that we would be adding to our test menu at
this time.
Please let me know the fee and how many slides
Remove me too.
Sent from my Verizon Wireless Phone
- Reply message -
From: Julia Koob julia.k...@nygh.on.ca
Date: Tue, Jul 12, 2011 7:16 am
Subject: [Histonet] Remove me
To: Nele Degryse nele.degr...@ugent.be, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Yes,
you have to remove yourself. follow the instructions.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
Hi,
Our small lab is looking for some advise on what microtome to replace a
fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200
and the Leica 2125. We are looking for reliability and have net gotten it
from the RM2235. Is anyone using either of these that would be willing to
We have the 2235 and some 2135's, and have been satisfied with all of them.
Just the usual wear and tear problems seen over time. Do you have Leica service
your current microtome? Is it always the same problem? Most service companies
will offer a 30 day guarantee on their work.
-Original
I find it hard to believe that you are not getting what you need from the Leica
2235, I don't have that particular model we have two RM2255 and one RM2145 and
we just love them.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
We use the Leica RM2255, RM2235, RM2165 and RM2265 and we love all of them! We
are a research lab and are cutting paraffin and plastic. What are the specific
issues that you are having with them?
Nikki
Nikki M Wahlberg
Histology Lab Supervisor
Preclinical Sciences
Boston Scientific
I am a manual tome junky! I like the Thermo Finesse (I think it's 325).
These are the old microms...they work like a charm =)
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original
We have two Leica's and love them a RM 2255 and a RM2235. No problems. A
real work horse!
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
What she said! RM2235 without question!
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I've always been a fan of the Leica microtomes, I've used all kinds, from
the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated
version is the RM 2235. I've also used the Microm (thermo-fisher-shandon)
HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I
I want my AO820 back!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie
Sent: Tuesday, July 12, 2011 2:40 PM
To: sdys...@mirnarx.com
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re:
We have tried the Leicas thru the years and found that post 2030's that our
arms were not long enough to turn the handwheel without completely
straightening our elbows. This led to soreness in our elbows. Their microtomes
kept getting larger and larger with the handwheel further back. The 2125
Leitz 1512. Great workhorse if u can find one
Sent via BlackBerry by ATT
-Original Message-
From: jsjurc...@comcast.net
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Tue, 12 Jul 2011 19:47:27
To: Patrick Laurieforeig...@gmail.com
Cc: Histonet@lists.utsouthwestern.edu
Subject:
I actually use a Microm HM315 and have never had issues with it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
jsjurc...@comcast.net
Sent: Tuesday, July 12, 2011 2:47 PM
To: Patrick Laurie
Cc:
I still use AO820s!!! Keep them greased and oiled and they will just keep
going,
and going, and going
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
The old black ones, gotta love them. Most of the young techs out there
probably have never seen one.
Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
Hey All,
I am working with a company to develop a replacement for the Histobath.
They should have a prototype to me by the end of the week. I am looking
for a lab, preferably in the Chicago, that is willing to test and assess
the product. Let me know if you are interested.
Thanks
Matt
--
Thank you Hugh and all others who replied.
In fact what I really meant was rather a knife clamp, I guess, as on the
older microtomes, where we insert either a regular knife.
So my next question is: does anyone still have a knife clamp for either a
2030 Reichert Jung microtome or a Leica
I paid $30.00 for my pre-owned Spencer 820. Liked it so well I bought two.
It has served as a virtually foolproof and indestructible workhorse for 22
years. I have produced, literally more than a tone of slides with it.
Jerry L Ricks
Research Scientist
University of Washington
Department
Hey again.
I was not refering to a tissue flotation bath. The Histobath was a
product sold by Shandon. It had a chamber filled with isopentane or
alcohol and was used for quick freezing tissue for a cryostat. Sorry if
there was any confusion.
Thanks
Matt
--
Matthew Mincer
Tech One
Our 23 year old Reichert Jung 2030s, with high profile blades. They are work
horses Only one repair (the spring). Maintenance contract on a microtome?
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I will be out of the office starting 07/12/2011 and will not return until
07/14/2011.
Note: For Cytology issues, please call Molly at 8-421-5487, For
Histology issues, please call the general histology lab 8-421- 5408.
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