[Histonet] Histo Tech Wanted

2011-11-04 Thread Steven
Immediate opening for an experienced histology technician in a private dermatolgy laboratory in Springfield, MA. Full time, Monday - Friday, day shift. All aspects of routine histology. Frozen section experience a plus. Competitive salary and attractive benefit package, Contact: Shannon Page

[Histonet] Regarding MOHS....

2011-11-04 Thread Tom McNemar
Hello all, I have some questions for the community sized hospital that are doing MOHS. I would appreciated any input on the following questions: Do you have people specifically dedicated to performing MOHS? About how many specimens per patient do you receive? Are MOHS procedures performed

[Histonet] Statistical Information for GI Bx

2011-11-04 Thread SHANE NELSON
Does anyone know if and where I can find any statistical information on the average number of GI biopsies taken per patient? THANK YOU,   PATTI RUBEN-NELSON  H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net    

[Histonet] Re: Histonet Digest, Vol 96, Issue 6

2011-11-04 Thread Marilyn . A . Weiss
we currently use a form called Pactrak and then place it into the LMS system for tracking when things go out of our lab. these go usually to outlying areas. When specimens are brought to the lab we have paperwork that we call transmittal sheets. They have to be signed off by the person that

RE: [Histonet] Statistical Information for GI Bx

2011-11-04 Thread Nails, Felton
The patient condition should dictate what is taken, so the number will vary. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Friday, November 04, 2011 6:12 AM To: Histonet Subject:

[Histonet] low profile vs. hi profile blades

2011-11-04 Thread Nancy Schmitt
Hi- Please educate me on this. There seems to be a lot of personal opinion involved in regards to which type of blade to use. Thanks for your help Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if

[Histonet] Ventana Eber Probes

2011-11-04 Thread Evans, Andria B
I am struggling with my EBER probe on both the Ventana Benchmark XT's and Ultra. I was hoping that someone could help me out with what you are currently running with the new probe setup. Thank you! A. Harris (717)544-5457 ~ FAX (if it would be easier to fax the protocol) This email was

RE: [Histonet] low profile vs. hi profile blades

2011-11-04 Thread Monfils, Paul
In my opinion it doesn't make a lot of difference which type you use, since all you actually use is the 2mm or so that extends beyond the edge of the knife holder. What is crucial is that you use the type of blade your knife holder is designed for. If you try to use high profile blades in a low

RE: [Histonet] Ventana Eber Probes

2011-11-04 Thread Bauer, Karen L.
Hi Andria, This is our EBER protocol that we are using on our XT... With great success. Using the XT ISH Open Probes - Chromogenic procedure... Depar Enzyme: ISH Protease 3 for 12 minutes Probe HybReady Probe Auto Dispense: Then pick the probe that contains EBER Denature: 60 degrees C for 4

RE: [Histonet] Specimen Tracking for courier

2011-11-04 Thread Janice Mahoney
Yes, Call your Ventana rep. Vantage is able to track courier pick-ups and record what slides are sent, etc. Jan Mahoney Omaha From: cp...@x-celllab.com To: jennifer.b...@northwestpathology.com; histonet@lists.utsouthwestern.edu Date: Thu, 3 Nov 2011 11:13:02 -0400 Subject: RE: [Histonet]

RE: [Histonet] Staffing to work ratios

2011-11-04 Thread Janice Mahoney
I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE Date: Thu, 3 Nov 2011 13:03:44 -0400 From: stacy_mclaugh...@cooley-dickinson.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staffing to work ratios Hi, I know this

RE: [Histonet] Staffing to work ratios

2011-11-04 Thread Janice Mahoney
Sorry, i meant to say 5000 cases, not blocks. Jan From: mamaw...@hotmail.com To: stacy_mclaugh...@cooley-dickinson.org; histonet@lists.utsouthwestern.edu Date: Fri, 4 Nov 2011 06:05:22 -1000 Subject: RE: [Histonet] Staffing to work ratios CC: I have always used the 5000 blocks per

RE: [Histonet] Training and Competency Assessment for HE Slide Review

2011-11-04 Thread Janice Mahoney
A great way to do this is by using the results of the Histo QIP from CAP and the NSH. If you participate you get wonderful study and competency materials to use for routine HE's special stains and IHC. Jan Mhaoney Omaha From: joellewea...@hotmail.com To: diana.har...@viha.ca;

RE: [Histonet] Staffing to work ratios

2011-11-04 Thread Mike Pence
Sorry Jan, I posted before your repost. That makes more sense. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney;

RE: [Histonet] Staffing to work ratios

2011-11-04 Thread Mike Pence
That is only 20 blocks per working day (Mon-Fri)! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaugh...@cooley-dickinson.org; histo net

