I would be worried too.
Wearing gloves in the lab is always a good practice and
I believe, a requirement in my lab whenever handling reagents or
the possibility of coming into contact with reagents.
You have the right to wear gloves if you want.
Dana Settembre
University Hospital - UMDNJ
How does everyone store tissues that are at -20 or -80? Currently we wrap the
tissue well in foil and place in a labeled cassette. If shipped out, we double
bag as required. Recently, a concern has been raised about the cassettes being
a safety issue due to the fact that the tissue could be
There is no evidence in the literature about skin cancer produced by xylene,
although dermatitis are well documented.
Regardless you should use gloves whenever your hands can get in contact with
any chemical as a good safety practice. If your colleagues do not want to use
gloves, that is their
If you can get a hold of them, try using Nitrile gloves as these have a higher
chemical resistance than latex. I use them and change every 30 minutes to
avoid breakthrough.
Debbie Faichney
Institute of Aquaculture
University of Stirling
UK
-Original Message-
From:
Tissues in my tumor bank (-80ºC) were kept in small (1inchx2inches) labeled
zip-lock bags. I do not understand about your concern regarding tissues being
exposed to staff.
Inside the bags I used, the tissues were not exposed. If they were taken out
for any study or procedures, universal
I work in a research lab, but I use histogel all the time to make FFPE blocks
of cell culture material. Here is my procedure:
1. you need about 5X10^ cells per pellet
2. spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube
3. aspirate and resuspend in 15ml NBF and fix
I forgot to mention, we prepare these specifically for IHC; so yes, you can do
IHC on them.
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
From: Kim Merriam kmerriam2...@yahoo.com
To: Turner, Leandra lturn...@seton.org; histonet@lists.utsouthwestern.edu
You might want to go to something with even greater chemical resistance - thin
nitrile is not recommended for use with xylene:
http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx
According to our xylene MSDS: The substance may be toxic to
Allied Search Partners has been retained for the following searches in
Florida. Please forward this along to anyone who you know that would be
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Do y'all run the temp verifier slides for the quarterly maintenance for the
Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months,
esp if you have multiple pieces of equipment.
___
Histonet mailing list
Nitrile gloves are recommended for all the chemicals we use in our lab except
acetone - for acetone latex is recommended.
We also coverslip by hand but we wear nitrile gloves without exception.
Tresa
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
For those of you who use reagent alcohol, have you ever experienced any
problems in processing or staining, such as artifacts, crystals forming, etc??
How long are unstained slides usable? Do any of you pick up extra sections
from a ribbon if the tissue is minimal ? We do and have used them
I don't know of any evidence that xylene causes skin cancer. Concern
is with absorption through the skin. The most likely problem is with
the bone marrow - leukemia and related diseases - from aromatic
hydrocarbons (xylene, toluene, benzene) - which of course are present
in resinous mounting media
Hi Jenny:
Xylene can pass through the skin via the lipids that surround the skin
cells, just the way the medicine in nicotine patches, sea sickness
patches and birth control patches can.
How much can enter your system is not known to me but you should wear
resistant gloves, maybe even double
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We use nitrile to coverslip at our lab. Several of us including myself
have latex allergy as well as the latex breaks down quickly. Most labs
require you to use gloves handling any chemical substance. I have one
tech who has years of experience that will suffer greatly at 1 drop of
xylene or
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Hi,
We freeze store muscle bx's brain tissue at -70°C since the neuro lab was
started (around 1980). We started using cryogenic containers after we found
that over time the tissue would dry out with any other type of storage we
have tried them all.
Hope this helps,
Sharon Allen
Senior
Debbie:
Are the 30 min official? Any standard procedure that states 30 min as
maximum?
If you can get a hold of them, try using Nitrile gloves as these have a higher
chemical resistance than latex. I use them and change every 30 minutes to
avoid breakthrough.
Debbie Faichney
Institute of
Hi Dorothy,
I have used reagent alcohol for years both with staining and tissue processing.
I have never noticed any artifacts or crystals due to use of reagent alcohol.
One thing that you should know is that all denatured alcohols are not the same
so make sure that you buy a denatured
Dorothy, saving cut slides forever is not a good idea in my opinion. They
attract dust, fungus,Bacteria, all kinds of artifacts on top of loosing antigen
specificity. Cut slides is not the answer to me. I had to deal with this a
couple if times hopefully your pathologist will understand it's
Hi Dorothy
If you want to prolong the viability of pre-cut unstained slides,
firstly dry them, then dunk the slide into a bath of molten paraffin wax
deep enough to submerge all of the tissue section, then remove it and
allow the wax coating to solidify. This will keep the environment out of
your
Hi all,
My 2 cents worth, just be careful which gloves you wear. The reason for latex
residues appearing on the back of the slides is that you are wearing the wrong
gloves.
Nitrile gloves are what you need to be using, latex will dissolve in the
xylene.
Remember (1):
Disposable
Wow,
That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene,
whereas Ansell's Nitrile gloves are (see
http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory
Message: 15
Date: Wed, 22 Feb 2012 15:26:43 +
From: Amber McKenzie amber.mcken...@gastrodocs.net
Subject: [Histonet] Temp verifier slides - Ventana equipment
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
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