Cell Marque
0.1 ml concentrated 367M-14
0.5 ml concentrated 367M-15
1 ml concentrated 367M-16
1 ml prediluted 367M-17
7 ml prediluted 367M-18
We use it routinely and it works great.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of
Sorry I should have been more specific. I am not looking for a mouse monoclonal
anti-human IgG4 antibody. I need human IgG4 to run as an isotype control for
another primary mouse monoclonal that is an IgG4 isotype. The only company I
have found so far that sell purified Human IgG4 is in England
Hi,
If you are concerned about the thickness of the sections being accurate
to the setting, I would suggest picking up a cheap micrometer from a
hardware store. (OK perhaps not cheap as you will want a quality one, but
the cost of these isn't terrible.) You can't really measure 5 microns (or
First try Sigma Aldrich
William DeSalvo, B.S., HTL(ASCP)
From: sharron.l...@immunogen.com
To: loralee_mcma...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu
Date: Fri, 18 Nov 2011 08:22:05 -0500
CC:
Subject: [Histonet] RE: Source of IgG4 for Isotype control
Sorry I should have
Hi all ,
Wondering if anyone has experience with these antibodies on hamster tissues:
- Cathepsin B or a pan-cathepsin Ab if on exists
- MMP-3, MMP9, MMP12
- neutrophil elastase
Thanks for any info.
Brett
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck Co., Inc.
PO Box 4, WP-44K
West
Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and
Digestive Health Specialists is looking for a certified HT or HTL to
run their laboratory. Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred.
Great opportunity for a Histotechnician in a brand new laboratory!
Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice
located in Lafayette, Louisiana, is looking for a certified HT or HTL to
run their newly constructed laboratory. Candidate must be ASCP certified
and CLIA
Good Morning,
Does anyone know of a *mouse* xGFAP that will work on rabbit tissue?
Thanks in advance,
--
Jan Shivers
Senior Scientist
IHC/Histology/EM Section Head
Pathology Teaching Program
UMN Veterinary Diagnostic Laboratory
University of Minnesota College of Veterinary Medicine
1333 Gortner
Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and
Digestive Health Specialists is looking for a certified HT or HTL to
run their laboratory. Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred.
Hello everyone!
I have a problem in processing rat paws to paraffin embedding.
I need to process and cut the whole paw (except fingers). So, first I need
to decalcify the paw and then process it to paraffin embedding.
My problem is that when I try to cut the paw I can not get a good section
Catia
Its going to take about a week to decal those types of samples in formic acid
and in addition to that the processing cycle needs to be considerably longer.
By rat paw, I'm assuming you are mean the front paw or is it the rear ankle
that you need to process. First of all the samples
Hi, anyone has experience in tissue clearing? i want to clear spinal cord
after fixation. Thanks. YJ
--
Truth Shall Make You Free
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hi Histonetters!!
I hope you are gearing up for a fun Pre-Thanksgiving weekend. I have a
great job opportunity to tell you about. I am currently working with
one of my best clients and they are in need of a histotech. I refer to
them as one of my best clients because they have very low turnover
Do you have access to a confocal microscope or one that does Z-stack imaging?
You can find out the thickness of your tissue sections that way (after you've
mounted them on a slide of course).
Regards,
Merced
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
YJ,
You would probably be interested in this:
Scale: a chemical approach for fluorescence imaging and reconstruction
of transparent mouse brain. Hiroshi Hama et al., Nature Neuroscience
14, 1481–1488 (2011)
---
Message: 1
Date: Fri, 18 Nov 2011 09:17:36 -0800
From: aget liang
I have had folding and bubbles occur periodically with PFA fixed, sucrose
cryoprotected frozen brain tissue I receive from other labs. I streak a
distilled water moistened brush across the slide then pick up the frozen
section on the moistened area. It comes out flat and smooth every time. I
How are those of you in Technical Component labs handling your PT
testing ? And if you are grading these slides how are you setting the
criteria and what are you looking at ? Thanks
Meredith Hale HT (ASCP)cm
Operations Liaision Director and Education Coordinator
Caris Life Sciences
Hey Connie,
Remember that we experienced a drastic heat wave all over the country and in
some parts there were rolling blackouts.
The product may have been stored in a warehouse that experienced a rolling
blackout and the increased temperature messed up the paraffin which then
re-hardened and
Well, I have used all of the usual suspects over many years but, this one has
taken me by complete surprise!
If anyone can explain the rationale of the reaction, I would be most interested.
One for J Kiernan, I think?
Ah, yes: Sca/e: a chemical approach for fluorescence imaging and
-Marian
Begin forwarded message:
From: Marian Powers mpow...@dpspa.com
Date: November 16, 2011 12:51:56 PM EST
To: histonet@lists.utsouthwestern.edu
Subject: IHC control
Hi: Would anyone have a hereditary nonpolyposis colorectal cancer control,
(for MHL1, MSH2, MSH6) to share?
Did anyone know what is the buffer system for the 4M urea? it is important
to adjust and maintain pH to 7.7 according to the paper:
Scale: a chemical approach for fluorescence imaging and reconstruction of
transparent mouse brain
2011/11/18 aget liang agetli...@gmail.com
Hi, anyone has
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