RE: [Histonet] Staffing to work ratios

2011-11-04 Thread Bernice Frederick
I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I can still do it after all these years. When I started I had 3 months

[Histonet] processing program for biopsies

2011-11-04 Thread Carol Bryant
Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station SolutionTime Temp P/VMix

Re: [Histonet] Staffing to work ratios

2011-11-04 Thread joelle weaver
Sounds more accurate to expectations in my experience as well. Sent from my Verizon Wireless BlackBerry -Original Message- From: Bernice Frederick b-freder...@northwestern.edu Date: Fri, 4 Nov 2011 16:36:28 To: mpe...@grhs.net; mamaw...@hotmail.com;

[Histonet] Aspyra LIS system

2011-11-04 Thread Joanne Clark
Hi Histonetters, have any of you out there had any experience with the Aspyra LIS system, specifically the anatomical pathology and cytology modules? Any feedback would be appreciated. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexcio Roswell, NM

RE: [Histonet] Staffing to work ratios

2011-11-04 Thread Janice Mahoney
I believe the NSH published standards a few years back that suggested the average for embedding is one minute per block and 2.5 minutes for sectioning. My personal opinion is that one minute is too fast for sectioning, unless you have already faced in and are just taking a section off the top

[Histonet] RE: processing program for biopsies

2011-11-04 Thread Elizabeth Chlipala
In my personal opinion I think the processing cycle is too short. I know that skin biopsies are tiny but we process all skin samples on longer processing cycles. Granted I'm in research but we use 45 minutes to 1 hour per station for all of our skin samples and they cut just fine. Liz

[Histonet] RE:Contents of Histonet Digest, Vol 96, Issue 8 processing program

2011-11-04 Thread Gilchrist, Eric
Was this protocol working in the past? Depending on the size of the tissue specimens, I would suspect that they are Not completely dehydrated and poorly infiltrated. Eric -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: processing program for biopsies

2011-11-04 Thread Blazek, Linda
What does look bad mean? In what way do they look bad? Do they cut well? What tissue processor do you use? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message-

[Histonet] Zeiss Microm Rotary Microtome HM 325

2011-11-04 Thread Lyn Stadler
Curious if anyone out there has one of these microtomes? We have one that needs repair of the Trimming Lever. The warranty has expired and I am trying to determine the most cost effective way to deal with it? Pay for the service call or purchase a service contract. Any

[Histonet] Circo antibody

2011-11-04 Thread Perry, Margaret
We are running out of our circo antibody from Iowa and did not find it listed in their bank. Are there any recommendations for a different source? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638

Re: [Histonet] Circo antibody

2011-11-04 Thread Jan Shivers
Margaret, You can purchase monoclonal PCV2 antibody through RTI (Rural Technologies, Inc.). Jan Shivers Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended

[Histonet] processing program for biopsiesIn-Reply-To=

2011-11-04 Thread Ruppert, Amysue
I agree that the time may be too short. I would suggest removing the 80% alcohol station and adding another 100%, with the time of 25 minutes. If you have a VIP tissue processor, you may also want to change the mix to fast, with such short times in the stations, the processor does not have any

[Histonet] Formic Acid Recipe

2011-11-04 Thread Jesus Hernandez
Dear all, I was having trouble staining Human embryonic palatal mesenchymal cells with multi-stain solution. My PI told me to use formic acid on the samples so that it could increase the permeability of the methyl methacrylate. I am not sure if this is enough information, but the cells were

RE: [Histonet] Formic Acid Recipe

2011-11-04 Thread Jack Ratliff
Hello Jesus! Try Sanderson's Rapid Bone Stain from Dorn and Hart Microedge (www.dornandhart.com). This is a metachromatic stain and should help you to penetrate the resin if heated at 60C and stained for 5-10 minutes depending upon section thickness. Rinse the slide in dH2O @ 60C and blot

Re: [Histonet] RE: processing program for biopsies

2011-11-04 Thread Kim Donadio
I agree with Liz, its too short for big skins. I always call skins chewy because thats what they will be if not processed enough.   In my experiance with this, skins that are small 1-2mm punch biopsies and shaves have done ok in a 15 min run for each with 30 min each for the formalins. Simular

[Histonet] paraffin temperature

2011-11-04 Thread Zoe rosa
Hello all, I need help with something basic: What is the melting temperature for paraffin? I appreciate your help, Thanks, Ismael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] paraffin temperature

2011-11-04 Thread Jennifer MacDonald
It depends on the paraffin. The melting point is usually on the bag. Sent from my iPhone On Nov 4, 2011, at 8:28 PM, Zoe rosa gaviotalopez...@hotmail.com wrote: Hello all, I need help with something basic: What is the melting temperature for paraffin? I appreciate your help,