Jesus,
The formic acid (5%) will decalcify the hydroxyapatite which can be very
hard and crunchy to cut but since you ground the section I guess you don't
care about that. I would be concerned though that the formic acid might
also cause you to lose staining of some cell components that depend
It depends on the microtome being used and how the blade holder is set up to
take high profile or low, many of them (at least the microms I have can take
either) on my microm to use low profile I leave the bar on the knife holder
so the holder is set to hold shorter low profile blades, I can take
In my experience even with the harder high profile blades for denser tissues
such as bone I still prefer to use permanent tungsten carbide blades I have
to send out to get sharpened. The high profile disposable blades seem to
vibrate too much for me even the heavier tungsten ones for bone.
Patsy
This was what I was going to suggest, I bet they let you participate in
HistoQip even if you are from Canada.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-Original Message-
From:
Corrie Vernick writes:
I am currently a histology student at Keiser University. I am doing a project
for my routine staining class about Celestine Blue. I've been able to find
information on why it was created, the chemical make up, and some of it's
uses including the trichrome stain. I am
Only 40 minutes in formalin is a problem.
Increase the formalin time to several hours and this should suffice for small
2-3mm punch biopsies of skin.
For larger skin sections the alcohol, xylene and wax times will need to be
tripled at least (as well as fixing the skins for longer (3-4 hours
With the hematoxylin shortage of a couple of years ago (real, not imagined
in about 2007-2008), several companies tried to come up with a synthetic dye
substitute.
A little background: Celestine blue (CI 51050, also known as Mordant blue
14) is a substitute touted many years ago (late
Hello, I am a histology technology student and I need
information on the melting point of the paraffin used in Microwave Processing…
Freida Carson stated in her book something regarding the paraffin temperature
set at 84 degrees. The class had a discussion regarding the same and concluded
that the
Hi there,
On an Instrumentation course at keiser, we had a discussion about the right
temperature fro the paraffin when the proceesing is done using microwaves. Can
some tell me what should be the ideal temperature with out damaging the
processing. The text book mentions 84 degrees, but
I believe that the higher temperature is needed to evaporate off the
isopropanol.
Just make sure that your tissue is well fixed.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the
Hello
I have used Celestin Blue-Haemalum (Mayers) considerably in the past as a
substitute for Weigert's and as you say the Celestin Blue stain does not keep
and is best made fresh for each use. Our method to simplify this was to make
separate double strength solutions of the Celestin blue
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