If you use Clearium mounting media from Surgipath you can coverslip out of
isopropyl alcohol.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Tuesday, September 27, 2011 11:35 PM
Hello,
I was wondering if there are any = standards out there for the amount
of work a histology tech can do per year= ? We are currently doing
around 9000 cases per year = ;(approximately 45,000 blocks) with
manual special staining and automated (= old Dako
We have purchased from Master Tech in the past if you want to check.
Thanks Jane
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Tuesday, September 27, 2011 5:18 PM
To:
Henk - Mast cells, on tissue and/or cytospins, are pH dependent - 2.0-2.5.
Tissues are usually already fixed in formalin; cytospins can be fixed in
95% alcohol or formalin. I am sending our directions separately. The stain
should less than 5 minutes totally.
Cheryl
The standard benchmark for one HT that does everything as you describe is
around 9,000 blocks.
Under separate cover I am sending you data on the subject.
By the way, the standard for 9,000 cases would be around 27,000 blocks only.
You are preparing more blocks/case than the standard.
René J.
Why does methanol come in glass containers? Is anyone getting it from a
source that does not use glass? MSDS does not say it is reactive with
plastic. I'm sure someone out there can help me
William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
I've ordered it from Cardinal and Fisher and it comes in plastic
bottles.
Laurie
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Wednesday, September 28, 2011 7:49 AM
To:
Today there was a posting regarding Clearium (from Leica Micro-systems) where
it was said that it allows going directly from iso-propyl alcohol to
coverslippin, avoiding the use of xylene.
I did not know that so I found the MSDS for Clearium that reads:
Main hazards: highly flammable.
Feulgen and Rossenbeck developed this stain. Feulgen was the doctor,
Rosenbeck the labworker. Somehow the name Rosenbeck lapsed into oblivision.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag
The best configuration is connecting the oven directly to the fumes hood. I can
send you a photo of my installation if you want.
René J.
--- On Wed, 9/28/11, Duffett, Christopher duffe...@sjhmc.org wrote:
From: Duffett, Christopher duffe...@sjhmc.org
Subject: [Histonet] Microwave ventilation
We are considering purchasing a Peloris II. We are unsure of the validation
process of a processor, having not had to replace one in 15+ years. We
currently have 2 VIPs. For those of you using the Peloris, How did you
validate? how many runs, and what type of tissue did you use?
Thank you for
Mine is ducted directly into our ventilation system
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
On Sep 28, 2011, at 8:05 AM, Gudrun Lang wrote:
Feulgen and Rossenbeck developed this stain. Feulgen was the doctor,
Rosenbeck the labworker. Somehow the name Rosenbeck lapsed into oblivision.
This tells us something doesn't it?
___
Histonet
Hello,
I've been monitoring the discussion about Xylene.
No one has mentioned Latex.
I have the exact symptoms but from Latex.
Our lab is Latex free (???) but there are many items impossible to
remove.
Yes, we use powder free nitrile gloves.
Examples: floor tiles, paint on the
Select a series of different tissues (as diverse as possible) and run half
with your VIP and the other half with the Peloris. Do not identify which
processor was used for each block/section and give the sections to as many
pathologists in unidentified pairs as a blind test They will not know
Hello Histonetters
I have been reading about xylene, how dangerous it is.
Have you thought about using mounting medium made from Limonene.
Here is some info:
*AR-6504 Organo (Limonene) mount™:** *This mounting medium is made with
limonene a natural product from orange peels. It is good for
Hi all,
I wanted to thank all of you who responded to my question about my
xylene-sensitive employee and purchase of an air filter system. I got tons of
terrific information and enjoyed the discussion about xylene substitutes.
Those who responded gave me a lot to think about and raised some
HI, my lab purchased the Creative Waste Solutions Bench Top Formalin Recycler
a year ago in October and we're considering discontinuing use because of some
issues. I am posting this question hoping someone else has had similar issues
and maybe has resolved them or does something we're not.
This was a good discussion and a some little rough comment but we learn from
it. I have read all the feedback on xylene and its substitute and it gives us
a lesson to become more better histotechs. Just this morning one of our
administrator passed by near our processor while my co worker has
Might not be toxic but the fumes of citrus give me headaches and the smell
is terrible.. Im a slide bright girl, no odor, and non flammable
Hello Histonetters
I have been reading about xylene, how dangerous it is.
Have you thought about using mounting medium made from Limonene.
Here is some
Summary Explanation:
SLIDE BRITE is a revolutionary dewaxing and clearing reagent for histologic
techniques. It is a safe and effective alternative to xylene. It contains
no carcinogens,
no toxins, is odorless, and is classified as non-flammable and
non-hazardous. Its flash
point is above 140ºF
Do you use this product for processing and staining? Do you need to use a
particular type of mounting medium? How is it disposed of?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum
Sent:
Dear Netters,
I am posting this for a friend who is not on Histonet. The following is a link
to a job opening at Vanderbilt University Medical Center. Please respond to the
link if interested.
Amber,
I have a dermpath/ mohs lab and I use slide bright for everything. I have
used the product for 9yrs now, at least. Slide bright is in my processor,
H%E stain line and my deparrafin stain line. Definitly call and get
sample. I love it. I get mine from Mercedes medical, belair(advantik),
Has anyone ever used P16 with AP Red detection on the Ventana Benchmark?
Phyllis Jordan HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Toni,
Slide bright is everywhere xylene would have been. Proceesor, stainline, etc.
I use either TBS shur/mount( xylene based) or S mounting media (toulene
based). I think the xylene based media works better.
As per the directions it can be flushed down the drain with copious
amounts of water.
Histotechnology Congress 2012
will be Saturday January 14, 2012
Hotel El Pedregal, Aguadilla, Puerto Rico.
Spaces available for sponsors interested in participating please write to:
mvgeducationaresour...@gmail.com for more information.
--
*Lcda. Mary V. Guerrero, B.S., HtL, MBA*
Presidente
MVG
Hey Histonetters
Where can I buy Napsin A control slides we don't have any adenocarcinoma's for
internal ??
Thanks
Histology/Cytology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-996-0210, 0216, Fax 812-996-0232,
Pager 812-481-0897,
You are in a dilemma and that is why I never recycled formalin.
Recycling formalin by filtration seems better, but you will end with the
limitations you point out.
Recycling formalin by distillation involves neutralizing the product and in
both cases you will be exposed to formalin more than if
Hello All,
I'm starting my own lab and am trying to make the best use of my startup
package. I section rodent brain on a sliding microtome with a dry ice stage (AO
860) but would like to move to fresh frozen sections mostly for RNA work and
perhaps laser capture. I'm trying to decide whether
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