We usually go by first asked days off. (We keep a calendar for this purpose)
But for holidays we rotate. If someone had the day after Thanksgiving off last
year they let the next in line have the choice.( I keep the previous years
calendars for checking) If no one else wants it they can then
I've witnessed that granting time by seniority lets you open to abuse. I know
of an employee with high seniority who takes the whole week after Christmas
every year. Our policy allows only one person off per day per shift. So then no
one else ever can take off at Christmastime. Her peers
Good morning Histoland!
I would like to tell you about a wonderful job in a great lab with very special
people. I am retiring but my job isn't! I run the histo core for the Hermelin
Brain Tumor Center at Henry Ford Hospital in Detroit, MI. I have a group of
Neurosurgery Research
How do you handle PRN's? If they turn in time off do you grant them before the
FT staff or don't worry about their days since they are fill in people? Do
PRN's count as having 1 person off at a time along with the FT staff if you
only allow 1 person off per day?
-Original Message-
We have a list, that can be filled with holiday-wishes. We arrange a special
date (for summer holidays e.g. in february) until then the wishes had to be
declared. If there are no troubles concerning the number of workers
simultanously off, the holidays are fixed. If there are troubles, we speak
Hello, Histonetters,
To continue my (sad) string posts about CryoJane problems, we have now
switched to the 4X slides, and we still observe the tape pulling pieces of
the section off the slide. Sometimes the pieces are very small, sometimes
they are large, and at times a whole region of the
Hello all,
I am studying for my HTL and have a question that has been a problem for me
and was wondering if anyione out there can help. What cuases paraffin blocks
to crack ? So far i have come up with it has to do the crystal formation
during cooling, and I have seen some Histonet articles
Hi,
I was wondering if anyone has used the Biocare TB antibody on the Ventana XT
would be willing to share their protocol. We are having trouble getting this
antibody to work for us without background staining. Thanks,
Mandy Bell HTL(ASCP)
Community Hospital of the Monterey Peninsula
Rapid cooling is the problem, but I do not think that -5ºC can cause it. The
effect is more noticeable in large but thin blocks.
René J.
--- On Wed, 1/4/12, Brian foster brian1...@email.com wrote:
From: Brian foster brian1...@email.com
Subject: [Histonet] Paraffin Blocks Cracking
To:
I agree however; leaving blocks on the cold plate too long will also cause
cracking of blocks even at -2C.
- Original Message -
From: Rene J Buesa rjbu...@yahoo.com
To: histonet@lists.utsouthwestern.edu, Brian foster brian1...@email.com
Sent: Wednesday, January 4, 2012 11:50:13 AM
Is anyone currently using the EPIC-beaker LIS system? Can you contact me if you
are?
Thanks
Jessica Vacca
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Hi Douglas,
We have a cryojane system, but do not use it too often. So this might not be
expert advise, but I do remember that we would sometimes flash the slide more
than once. I was just checking in the procedure manual that came with the
equipment and it does not mention to do this.
When I did it, seniority first, rotating holidays, but birthdays around
holidays were a sore spot. I usually gave the birthday and one day, but not a
whole week if it fell within a holiday week.
This allowed those who wanted to rotate holidays to do so, but they could only
take that one. No
I have also noticed that the temperature in the lab makes a big difference. Now
that it is colder outside and some heaters kick on check your temps.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brian
Also, using 100% alcohol on the cold plate induces cracking. Freeze spray does
too, although some people will never admit to using it. Both of these things
induce the rapid cooling effect.
Toysha N. Mayer, MBA, HT (ASCP)
Instructor
Program in Histotechnology
School of Health Professions
MD
I would also be interested in any replies concerning EPIC-beaker.
Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104
-Original Message-
From:
I've read somewhere (?), that too slow hardening is the culprit for
sprinkled blocks. I can also imagine, that the type of paraffine and special
additives can make a difference.
Our coolplate is set at -10°C and had never problems with cracking paraffin.
I think it's similar to freezing. The
I start with the holidays. We rotate the major ones every year. When I set
up the private lab I used to work at, I had it put in the H.R. policy that
an employee could take 2 of 3 end of year holidays (Thanksgiving, Christmas,
New Year's) on a rotating basis. If no one else put in for those
We are currently starting up some IHC on frozen tissue sections. After
staining with different fluorescent antibodies, we end with applying DAPI
w/Prolong gold and then coverslipping. We would like to seal the coverslip so
that we can keep the slides longer.
Any suggestions on where and how
Histonetters:
I waited a few days to see how others might weigh in after this information
was posted. Call me crazy, but I expected quite a bit more reaction from
our community. How is it that such a signifcant change in how IHC testing
may be conducted and will be paid for in the future can
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