I think it's more to have consistency, rather than, say, a physical reason.
My opinion.
Example:
- Tech A put the shiny side down on the flotation bath, and picked up the
sections on the slide, and did an HE.
- Later in the day, the pathologist needs additional levels or some special
stains
Okay, I can't stand this! Although I always make sure my ribbon is placed
shiny-side-down on the water bath, sometimes the only section this poor ol'
tech is able to get might land upside down. In that case, I just tell the
pathologist the turn the slide over on the 'scope. Kinda like
We use soy agar slants that we get from our Micro department. We just melt one
as needed.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original Message-
From:
We use Cardiac Troponin I or Cardiac Troponin T all the time (pigs). These are
also in mice.
Regards,
Merced
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks
[amosbro...@gmail.com]
Good morning!I am Carmen from the University of Valencia. Now I am performing
immunofluorescence of mouse decidua. I was having problems with the
autofluorescence so I found a sudan black protocol and I probed it, I
incubated the tissue 30 min with sudan black after the staining, but I
The heating I was referring to was the 60C the paraffin must be melted at
to embed the tissue. We also use a vaccuum oven to paraffin embed so maybe
that also damages the antigen. We usually leave tissue up to six hours in
the paraffin oven before embedding it at room temperature.
Emily
The
Placing the shiny-side of the section on the water surface you assure that the
sections corresponds to the block, of course you cannot turn-around the
section. Also it will allow water tension to expand the section better and
assures a better adhesion to the slide surface.
If the section is in
My mentor, Nick Roman, told me that sections adhere to the slide better if they
go on shiny side down. Brenda Disbrey's HISTOLOGICAL LABORATORY METHODS says
that laying the sections on the water bath or water droplet shiny side down
makes it easier to remove creases. Benno Romeiss'
Hi everyone,
Does anyone have a list of the shelf life of solutions, chemicals etc.
used in Histo staining methods that is acceptable for CAP accreditation
would share the information?
We are trying to get a list together but different sources often
conflict. I would appreciate any information.
Such a list does not exist because the shelf life of every reagent (either dry
or in solution) is determined by the manufacturer depending on their own
specifications or desired turn-over buying/replenishing rate.
René J.
--- On Wed, 2/29/12, Sharon Allen sal...@dsmanitoba.ca wrote:
From:
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Wed, 29 Feb 2012 18:12:50
The castle of Lord Clifford was built at the opening of the gorge, and it
commanded an enchanting view of the valley below (c) cole
Allied Search Partners is currently looking for a qualified applicant for a
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It just occurred to me (after prompting from a friend on Histonet who
knows my Secret Identity) that you might not know who nmhisto@comcast
is. That would be me. I'm transitioning to my home base and get
Histonet there. That explains the strange posting from that person,
does it not? But, yes,
Sally, it was easy - aren't you the only histotech in New Mexico?
Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden,
I am out of the office until 03/01/2012.
I am out at a customer site all day and without access to email or
voicemail. I will return your message as soon as possible. If this is an
emergency, please text my cell at 847-257-3111. Diane
Note: This is an automated response to your message
Anyone interested in a used, working Cryostat, contact me directly:
Leica/Reichert-Jung CRYOCUT 1800
Rolly Perez, HT(ASCP)
Histology Supervisor
MedSurg Pathology Associates, Inc.
Tigard, OR
503.443.2157
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It should not interfere. I use Sudan Black a lot. What concentration do you use?
Regards,
Merced
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia
Pascual
Who wants to look at Cell bottoms all day!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked
Consistency, Consistency, Consistency.
1. It is easier to place sections on the water bath with the shiny surface
down (MOST times).
2. Staining-wise, for all stains that I have tried, it does not matter
(HE, PAS, Perl's Trichrome to name a few).
3. When you are matching blocks
Does anyone know if there is a Sunquest CoPath listserv?
We are upgrading to v.6, and I would like to mine it for information
about this new version.
thx,
David Watkins, MD
Department of Pathology
Baylor University Medical Center
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