Hello Everyone
I have worked in histology for 41 years and the only reason we ever put
sections shiny side up is when we want to look at back to back sections:
We would take the first section and put it shiny side up in the bath and
the next section immediately after would be placed shiny side
Is it ONE particular case that is giving you problems, or ALL cases of
amyloid? Maybe just the control?
If the amyloid is a large deposit, that has been in the patient for a long
time, the beta pleats can get warped, and the Congo red will be very pale to
no staining. In those cases, we:
-
Peroxidase, the enzyme you want to block with hydrogen peroxide, is a protein
and, as such, should be cross-linked by formalin if the tissue is properly
sixed.
In consequence, you should eliminate the crosslinkage to fully expose the
enzyme before blocking it.
Therefore, you should do HIER
I have an old Shandon tissue processor that is so old, they just called in
tissue processor, I think Kennedy was in office, and the serial number is -10.
THe machine has been donated and passed down and I am having a hard time
getting to work. I t worked once, but now it just keeps giving me
We have run into an interesting scenario and wondering what the experts
think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one
particular microtome. Within the past month, the hematopathologist has felt
the sections are thicker than the usual 3 microns. I had our
Has anyone out there done AKT and pAKT antibodies for IHC. I have been working
on them for about a month now and they are still not working right. I hope
there is someone out there that can help me???
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating
Make sure all levers and parts of the knife holder are tightened securely, but
do not overtighten -especially on the blade holding plate
F. Long
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb,
Some peroxidase-like enzymes in macrophages (probably catalase) and DAB-binding
activities in erythrocytes (hemoglobin) are not blocked by formalin. The best
way to block this activity was incubation with methanol-peroxide (99 parts of
methanol-1 part of 30% peroxide) during de-waxing. 3%
I've used cell signaling 4060S XP for pAKT, and it works really well.
From: courtney.pie...@quintiles.com
To: histonet@lists.utsouthwestern.edu
Date: Fri, 2 Mar 2012 13:31:11 -0500
Subject: [Histonet] AKT and pAKT
Has anyone out there done AKT and pAKT antibodies for IHC. I have been
Cold temperature will shrink the block and determine thinner sections, not
thicker sections. The problems has to be on the mechanics or perhaps the block
is hotter than you think it is.
René J.
--- On Fri, 3/2/12, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote:
From: Webb, Dorothy L
dear all on histonet
I'm going to setup a new histology lab. in Egypt. I need to contact with you to
advice me about the best and comfotable facilities
microtomes- processors-automatic stainers and so on .
Mohamed
Ass. Lec. of histology
Faculty of Vet. Med.
Cairo University
Egypt
It would be better if you contact a local sales manager knowledgeable of the
local characteristics and ask him/her this question. From here you will
probably receive advises about instruments that probably are not available in
Egypt.
René J.
--- On Fri, 3/2/12, mohamed abd el razik
Love your question. Hate to hear that you are having a issue. My two cents
follow:
Yes. The amount of time for a faced block will effect the section thickness.
The cells become bloated if they Sit to long. feel free to have a Friday
laugh on this one.
Anyway. If you havnt changed the stain
A stain I have never had a problem with. However I did have the good
fortune to listen to Culling many years ago on the use of 1% periodic acid
for PAS staining and he insisted periodic acid, no matter what the
concentration, should be made fresh every time you do these stains e.g. PAS
and PAMS.
There is a way to post pictures on histonet but just not in the email
messaging. The instructions for doing this are found on the Histonet
website.
Speed does affect the thickness of sections. It pays to turn the flywheel
at a steady, slow pace and not NASCAR racing speeds I have sometime
Hello Histonet Users,
I have decided that I should ask the experts. I regularly thick section muscle
sections in Embed 812 for bouton identification. I normally use Toluidine
blue. I am having trouble differentiating between boutons and a type of
vacuole that occurs within the same tissue
I will be out of the office starting 03/02/2012 and will not return until
03/05/2012.
In my absence please ask for Mary . If this is urgent or you need to
speak to me directly you can contact me on my cell phone number
858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or
From the CAP site:
The Benhold Congo red technique does not yield reproducible results and should
be avoided
and
the alkaline Congo red method of Puchtler, et al.,7 remains the gold standard
for the demonstration of amyloid in tissue sections.
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